In vivo anti–nociceptive and anti–inflammatory activities of Lippia alba

Haldar, Sourav; Kar, Biswakanth; Dolai, Narayan; Kumar, Rajesh; Behera, Biswaranjan; Haldar, Pallab Kanti

doi:10.1016/s2222-1808(12)60241-2

Cited by 16 publications

(11 citation statements)

References 5 publications

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“…Because of the great bioactive potential of L. alba, with analgesic, anti-inflammatory, sedative and anti-spasmodic effects, it is greatly used in traditional medicine (Mamun-Or-Rashid et al, 2013;Tavares et al, 2011). Studies have proven that the essential oil of L. alba and its constituents have the following types of activity: antibacterial, antifungal and antiprotozoal (Fabri et al, 2011;Nogueira et al, 2007;Oliveira et al, 2006), antiparasitic (Escobar et al, 2010) and anti-inflammatory (Haldar et al, 2012). In fish subjected to transportation, the essential oil of L. alba has been shown to have anesthetic action and to reduce the levels of oxidative stress (Azambuja et al, 2011, Cunha et al, 2010.…”

Section: Introductionmentioning

confidence: 99%

Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects

Soares

Neves²,

Oliveira

et al. 2016

Aquaculture

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This study investigated the in vivo and in vitro antiparasitic effects of the essential oil of Lippia alba and the bloodrelated and histopathological alterations that it causes in Colossoma macropomum. In the in vitro trial, the anthelminthic effects of 160, 320, 640, 1280 and 2560 mg/L of the essential oil were tested against monogenoideans (Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri) of the gills of this fish, which are its natural parasites. The concentrations of 1280 mg/L and 2560 mg/L showed 100% efficacy after 20 min of exposure to the essential oil, while at lower concentrations this efficacy against the gill monogenoideans only occurred after 2-3 h of in vitro exposure. However, in the controls, mortality of all of these monogenoideans only occurred after 9 h. A total of 240 fry were distributed into four treatments (20 fish per repetition) and three repetitions were used in the in vivo trial for baths with 100 and 150 mg/L of the essential oil of L. alba, for 30 min. The efficacy in this trial against Ichthyophthirius multifiliis in fish exposed to 100 and 150 mg/L of the essential oil was 40.7% and 50.3%, respectively. However, for monogenoideans, there was efficacy of 14.0% only in the fish exposed to 100 mg/L of the essential oil used. Moreover, the fish exposed to these concentrations of the essential oil presented increased plasma glucose levels, thus denoting signs of stress. Severe lesions such as hyperplasia, fusion of the lamellar epithelium, capillary dilatation, epithelial detachment, lamellar aneurysm, epithelial rupture with hemorrhage, congestion, edema, necrosis, mucous cell proliferation, chloride cells and lamellar hypertrophy were observed in the gills of the fish exposed to 100 and 150 mg/L of the essential oil of L. alba. Alterations to total protein levels, hemoglobin, hematocrit, red blood cell number, thrombocytes number, white blood cell number, lymphocytes, eosinophils and blood neutrophil number was also observed in these fish. The essential oil of L. alba showed great potential for antiparasitic treatment, given that it had high in vitro efficacy against monogenoideans and in vivo efficacy against the protozoon I. multifiliis. Because of the low concentrations of the essential oil (100 and 150 mg/L) that were tolerated by the fish and thus could be used in the therapeutic baths, the efficacy against monogenoideans was low. This indicates that there is a need for additional strategies for using this essential oil in antiparasitic treatments, since the concentrations that eliminate these ectoparasites are toxic for the hosts. Lastly, this was the first study on the antiparasitic activity of L. alba. Statement of Relevance The manuscript entitled "Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects", represents original article on use of the essential oil of Lippia alba on ectoparasites of tambaqui, an important finfish of Amazon regio...

