In-vivo antimalarial activity of<i>Cassia occidentalism Morinda morindoides</i>and<i>Phyllanthus niruri</i>

Tona, L.; Mesia, K.; Ngimbi, N. P.; Chrimwami, B; Okond'ahoka,; Cimanga, K.; Bruyne, Tess De; Apers, Sandra; Hermans, Nina; Totté, J.; Pieters, Luc; Vlietinck, A. J.

doi:10.1080/00034983.2001.11813614

Cited by 124 publications

(85 citation statements)

References 36 publications

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“…Chloroquine (25 mg/kg) and quinine (20 mg/kg) were used as positive controls; saline was used as negative control. The method was previously described (Tona et al, 2001). Ethanolic extracts were prepared as previously described for other extracts.…”

Section: In Vivo Antimalarial Activitymentioning

confidence: 99%

In vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine

Lusakibanza

Mesia

Tona

et al. 2010

Journal of Ethnopharmacology

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Aim of the studyThe in vitro antiplasmodial activity and cytotoxicity of methanolic and dichloromethane extracts from five Congolese plants were evaluated. The plants were selected following an ethnobotanical survey conducted in D.R. Congo and focusing on plants used traditionally to treat malaria. The in vivo antimalarial activity of aqueous and methanolic extracts active in vitro was also determined in mice infected by Plasmodium berghei berghei. Materials and methodsThe growth inhibition of Plasmodium falciparum strains was evaluated using the measurement of lactate dehydrogenase activity. The extracts (aqueous, CH 3 OH, EtOH and CH 2 Cl 2 ) were prepared by maceration and tested in vitro against the 3D7 (chloroquine sensitive) and W2 (chloroquine resistant) strains of Plasmodium falciparum and against the human normal fetal lung fibroblasts WI-38 to determine the selectivity index. Some extracts were also used at the dose of 300mg/kg to evaluate their activity in mice infected since 4 days by Plasmodium berghei. ResultsTwo plants presented a very high activity (IC 50 < 3µg/ml). These plants were Strychnos icaja roots bark (MeOH and CH 2 Cl 2 ) and Physalis angulata leaves (MeOH and CH 2 Cl 2 ). One plant (Anisopappus chinensis whole plant, MeOH and CH 2 Cl 2 ) presented a high activity (IC50 < 15µg/ml).The extracts of Anisopappus chinensis and Physalis angulata showed also a good inhibition of parasitemia in vivo. Flavonoids, phenolic acids and terpenes were identified in these plants by a general phytochemical screening method. ConclusionThree plants showed a very interesting antiplasmodial activity (Anisopappus chinensis, Physalis angulata, Strychnos icaja) and one of them showed a good selectivity index ( >10, Anisopappus chinensis). Anisopappus chinensis and Physalis angulata were also active in vivo.

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Section: In Vivo Antimalarial Activitymentioning

confidence: 99%

In vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine

Lusakibanza

Mesia

Tona

et al. 2010

Journal of Ethnopharmacology

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“…In vivo acute toxicity was determined in mice using standard procedures (Lorke, 1983;Tona and Mesia, 2001). Groups of five starved animals were given increasing dosages of test sample (250, 500, 750, 1000, 1500 and 5000 mg/kg body weight) orally and the number of deaths occurring within 24-48 h noted.…”

Section: Acute Toxicitymentioning

confidence: 99%

The in vitro anti-plasmodial and in vivo anti-malarial efficacy of combinations of some medicinal plants used traditionally for treatment of malaria by the Meru community in Kenya

