In vivo Anti-Inflammatory and Antinociceptive Activity Evaluation of Phenolic Compounds from Sideritis stricta

Abstract

An acetone extract obtained from aerial parts of S. stricta Boiss. & Heldr. apud Bentham, its fractions and phenolic compounds were investigated for their in vivo anti-inflammatory and antinociceptive activities. For the anti-inflammatory activity and for the antinociceptive activity assessment, carrageenan-induced hind paw edema and p-benzoquinone-induced abdominal constriction tests were used, respectively. The acetone extract of the plant and its phenolic fraction exhibited potent inhibitory activity against both bioassay models in mice. From the active phenolic fraction a well-known phenylethanoid glycoside, verbascoside (acteoside) (1), and two flavonoid glycosides, isoscutellarein 7-O-[6‴-O-acetyl-β-D-allopyranosyl-(1→2)]-β-D-glucopyranoside (2) and isoscutellarein 7-O-[6‴-O-acetyl-β-D-allopyranosyl-(1→2)]-6‴-O-acetyl-β-d-glucopyranoside (3), were isolated. During phytochemical studies we also isolated a methoxyflavone, xanthomicrol (4), from the non-polar fraction. The structures of the isolated compounds were established by spectroscopic evidence (UV, IR, 1D- and 2D-NMR, MS). Although antinociceptive and anti-inflammatory activities of the phenolic components were found not significant in the statistical analysis, compounds 1 to 3 showed a notable activity without inducing any apparent acute toxicity as well as gastric damage. Furthermore, a mixture of flavonoid glycosides (2 + 3) exhibited a significant inhibitory effect in both models at a higher dose

Phenolic constituents of Lamium album: Focus on isoscutellarein derivatives

“…Moreover, it is important to highlight that despite the presence of lower amounts of isoscutellarein derivatives as compared to those of phenylethanoid glycosides, these can also be key components on the ethnopharmacological effects of the plant. Indeed, for the last decades, isoscutellarein derivatives have also been described to exert important beneficial activities as antiviral, antioxidant, cytotoxic, antinociceptive, anti-inflammatory and inhibitory activity against osteoclastogenesis (Kupeli, Sahin, Yesilada, Calis, & Ezer, 2007;Nagai, Miyaichi, Tomimori, Suzuki, & Yamada, 1992;Yang et al, 2003;Yoon, Jeong, Hwang, Ryu, & Kim, 2007).…”

Induction of apoptosis by 7-piperazinethylchrysin in HCT-116 human colon cancer cells

“…They have demonstrated anticancer and chemopreventive properties in numerous epidemiological studies (7), and were able to inhibit the proliferation of tumor cells, such as breast, prostate and lung cancer cells, both in vitro and in vivo (8,9), although the exact mechanism is not yet fully understood. The flavonoids are generally safe with low toxicity, making them ideal candidates for cancer chemopreventive agents.…”

Antinociceptive activity of Buddleja globosa (matico) in several models of pain

“…Verbascoside, the major component of EMAT1 and ETMAT1 has been reported for its antinociceptive and anti-inflammatory activity (Küpeli et al, 2007), together with its ability for inhibiting the formation of PGE2, the tumor necrosis factor-␣ (TNF-␣), nitric oxide (NO), suppressing the inducible nitric oxide synthase (iNOS), the enzymatic activity of cyclooxygenase (COX-2) and for exerting a protective action against oxygen free radical-induced peroxidative damage to biomembranes (Lee et al, 2005). Moreover, these not only can explain its anti-inflammatory activity and protective endothelial function but also the analgesic effect (inhibition of PGE2).…”