In vivo anti-hyperuricemic activity of sesquiterpene lactones from Lychnophora species

Bernardes, Ana Catharina Fernandes Pereira Ferreira; Coelho, George Luiz Lins Machado; Araújo, Marcela Carolina de Paula Michel; Saúde-Guimarães, Dênia Antunes

doi:10.1016/j.bjp.2018.12.008

Cited by 15 publications

(7 citation statements)

References 24 publications

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“…Five concentrations of hydroalcoholic extracts of species from Ecuador and Peru were tested, and Allopurinol as a reference drug, which prevents the formation of uric acid and is the mainstay of prophylactic treatment for hyperuricemia in patients undergoing chemotherapy 10,11 .…”

Section: Discussionmentioning

confidence: 99%

In vitro inhibition of xanthine oxidase by hydroalcoholic extracts of Corynaea crassa Hook. F

Barrera

Gaitén

2023

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Corynaea crassa is a hemiparasitic plant native to America, used for the treatment of erectile dysfunction and has been shown to have antimicrobial properties. However, it lacks other biological studies to justify its use in traditional medicine. The objective was to examine the in vitro inhibitory activity of xanthine oxidase in hydroalcoholic extracts of the species from Ecuador and Peru. The extracts obtained by maceration and the Allopurinol used as the reference drug, at concentrations of 10, 30, 40, 50 and 60 µg/mL, were tested to measure the degree of in vitro inhibition of xanthine oxidase employing spectrophotometric determination at 295nm, which is associated with the formation of uric acid. The two extracts showed significant inhibitory activity on xanthine oxidase in a concentration-dependent manner, with the highest percentages being observed at the highest concentrations, being higher for the extract from the Ecuadorian species with enzyme inhibition percentages comparable to Allopurinol. Median inhibitory concentration (IC50) values of 15.35µg/mL and 17.42µg/mL were observed for the extracts from Ecuador and Peru, respectively, although the activity was more notable for the reference drug, which was shown to be an IC50 of 12.21 µg/mL. The results concluded the basis for the potential use of C. crassa in the treatment of hyperuricemia. Keywords: xanthine oxidase, hydroalcoholic extracts, Allopurinol

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Section: Discussionmentioning

confidence: 99%

In vitro inhibition of xanthine oxidase by hydroalcoholic extracts of Corynaea crassa Hook. F

Barrera

Gaitén

2023

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“…To evaluate the anti-hyperuricaemic activity, the model described by Murugaiyah and Chan [ 42 ], Ferrari et al [ 43 ] and Bernardes et al [ 44 ] was used. Animals were initially deprived of water and food for 12 h. Hyperuricaemia was achieved by treating animals with a single dose of potassium oxonate (200 mg/kg, i.p.)…”

Section: Methodsmentioning

confidence: 99%

Chemical Analysis of Eruca sativa Ethanolic Extract and Its Effects on Hyperuricaemia

et al. 2022

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In vivo assays and chemical analyses were performed on the ethanolic extract from leaves of Eruca sativa. UHPLC-ESI-QTOF analysis confirmed the presence of glucosinolates and flavonol glucosides. The major flavonoid of the ethanolic extract, kaempferol-3,4′-di-O-β-glucoside, was isolated, a HPLC-DAD method developed and validated to quantify its content in the extract. In vivo experiments were carried out on Wistar rats with hyperuricaemia induced by potassium oxonate and uric acid. A hypouricaemic effect was observed in hyperuricaemic Wistar rats treated with ethanolic extract at dose of 125 mg/kg and kaempferol-3,4′-di-O-β-glucoside at dose of 10 mg/kg. The main anti-hyperuricaemic mechanism observed in the extract was uricosuric. Kaempferol-3,4′-β-O-diglucoside was identified as an important component responsible for the total activity of the ethanolic extract and was considered as a good chemical and biological marker of the ethanolic extract of E. sativa. The obtained results indicated the potential of E. sativa in the treatment of hyperuricaemia and its comorbidities.

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“…Sebagai antiinflamasi senyawa kolkisin berperan dalam menghambat kemotaksis sel radang dalam penyembuhan pada radang akibat adanya konsentrasi asam urat yang berlebih dalam darah. Selain itu juga dapat menyebabkan aktivitas enzim XO dalam proses degradasi hipoxantin dan xantin menjadi asam urat terhambat (Murugaiyah and Chan, 2009;Li et al, 2017;Song et al, 2018;Bernardes et al, 2019). Selain itu juga diketahui bahwa senyawa rhombifolin golongan alkaloid diduga dapat berperan sebagai inhibitor enzim XO dan XDH (Xantin dehidrognase), sehingga dapat mencegah hiperurisemia hati secara in vivo.…”

Section: Aktivitas Antihiperurisemiaunclassified

Aktivitas Antihiperurisemia Ekstrak Etanol Daun Sungkai (Peronema canescens Jack) Pada Mencit Putih Jantan

et al. 2021

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Hyperuricemia is a disease caused by an increase in uric acid levels in the blood. Currently, various treatments are developed by utilizing natural ingredients as an anti-hyperuricemia. Sungkai (Peronema canescens Jack) is one of the plants that contain a natural compound that can use as an anti-hyperuricemia treatment is the leaves of sungkai (Peronema canescens Jack.). The aim of this study to determine the anti-hyperuricemia activity of the ethanol extract of Sungkai leaves. The test animals used in this study were male white mice Wistar strain. This study used a completely randomized design (CRD) with 5 groups consisting of negative control (Na CMC 0.5%) positive control (allopurinol 10 mg / kg BW), treatment 1 (extract 125 mg / kg BW), treatment 2 (extract 250 mg / kg BW) and treatment 3 (extract 500 mg / kg BW). The parameters seen were uric acid levels measured using the POCT (Point of Care Test) method and analyzed using One Way Anova statistical analysis and Duncan's continued test. Our results showed that the treatment group with a dose of 125-500 mg / Kg BW reduce uric acid levels in mice. The best dose was a dose of 500 mg / Kg BW in reducing uric acid levels in mice with percent decline of 38.66%.

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In vivo anti-hyperuricemic activity of sesquiterpene lactones from Lychnophora species

Cited by 15 publications

References 24 publications

In vitro inhibition of xanthine oxidase by hydroalcoholic extracts of Corynaea crassa Hook. F

In vitro inhibition of xanthine oxidase by hydroalcoholic extracts of Corynaea crassa Hook. F

Chemical Analysis of Eruca sativa Ethanolic Extract and Its Effects on Hyperuricaemia

Aktivitas Antihiperurisemia Ekstrak Etanol Daun Sungkai (Peronema canescens Jack) Pada Mencit Putih Jantan

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