In Vivo Antiplasmodial Activity of Entandrophragma cylindricum (Sprague) Sprague Ethyl Acetate Extract in Plasmodium berghei-Infected Mice

Nadia, Noumedem Anangmo Christelle; Cédric, Yamssi; Christian, Ngongang Ouankou; Njonnou, Sylvain Raoul Simeni; Jeanette, Yondo; Azizi, Mounvera Abdel; Foka, Eric Igor Sop; Diane, Djape Guangue; Abass, Chahdini Gbambie; Gabriel, Tsila Henri; Mbida, Mpoame

doi:10.1155/2020/8846067

Cited by 5 publications

(4 citation statements)

References 16 publications

(13 reference statements)

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“…The antiplasmodial activities of the extracts could be attributed to different effects; individually or in synergy with bioactive compounds such as tannins, alkaloids, terpenoids, steroids, tannins, anthraquinones and flavonoids [ 43 , 68 ]. Alkaloids exhibits antiplasmodial activities by blocking protein synthesis in P. falciparum [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

In vivo antiplasmodial activities of stem bark extracts of Avicennia marina in Plasmodium berghei-infected mice

Wilfred¹,

Songoro²,

Gathirwa³

et al. 2023

Pan Afr Med J

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Introduction malaria remains the leading cause of morbidity and mortality in developing tropical and subtropical nations. Due to the emergence and spread of drug resistance to currently available drugs, there is a need for the search of novel, safe, and reasonably affordable anti-malarial medications. The objective of this study was to assess the in vivoanti-malarial effectiveness of Avicennia marina stem bark extracts in a mice model. Methods guidelines 425 of the Organization for Economic Cooperation and Development were used to determine the extracts' acute toxicity. Mice infected with chloroquine-sensitive Plasmodium berghei (ANKA strain) were tested for in vivoanti-plasmodial activity, and by giving oral doses of 100 mg/kg, 250 mg/kg, and 500 mg/kg body weight of extracts, the plant's suppressive, curative, and preventive effects were assessed. Results mice treated with dosages of up to 5000 mg/kg showed no evidence of acute toxicity or mortality. Consequently, it was determined that the acute lethal dosage of Avicennia marina extracts in swiss albino mice was greater than 5000 mg/kg. All doses of the extracts exhibited significant (p<0.05) dose-dependent suppression of P. berghei in the suppressive tests compared to the control group. At the highest dose (500 mg/kg), Methanolic crude extracts exerted the highest (93%) parasitemia suppression during the 4-day suppressive test. The extracts also displayed significant (p<0.001) prophylactic and curative activities at all doses compared to the control. Conclusion results from this study ascertained the safety and promising curative, prophylactic and suppressive anti-plasmodial capabilities of the stem bark extracts of Avicennia marina in mice model.

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Section: Discussionmentioning

confidence: 99%

In vivo antiplasmodial activities of stem bark extracts of Avicennia marina in Plasmodium berghei-infected mice

Wilfred¹,

Songoro²,

Gathirwa³

et al. 2023

Pan Afr Med J

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“…As a result, alkaloids, anthraquinones, phenol, tannins, and flavonoids found in this fraction may have attributed to the discovered antimalarial activity. Alkaloids [ 57 ], flavonoids [ 58 ], phenols [ 59 ], anthraquinones [ 60 ], and tannins [ 61 ] have all been implicated with antimalarial activity. This outcome is concordant with the efficacy of CF reported on CF of Brucea antidysenterica leaves [ 62 ], but higher with the low effect of CF reported on root of Silene macrosolen A.…”

Section: Discussionmentioning

confidence: 99%

Antimalarial Efficacy of Hydromethanolic Root Extract and Solvent Fractions of Urtica simensis Hochst. ex. A. Rich. (Urticaceae): An Experimental Study on Plasmodium berghei-Infected Mice

