In vivo Anti-inflammatory Activity of Aqueous Extract of Carthamus caeruleus L Rhizome Against Carrageenan-Induced Inflammation in Mice

Abstract

Carthamus caeruleus L belongs to the family Asteraceae and is reported to have anti-inflammatory, anti-burns and wound healing properties. The aim of the present study was to evaluate the anti-inflammatory activity of aqueous extract of Carthamus caeruleus L rhizome in mice. Carrageenan was used to induce inflammation in mice; the percentage inhibition of edema (% INH), the percentage increase in paw edema volume (% AUG) and histological study were used to evaluate the anti-inflammatory activity. Phytochemical investigations indicate the presence of Carotenoids, flavonoids, terpenoids and steroids, tannins, saponins, coumarins, quinons, mucilage, proteins and glycosides and a very low quantity of phenolic compounds (13.08 ±0.22mg GAE/g extract) and flavonoids (5.02 ±0.55mg QE/g extract) in the rhizome of Carthamus caeruleus L . In acute toxicity test, the oral doses of plant extract administered to mice were not toxic. Our findings show that the treatment with aqueous extract of Carthamus caeruleus L presents a significant inhibitory activity in carrageenan-induced paw inflammation in group A (dose 150 mg / kg) and B (dose 300 mg / kg) compared with the standard drug Diclofenac during the whole period of experimentation; these results were confirmed by the histological study in the paws of mice. Carthamus caeruleus L appears to be a promising plant for further preclinical and clinical trials in inflammation.

Effect of Phenolic Compounds Extracted from Turmeric (Curcuma longa L.) and Ginger (Zingiber officinale) on Cutaneous Wound Healing in Wistar Rats

“…The investigation of anti-inflammatory properties was evaluated by measuring plantar edema induced by 1% carrageenan according to the method described, in [27]. Rats were divided into 9 groups of 7 rats each and separated as follows: negative control rats treated with neutral cream (petroleum jelly: GRIFFON Mark) (NC); positive control rats treated with a pharmaceutical anti-inflammatory product (Diclofenac 1%) (PC); rats treated with a 10% aqueous turmeric-extract-based cream (TAE); rats treated with a 10% ethanolic turmeric-extract-based cream (TEE); rats treated with a 10% methanolic turmeric-extractbased cream (TME); rats treated with a 10% aqueous ginger-extract-based cream (GAE); rats treated with a 10% ethanolic ginger-extract-based cream (GEE); rats treated with a 10% methanolic ginger-extract-based cream (GME).…”

“…The anti-inflammatory effect was assessed by measuring paw volume edema before and after the injection of 1% carrageenan at 0 min, 45 min, 3 h, and 5 h using a digital caliper. Edema extent was assessed by determining the animal's paw volume increase percentage (%PVI) (Equation ( 1)) [27]: Anti-inflammatory activity was evaluated by calculating the edema inhibition percentage (%EIP) according to the following relationship (Equation ( 2)) [27]:…”

Astaxanthin Prevents Hepatosteatosis and Hyperlipidemia but Not Obesity in High-Fat Diet Fed Mice

“…Animal models were proven to be the best fit for studying the bioactivities of compounds such as antifungal, antioxidant, or anti-inflammatory. In particular, induced mice models have recently been established to evaluate these bioactivities before being carried out in clinical trials (Dehimat et al, 2020;Ouda et al, 2021;Shraideh et al, 2020). Therefore, based on the groundwork studies on induced mice models, we applied an obesity-induced mice model with an extremely high-fat diet (60% energy from fat) to assess the hepatosteatosis-preventing ability of AX.…”