Gathirwa

Rukunga

Njagi

et al. 2008

Journal of Ethnopharmacology

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The use of herbal drugs as combinations has existed for centuries in several cultural systems. However, the safety and efficacy of such combinations have not been validated. In this study, the toxicity, anti-plasmodial and antimalarial efficacy of several herbal drug combinations were investigated. Lannea schweinfurthii, Turraea robusta and Sclerocarya birrea, used by traditional health practitioners in Meru community, were tested for in vitro anti-plasmodial and in vivo anti-malarial activity singly against Plasmodium falciparum and Plasmodium berghei, respectively. Methanolic extract of Turraea robusta was the most active against Plasmodium falciparum D6 strain. Aqueous extracts of Lannea schweinfurthii had the highest anti-plamodial activity followed by Turraea robusta and Sclerocarya birrea. D6 was more sensitive to the plant extracts than W2 strain. Lannea schweinfurthii extracts had the highest anti-malarial activity in mice followed by Turraea robusta and Sclerocarya birrea with the methanol extracts being more active than aqueous ones. Combinations of aqueous extracts of the three plants and two others (Boscia salicifolia and Rhus natalensis) previously shown to exhibit anti-plasmodial and anti-malarial activity singly were tested in mice. Marked synergy and additive interactions were observed when combinations of the drugs were assayed in vitro. Different combinations of Turraea robusta and Lannea schweinfurthii exhibited good in vitro synergistic interactions. Combinations of Boscia salicifolia and Sclerocarya birrea; Rhus natalensis and Turraea robusta; Rhus natalensis and Boscia salicifolia; Turraea robusta and Sclerocarya birrea; and Lannea schweinfurthii and Boscia salicifolia exhibited high malaria parasite suppression (chemo-suppression >90%) in vivo when tested in mice. The findings are a preliminary demonstration of the usefulness of combining several plants in herbal drugs, as a normal practice of traditional health practitioners.

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“…It is one of the most killer diseases worldwide with 90% of the death recorded in Sub-Saharan Africa (Mccombie, 2002). Control of the disease mostly relies on drug therapies but most currently available antimalaria drugs have been used for decades with the rapid emergence of widespread drug resistance (Tona et al, 2001;Tolu et al, 2007;Ene et al, 2009). In spite of the socio economic problem and health hazard associated with poor treatment and management of malarial infections, most people in rural and urban area in Southwest Nigeria still relies on medicinal plants for the management and treatment of the disease.…”

Section: Introductionmentioning

confidence: 99%

In vivo anti-malaria activity of methanolic leaf and root extracts of Sphenocentrum jollyanum Pierre

Olorunnisola¹

2011

Afr. J. Pharm. Pharmacol.

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Methanolic leaf and root extracts of Sphenocentrum jollyanum Pierre were tested in vivo for antimalarial activity in Swiss albino mice inoculated with chloroquine resistant Plasmodium berghei NK 67 strain. The two extracts exhibited a significant (p>0.05) dose dependant anti-plasmodial activities in isolation and when combined in the 4-days curative standard test with a high mean of survival time. Although, the standard drug, Arthemether -lumefartrin, showed the highest antimalaria potency (81.4%), those of the leaf (74.4%) and root extracts (54.1%) in isolation and combination (63.4%) compared favorably. The leaf extract demonstrated higher antimalarial potency than the root or combination. The two extracts also produced a significant (p>0.05) positive effect on the weight and hematology values in the treated animals. Phytochemical screening of the plant (leaf and the root extracts) reveals the presences of flavonoids, steroids, terpenoids, tannins and alkaloids. The antiplasmodia activity of these plant extract might be attributed to these phytochemicals compounds. The results showed that the leaf and root of S. jollyanum possesses an anti-malarial activity which was effective against chloroquine resistance strain. This work has validated the traditional uses of S. jollyanum root extract in the treatment of malaria and also reported for the first time the anti-malarial property of the S. jollyanun leaf extract.

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In-vivo antimalarial activity ofCassia occidentalism Morinda morindoidesandPhyllanthus niruri

Cited by 124 publications

References 36 publications

In vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine

In vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine

The in vitro anti-plasmodial and in vivo anti-malarial efficacy of combinations of some medicinal plants used traditionally for treatment of malaria by the Meru community in Kenya

In vivo anti-malaria activity of methanolic leaf and root extracts of Sphenocentrum jollyanum Pierre

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