In vivo antifungal activity and biocompatibility of Cryptocarya moschata

Zoccolotti, Jacqueline de Oliveira; Cavalheiro, Alberto José; Tasso, Camilla Olga; Oliveira, Caroline Correa de; Ribas, Beatriz Ribeiro; Ferrisse, Túlio Morandin; Costa, Carlos Alberto de Souza; Jorge, Janaína Habib

doi:10.1007/s00203-022-03177-1

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…6 In recent years, many medicinal plants are researched for their antimicrobial properties against denture infections. 7 One such is Vaccinium macrocarpon, commonly called as cranberry has shown promising antiinfective potential owing to its high and enriched phytochemical profile. 8 In medical health sciences, it is commonly used for treatment of urinary tract infections.…”

Section: Introductionmentioning

confidence: 99%

Effect Of Vaccinium Macrocarpon On Candida Albicans Adhesion To Denture Base Resin

MDS¹

2022

Journal of Pharmaceutical Negative Results

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Objective This study aimed to assess the effectivenessof Vaccinium macrocarpon (cranberry)extract on Candida albicans adhesion tocranberry added denture base biomaterial. Method Different proportions of cranberryextract were incorporated into polymer of polymethyl methacrylate (PMMA) self - cure resin material forfabricationfifty of 4 mm x 4mm x 2mm samples. Group I contained 0 wt/wt% cranberry was used as control group. 0.5wt/wt%, 1.0 wt/wt%, 1.5 wt/wt% and 2 wt/wt% was graded as Group II to V in order.Addition of 0.1mL of Candida albicans suspension was done in each well of tissue culture plate and incubated for 48 hours. 2% cranberrywasused as disinfectant solution in four serial dilutions at undiluted, 1:10, 1:100, 1:1000 ratio. Immersion of resin samples in disinfectant solution was done for 10 minutes. A colony forming unit was measuredafter 0.1mL of the solution was plated on as sabouraud dextrose agar (SDA) plate and cultured for 72 hours. Data were subjected to statistical analysis. Results The mean colony forming units (CFU) per mL showed that there were severalconfluent colonieswith all proportions of cranberry extract added poly methyl methacrylate (CA-PMMA) resin samplesin undiluted and 1:10 dilution state with both distilled water and cranberry disinfectants. While immersion indistilled waterat 1 :100 dilution, mean CFU/mL was 2.99 0.29in all groups of CA-PMMA. In 1 :1000 dilution state, the mean SD was 117 0.42.Immersion in cranberry solution at 1:100 and 1:1000 showed 2.65 0.66 and 1.26 0.59 for all groups of CA-PMMA. On comparison between distilled water and cranberry as disinfectants, a statistically significant difference was noted with all groups as p < 0.043. Insignificant variation was observed within the groups with both disinfectants as p > 0.05. Conclusion Adherence of Candida albicans to cranberry added self-cure poly methyl methacrylate biomaterial was evidenced to be less irrespective of extract proportions of 1%, 1.5% and 2% in PMMA.Cranberry’s effectiveness as disinfectant showed lesscandida growth when compared to distilled water immersion. This infers that cranberry has anti-adherence property when used both as a polymer constituent material as well as disinfectant solution.

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Section: Introductionmentioning

confidence: 99%

Effect Of Vaccinium Macrocarpon On Candida Albicans Adhesion To Denture Base Resin

MDS¹

2022

Journal of Pharmaceutical Negative Results

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Cryptocarya moschata fractions decrease planktonic cells and biofilms of Candida albicans and Streptococcus mutans

Oliveira,

Ribas,

Ferro

et al. 2024

Biofouling

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Cryptocarya moschata extract decreases single and mixed biofilms on acrylic resins

Tasso,

Ribas,

Ferrisse

et al. 2024

Oral Diseases

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ObjectiveThis study proposed to assess the effect of Cryptocarya moschata extract on single and mixed biofilms formed on denture base and reline acrylic resin.Materials and MethodsSingle and mixed biofilms of Candida albicans and Streptococcus mutans were formed on the samples and treated with C. moschata extract; Nystatin solution at 100,000 IU/mL or Penicillin antibiotic solution at 100,000 IU/mL; or PBS solution. Antimicrobial activity was analyzed by counting colony‐forming units, metabolism assay, assessment of protein components of the biofilm matrix, and of cell viability using confocal laser scanning microscopy (CLSM). Data were submitted to ANOVA and Tukey's post‐test (α = 0.05).ResultsCryptocarya moschata extract reduced cell viability of C. albicans and S. mutans single and mixed biofilms formed on samples. For all types of biofilms in the C. moschata group, there was a log reduction of the biofilm, proven by the Alamar Blue assay. Analyzing the extracellular matrix protein components, groups treated with the extract exhibited a lower level of fluorescence compared to the PBS groups. Reduction in thickness biofilm and viable cells was perceptible in the C. moschata group when assessing through CLSM.ConclusionCryptocarya moschata extract reduced the single and mixed biofilms of C. albicans and S. mutans on acrylic resins.

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In vivo antifungal activity and biocompatibility of Cryptocarya moschata

Cited by 3 publications

References 27 publications

Effect Of Vaccinium Macrocarpon On Candida Albicans Adhesion To Denture Base Resin

Effect Of Vaccinium Macrocarpon On Candida Albicans Adhesion To Denture Base Resin

Cryptocarya moschata fractions decrease planktonic cells and biofilms of Candida albicans and Streptococcus mutans

Cryptocarya moschata extract decreases single and mixed biofilms on acrylic resins

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