In vivo anti-tumor effect of dual release of cisplatin and adriamycin from biodegradable gelatin hydrogel

Konishi, Mitsunaga; Tabata, Yasuhiko; Kariya, Masatoshi; Hosseinkhani, Hossein; Suzuki, Ayako; Fukuhara, Ken; Mandai, Masaki; Takakura, Kenji; Fujii, Shingo

doi:10.1016/j.jconrel.2004.11.014

Cited by 143 publications

(83 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The intratumor administration of a sustained-release antitumor drug system to tumor-bearing mice 33,34 showed significantly greater antitumor effect on the tumor growth inhibition ratio and on the survival period when compared to drug solutions alone. The antitumor efficacy of 5-FU-loaded microspheres was studied in tumor-bearing rats after injection of C6 malignant glioma cells in the brain, and this study showed a significant decrease in the mortality of rats treated with 5-FU-loaded microspheres when compared with the group injected with 5-FU solution.…”

Section: Release Of 5-fu In the In Vivo Rat Studymentioning

confidence: 97%

Hemostatic absorbable gelatin sponge loaded with 5-fluorouracil for treatment of tumors

Sun¹,

Chen²,

Yuan³

2013

IJN

View full text Add to dashboard Cite

Background: Surgical tumor resection is the main treatment for tumors however the treatment process often results in massive bleeding and tumor cell residue. The main aim of this research was to address problems such as bleeding, systemic chemotherapy side effects while enhancing quality of life, and increasing drug concentrations at the tumor site by developing a novel formulation with local long-term efficacy for treatment of tumors and to stop bleeding. Methods: 5-Fluorouracil (5-FU) was suspended in an ethyl acetate solution of poly D,Llactide-co-glycolic acid (PLGA) and a vacuum drying method was applied. The hemostatic gelatin sponge loaded with 5-FU was prepared by absorption of the suspension. The in vitro and in vivo characteristics of the hemostatic gelatin sponge loaded with 5-FU (5-FU-HAGS) were investigated. Results: 5-FU-HAGS (hemostatic absorbable gelatin sponge loaded with 5-fluorouracil) was successfully produced with controlled release of the content and was reproducibly suitable for local tumor treatment as an implant to stop bleeding. The encapsulation efficiency of 5-FU-HAGS was above 98%. The in vitro 5-FU release kinetic profile matched a near zero-order equation for 20 days. The in vivo 5-FU plasma concentration was at a more stable level than when 5-FU solution was administered by subcutaneous injection. Bleeding can be stopped more effectively by coating a piece of blank gelatin sponge. The survival ratio of tumor-bearing mice using a 5-FU-HAGS subcutaneous implant was higher when compared to mice given a subcutaneous injection of 5-FU solution. Conclusion:The 5-FU-HAGS system is a potential and effective way of enhancing the survival ratio and improving the quality of life of tumor-bearing mice.

show abstract

Section: Release Of 5-fu In the In Vivo Rat Studymentioning

confidence: 97%

Hemostatic absorbable gelatin sponge loaded with 5-fluorouracil for treatment of tumors

Sun¹,

Chen²,

Yuan³

2013

IJN

View full text Add to dashboard Cite

show abstract

“…The intra-tumor administration of the drug delivery system to mice (43,44) had an enhanced antitumor effect. The antitumor efficacy of the microsphere delivery system was also studied in rats which indicated much more significantly reduction in the mortality rate than the group administered with the water solution (45).…”

Section: -Fu In Vitro Release Profile and Encapsulation Efficiencymentioning

confidence: 99%

Development of a 5-fluorouracil-loaded PLGA microsphere delivery system by a solid-in-oil-in-hydrophilic oil (S/O/hO) novel method for the treatment of tumors

