In vivo anticancer activity of lysates from Trypanosoma cruzi of different genetic groups

Batmonkh, Zetsegsaikhan; Kallinikova, V. D.; Pakhorukova, L. V.; Кравцов, Э. Г.; Karpenko, L. P.; Далин, М. В.

doi:10.1007/s10517-006-0395-6

Cited by 16 publications

(6 citation statements)

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“…However, in previous studies, Darani et al (2009) claimed that the crude antigens of T. canis eggs showed antitumor potential. The anti-cancer potential of parasitic products is mostly investigated in crude form; a study by Batmonkh et al (2006) looked at the antitumor properties of parasites ( Trypanosoma cruzi ) in a mouse model in which parasite lysates were able to reduce the tumor size compared to the control group. The present study focused on a specific part of T. canis ES proteins that do not interpret the results as misleading.…”

Section: Discussionmentioning

confidence: 99%

In vitro anti-gastrointestinal cancer activity of Toxocara canis-derived peptide: Analyzing the expression level of factors related to cell proliferation and tumor growth

et al. 2022

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Background: Recently, a hypothesis about the negative relationship between cancers and parasites has been proposed and investigated; some parasitic worms and their products can affect the cancer cell proliferation. Due to the potential anti-cancer effect of helminthic parasites, in the present study, the excretory–secretory protein of Toxocara canis (T. canis) parasite was used to evaluate the possible anti-cancer properties and their effect on gastrointestinal and liver cancer cell proliferation-related genes in laboratory conditions.Methods and materials: The selected synthesized peptide fraction from the T. canis excretory–secretory Troponin protein peptide (ES TPP) was exposed at 32, 64, 128, and 256 μg/ml concentrations to three gastrointestinal cancer cell lines AGS, HT-29, and Caco 2, as well as HDF cells as normal cell lines. We used the MTT assay to evaluate cellular changes and cell viability (CV). Variations in gene (Bcl-2, APAF1, ZEB1, VEGF, cyclin-D1, and caspase-3) expression were analyzed by real-time RT-PCR.Results: After 24 h of exposure to pept1ides and cell lines, a decrease in CV was observed at a concentration of 64 μg/ml and compared to the control group. Then, after 48 h, a significant decrease in the CV of Caco 2 cells was observed at a concentration of 32 μg/ml; in the other cancer cell lines, concentrations above 32 μg/ml were effective. The peptide was able to significantly alter the expression of the studied genes at a concentration of 100 μg/ml.Conclusion: Although the studied peptide at high concentrations could have a statistically significant effect on cancer cells, it is still far from the standard drug and can be optimized and promising in future studies.

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Section: Discussionmentioning

confidence: 99%

In vitro anti-gastrointestinal cancer activity of Toxocara canis-derived peptide: Analyzing the expression level of factors related to cell proliferation and tumor growth

et al. 2022

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“…As early as 1948, researchers injected T. cruzi endotoxin directly into tumor-bearing mice, successfully inhibiting the growth of various types of tumors ( Hauschka and Goodwin, 1948 ). Studies involving the lysates of different genetic groups of T. cruzi strains have revealed their notable anti-tumor capabilities ( Sheklakova et al., 2003 ; Batmonkh et al., 2006 ). However, the significant side effects associated with these treatments hinder their direct use in cancer therapy.…”

Section: T Cruzimentioning

confidence: 99%

A novel enemy of cancer: recent investigations into protozoan anti-tumor properties

Zheng,

Lu,

Zhou

et al. 2024

Front. Cell. Infect. Microbiol.

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Cancer remains a significant global health issue, despite advances in screening and treatment. While existing tumor treatment protocols such as surgery, chemotherapy, radiotherapy, targeted therapy, and immunotherapy have proven effective in enhancing the prognosis for some patients, these treatments do not benefit all patients. Consequently, certain types of cancer continue to exhibit a relatively low 5-year survival rate. Therefore, the pursuit of novel tumor intervention strategies may help improve the current effectiveness of tumor treatment. Over the past few decades, numerous species of protozoa and their components have exhibited anti-tumor potential via immune and non-immune mechanisms. This discovery introduces a new research direction for the development of new and effective cancer treatments. Through in vitro experiments and studies involving tumor-bearing mice, the anti-tumor ability of Toxoplasma gondii, Plasmodium, Trypanosoma cruzi, and other protozoa have unveiled diverse mechanisms by which protozoa combat cancer, demonstrating encouraging prospects for their application. In this review, we summarize the anti-tumor ability and anti-tumor mechanisms of various protozoa and explore the potential for their clinical development and application.

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“…In this complex scenario, where evidence points to both antineoplastic and pro-tumorigenic properties of T. cruzi, it is evident that the interaction of the parasite, its molecules, the host, and cancer involves diverse mechanisms that operate differentially depending on the observational context. The outcome of this intricate interaction depends on various factors: 1) the type of tumor, 2) the virulence and phenotypic characteristics of the T. cruzi strain involved-for instance, some clones exhibit antitumor effects only during infection or immunization, while others exhibit a delayed effect- (Batmonkh et al, 2006), 3) whether the immunogens are parasite lysates or live parasites, and 4) in the case of live parasites, the stage of the disease-acute or chronic phase-regardless of the infected host species.…”

Section: T Cruzi Chagas Disease and Cancer: The Paradox Unveiledmentioning

confidence: 99%

Trypanosoma cruzi, Chagas disease and cancer: putting together the pieces of a complex puzzle

Kaufman,

Farré,

Biscari

et al. 2023

Front. Cell Dev. Biol.

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Considering the extensive and widespread impact on individuals, cancer can presently be categorized as a pandemic. In many instances, the development of tumors has been linked to endemic microbe infections. Among parasitic infections, Trypanosoma cruzi stands out as one of the most extensively discussed protozoans in the literature that explores the association between diseases of parasite origin and cancer. However, the effective association remains an unsolved paradox. Both the parasite, along with protozoan-derived molecules, and the associated antiparasitic immune response can induce alterations in various host cell pathways, leading to modifications in cell cycle, metabolism, glycosylation, DNA mutations, or changes in neuronal signaling. Furthermore, the presence of the parasite can trigger cell death or a senescent phenotype and modulate the immune system, the metastatic cascade, and the formation of new blood vessels. The interaction among the parasite (and its molecules), the host, and cancer undoubtedly encompasses various mechanisms that operate differentially depending on the context. Remarkably, contrary to expectations, the evidence tilts the balance toward inhibiting tumor growth or resisting tumor development. This effect is primarily observed in malignant cells, rather than normal cells, indicating a selective or specific component. Nevertheless, nonspecific bystander mechanisms, such as T. cruzi’s adjuvancy or the presence of proinflammatory cytokines, may also play a significant role in this phenomenon. This work aims to elucidate this complex scenario by synthesizing the main findings presented in the literature and by proposing new questions and answers, thereby adding pieces to this challenging puzzle.

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In vivo anticancer activity of lysates from Trypanosoma cruzi of different genetic groups

Cited by 16 publications

References 1 publication

In vitro anti-gastrointestinal cancer activity of Toxocara canis-derived peptide: Analyzing the expression level of factors related to cell proliferation and tumor growth

In vitro anti-gastrointestinal cancer activity of Toxocara canis-derived peptide: Analyzing the expression level of factors related to cell proliferation and tumor growth

A novel enemy of cancer: recent investigations into protozoan anti-tumor properties

Trypanosoma cruzi, Chagas disease and cancer: putting together the pieces of a complex puzzle

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