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Section: Introductionmentioning

confidence: 99%

Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects

Soares

Neves²,

Oliveira

et al. 2016

Aquaculture

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“…The leaves are used for flavoring agents [15] such as mole sauces from Oaxaca, Mexico [16]. The plant is used traditionally for colds, sedative, somatic, cough, antidepressant, dysentery, analgesic, febrifuge and gastrointestinal ailments [17,18]. A review of the literature showed that L. alba has been found to have a wide range of activities, including antioxidant, antiulcer, antibacterial, antifungal, antinociceptive, antiviral, anti-inflammatory, antiprotozoal, cytotoxic, as well as neurosedative activities [18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

“…The plant is used traditionally for colds, sedative, somatic, cough, antidepressant, dysentery, analgesic, febrifuge and gastrointestinal ailments [17,18]. A review of the literature showed that L. alba has been found to have a wide range of activities, including antioxidant, antiulcer, antibacterial, antifungal, antinociceptive, antiviral, anti-inflammatory, antiprotozoal, cytotoxic, as well as neurosedative activities [18][19][20][21]. Many volatile and non-volatile components such as neral, geranial, E-caryophyllene, caryophyllene oxide, allo-aromadendrene, theveside, 8epiloganin, geniposide, mussaenoside, apigenin-7-O-glucuronide, luteoline-7-O-glucuronide etc.…”

Section: Introductionmentioning

confidence: 99%

In vivo and in silico evaluation of analgesic activity of Lippia alba

Aziz¹,

Mehedi

Akter

et al. 2019

Clin Phytosci

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Background: This study was conducted to evaluate the analgesic activity of different extracts of Lippia alba (L. alba) along with in silico evaluation of analgesic activity of the isolated compounds from L. alba against cyclooxygenase-2 enzyme and ADME/T analysis of isolated compounds. Method: In vivo analgesic activity of different extracts of L. alba was evaluated by acetic acid-induced writhing, tail immersion and hot plate on Swiss albino mice of either sex. In silico activity of the isolated compounds and ADME/T analysis were performed by Schrödinger-Maestro (Version 10.1) and OSIRIS Data warrior (version 4.6.1) softwares. Results: Three different extracts (Methanolic extract: ME; Petroleum ether extract: PEE; Dichloromethane extract: DCME) of 250 mg/kg and 500 mg/kg doses were used in the experiments to evaluate analgesic activity. In acetic acid-induced writhing test, significant results were seen for PEE (500 mg/kg) and DCME (500 mg/kg), which were 53.09 ± 2.87 & 50.09 ± 4.24%, respectively. In tail immersion test, the best latency time was found at + 60 min for PEE (500 mg/kg) which is (5.65 ± 0.25) sec. For hot plate test, DCME at a dose 500 mg/kg showed the highest increase in latency time, which was 13.48 ± 0.33 s. In the case of in silico evaluation of analgesic activity, the compounds such as geranial, neral, (E)caryophyllene, caryophyllene oxide, mussaenide, and 8-epi-loganin meet the condition of Lipinski's rule of five. Among these safe compounds, 8-epi-loganin showed the best docking score of − 8.17 kcal/mol against cyclooxygenase-2 enzyme (PDB ID: 6COX), which was almost similar to that of the standard drug, Celecoxib (− 11.11 kcal/mol). Conclusion: In conclusion, L. alba can be a potent source of analgesic medicine and further modification and simulation studies are required to establish the effectiveness of 8-epi-loganin.

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“…A ação antinociceptiva também deu destaque à FA, que promoveu latência estatisticamente constante (3,03-3,69 seg), mas significativa em comparação com controle negativo, quando avaliada 30, 60, 120, 180 e 240min após a administração oral da FA por meio do método de imersão de calda. Segundo os autores, tais atividades foram possíveis devido à alta concentração de flavonoides no extrato, conhecidos pela diminuição da síntese de prostaglandinas (HALDAR et al, 2012;SIQUEIRA-LIMA et al, 2019).…”

Section: Neuroativa E Anti-inflamatóriaunclassified