et al. 2014

View full text Add to dashboard Cite

Abstract. Tumor treatment requires a long-term regimen of chemotherapy, and both surgical tumor resection and radiation therapy are also used. The present study aimed to develop a novel method for 5-fluorouracil (5-FU)-loaded microspheres which enhance the therapeutic effects of chemotherapy, the quality of life of patients and reduce chemotherapy systemic side-effects. The preparation of a 5-FU microsphere delivery system by a solid-in-oil-in-hydrophilic oil (S/O/hO) novel method was carried out and then in vitro and in vivo evaluation of the 5-FU-microsphere delivery system was conducted. The 5-FU microsphere delivery system prepared had sustainedrelease function and achieved local treatment efficacy for tumors. The encapsulation efficiency of the 5-FU microsphere delivery system was >90% [better than the fabrication method using water-in-oil-in-water (W/O/W)]. The drug release profile from the 5-FU-loaded sustained-release microsphere delivery system matched the pseudo zero-order equation for 30 days in vitro. The plasma concentration of 5-FU was higher than the water solution by subcutaneous injection. The tumor growth rate of rabbits using the 5-FU microsphere delivery system was much lower than the rate in rabbit using a subcutaneous injection of 5-FU water solution. The 5-FU-loaded sustainedrelease microspheres using the novel method (S/O/hO) is a potential and effective method with which to inhibit tumor growth.Introduction 5-Fluorouracil (5-FU) is one of the classical drugs for tumor chemotherapy (1,2). It has been used for colorectal tumors (3-6), pancreatic cancer (5,6), actinic keratosis (7,8) and breast tumors (9-12). As a systemic injection of 5-FU often leads to systemic toxic side-effects, tumor in situ sustained-release microspheres or a hydrogel delivery system will certainly improve the antitumor effects of the drug (13,14). Different delivery systems for 5-FU have been developed, including microsphere delivery systems (15), nanospheres (16), 5-FU microspheres for brain tumor therapy (17), 5-FU microspheres for malignant glioma therapy (18), 5-FU hydrogels for drug administration in vivo (19), matrix microspheres containing a 5-FU coat using Eudragit S100 (20), and nanoscale-particles for topical delivery (21), to name just a few (22-29). The specific delivery system can reduce the systemic toxicity and prevent anticancer drug degradation (30-33). However, different methods have different weaknesses such as low encapsulation efficiency, burst release or insufficient duration for drug release.We previously developed various preparation methods for biological molecular protein drug delivery such as the waterin-oil-in-hydrophilic oil-in-water (W/O/hO/W) method (34), solid-in-oil-in-oil-in-water (S/O/O/W) method (35-37), and solid-in-oil-in-hydrophilic oil-in-ethanol (S/O/hO/E) method (38,39,40). The chemical agents were not microencapsulated in the microspheres using these preparation methods. The present study aimed to develop a 5-FU-loaded sustained-release microsphere system using a novel meth...

show abstract

“…There has been a great deal of interest in the use of hydrogels as chemotherapeutic drug delivery systems for drugs including paclitaxel, doxorubicin, DTX, tamoxifen, and cisplatin. [162][163][164][165][166][167][168][169][170][171] Furthermore, studies have revealed that strategies are required to overcome the disadvantages of chemotherapeutic drugs such as cisplatin, which is usually intravenously administered, whereby 90% becomes linked to hemoproteins and 10% is free to enter into the cells.…”

Section: Hydrogelsmentioning

confidence: 99%

Nanotechnology-based drug delivery systems for treatment of oral cancer: a review

Calixto¹,

Fonseca-Santos²,

Chorilli³

et al. 2014

IJN

151

View full text Add to dashboard Cite

Oral cancer (oral cavity and oropharynx) is a common and aggressive cancer that invades local tissue, can cause metastasis, and has a high mortality rate. Conventional treatment strategies, such as surgery and chemoradiotherapy, have improved over the past few decades; however, they remain far from optimal. Currently, cancer research is focused on improving cancer diagnosis and treatment methods (oral cavity and oropharynx) nanotechnology, which involves the design, characterization, production, and application of nanoscale drug delivery systems. In medicine, nanotechnologies, such as polymeric nanoparticles, solid lipid nanoparticles, nanostructured lipid carriers, gold nanoparticles, hydrogels, cyclodextrin complexes, and liquid crystals, are promising tools for diagnostic probes and therapeutic devices. The objective of this study is to present a systematic review of nanotechnology-based drug delivery systems for oral cancers.

show abstract

In vivo anti-tumor effect of dual release of cisplatin and adriamycin from biodegradable gelatin hydrogel

Cited by 143 publications

References 37 publications

Hemostatic absorbable gelatin sponge loaded with 5-fluorouracil for treatment of tumors

Hemostatic absorbable gelatin sponge loaded with 5-fluorouracil for treatment of tumors

Development of a 5-fluorouracil-loaded PLGA microsphere delivery system by a solid-in-oil-in-hydrophilic oil (S/O/hO) novel method for the treatment of tumors

Nanotechnology-based drug delivery systems for treatment of oral cancer: a review

Contact Info

Product

Resources

About