In Vivo Antiplasmodial Activity and Toxicological Analyses of the Ethanolic Leaf and Twig Extract of Faurea speciosa Welw. (Proteaceae)

Abstract

In Africa, medicinal plants are commonly used to treat malaria and other diseased conditions. The ethanolic leaf and twig extract of Faurea speciosa has been shown to possess promising antiplasmodial properties. This present study was aimed at investigating its antiplasmodial effect in vivo. Qualitative phytochemical screening was carried out on the plant samples using standard methods. The antiplasmodial effect against early infection, curative effect against established infection, and prophylactic effect against residual infection were studied in vivo in Plasmodium berghei-infected mice while the carrageenan-induced edema model in chicks was used for anti-inflammatory tests. The phosphomolybdenum and DPPH radical scavenging assays were used in the evaluation of antioxidant potential. Acute toxicity of the extract was evaluated using the Organization for Economic Cooperation and Development (OECD) guidelines. Phytochemical screening of plant samples revealed the presence of flavonoids, coumarins, tannins, saponins, and glycosides. Faurea speciosa leaf and twig extract exhibited significant antiplasmodial activities in the mouse model with parasite suppression rates of 66.63%, 71.70%, and 56.93% in the suppressive, curative, and prophylactic tests, respectively. A 55.50% reduction of edema in the anti-inflammatory test indicated moderate success in reducing inflammation. The total antioxidant capacity of the extract was determined to be 65.4 mg AAE/g of extract, while in the DPPH radical scavenging assay, the IC50 value was found to be 499.4 μg/mL. With the exception of an inconsistent rise in urea level, there was no significant difference in the other biochemistry parameters in the acute toxicity studied. The median lethal dose (LD50) of the extract was over 2000 mg/kg. The results of this study show that Faurea speciosa leaf and twig extract has promising antimalarial capabilities and is fairly safe at low concentrations.

Antimalarial Efficacy of Hydromethanolic Root Extract and Solvent Fractions of Urtica simensis Hochst. ex. A. Rich. (Urticaceae): An Experimental Study on Plasmodium berghei-Infected Mice

“…Furthermore, the parasite inhibition displayed by 80% methanol extracts is equivalent to that observed in previous experiments using methanolic soybean seed extract ( Glycine max ) [ 53 ], ethanolic leaf and twig extract of Faurea speciosa [ 54 ], 80% methanolic root extract of Echinops hoehnelii [ 55 ], hydroethanolic stem bark extract of Myrianthus libericus [ 40 ], and aqueous fraction of Schinus molle seeds [ 56 ]. Nevertheless, the chemosuppression demonstrated by aqueous extract is less than that observed in a study using an aqueous extract of Schinus molle seeds [ 56 ].…”

Allosteric Modulation of the Main Protease (MPro) of SARS-CoV-2 by Casticin—Insights from Molecular Dynamics Simulations

“…Herbal remedies have existed in folkloric medicine for a long time [17][18][19]. Some of the plants used in such herbal remedies provide elegant structural scaffolds that can serve as lead compounds in drug discovery efforts.…”

In vivo hepatoprotective and nephroprotective effects ofStenocarpus sinuatusleaf extract against ifosfamide‐induced toxicity in rats

“…once daily for 6 days and served as negative control. Groups 3, 4, and 5 were given oral SSLE suspended in 0.5% CMC in distilled water in doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg, [ 86 ] respectively, once daily for 6 days. 2 h later, groups 2‐5 were administered IFOS (40 mg/mL) in a dose of 50 mg/kg/day, i.p.…”