Sisay

Andargie

Molla

2022

Evidence-Based Complementary and Alternative Medicine

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Background. Despite modern therapeutic armamentariums, malaria remains a 21st century public health menace. The issue of combating malaria is the ever-growing resistance to high-tech medications in which novel phytomedicines are highly demanding, a rapidly expanding research avenue. In Ethiopian folklore medicine, Urtica simensis has been used to treat malaria by drinking its juice after the dry roots have been mashed and combined with water. Hitherto, no in vivo study has been reported in the literature so far. To substantiate this folkloric claim, the present work herein was done. Methods. An acute oral toxicity study was conducted as per the standard protocol. To rule out, the extract’s inherent potential effects on bodyweight, basal body To, and PCV changes were tracked for two weeks. A four-day suppressive model and a curative assay model were utilized to investigate the antimalarial activity of the plant. Percent parasitemia suppression, packed cell volume, mean survival date, bodyweight, and rectal body temperature were used to determine antimalarial activity. Result. An acute toxicity study reveals that Urtica simensis was atoxic at a dose of 2000 mg/kg. It also affirms that U. simensis is free from intrinsic potential effects of interfering with bodyweight, temperature, and packed cell volume evolution. Both crude extract and its solvent fractions at all test doses exerted significant ( P < 0.001 ) inhibition of parasitemia as compared to the control group. CF400 mg/kg provided the greatest chemosuppressive effect (79.24%). In a curative experiment, crude extract and CF were able to prevent the cardinal indications of P. berghei-induced malaria, such as weight loss, hypothermia, parasitemia, and anemia. Both crude extracts and their solvent fractions prolong survival dates. Conclusion. The antimalarial activity of the crude extract and its solvent fractions was promising, confirming previous assertions. As a result, more research studies into chemical entities may be required.

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“…Thirty (30) mice out of 36 were infected each with 200 μL of diluted inoculum containing 1 × 10 7 parasitized erythrocytes intraperitoneally and then randomly divided into 5 groups of six (6) mice each. The remaining six nonparasitized mice formed the normal group as shown below: Mice were inoculated intraperitoneally with parasitized erythrocytes below the diaphragm as described by Azizi et al [14]. Three hours (3 h) after inoculation, the mice were treated for 4 consecutive days (day 0, day 1, day 2, and day 3).…”

Section: Antimalarial Activitymentioning

confidence: 99%

“…At intervals of 24 h, on the 4th to 9th days, thin smears were prepared from the tail blood of each mouse and stained with the May-Grünwald-Giemsa. The parasitemia, average suppression of parasitemia, and mean survival rate were calculated as follows [14]: ). The parasitemia in all experimental groups was evaluated from the 4th to 10th (D3-D9) days.…”

Section: Antimalarial Activitymentioning

confidence: 99%

Efficacy of Khaya grandifoliola Stem Bark Ethanol Extract in the Treatment of Cerebral Malaria in Swiss albino Mice Using Plasmodium berghei NK65 Strain

Guy-Armand,

Cedric,

Nadia

et al. 2023

Journal of Parasitology Research

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Background. Cerebral malaria is one of the most severe and dangerous forms of malaria and is potentially fatal. This study was aimed at evaluating the anticerebral malaria efficacy of Khaya grandifoliola used by traditional healers. Method. Fifty grams of Khaya grandifoliola stem bark was macerated in 1 L ethanol (95%) for 72 h. The filtrate was dried at 40°C until the obtention of a dry extract. The antimalarial test was evaluated using the Peter 4-day suppressive test and the Rane curative test. Mice were group into 6 groups of 6 mice each. For the antioxidant test, parameters such as malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), and nitric oxide (NO) were assessed. The livers of mice were crushed and centrifuged in order to be measured. Aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT) using the Dutch Diagnostics Kit and blood were collected for haematological parameters. Results. The ethanol extract showed a suppressive activity of 78.12%, 75.30%, and 68.69% at 500 mg/kg, 250 mg/kg, and 125 mg/kg, respectively. Similarly, the curative activity showed a statistically significant reduction in parasitemia ( p < 0.05 ). Antioxidant parameter assays showed a low value of MDA and a high value of SOD, CAT, NO, and GSH in the negative control group. A statistically significant higher values of ASAT and ALAT were observed in the negative control compared to the other test groups ( p < 0.05 ). Haematological parameters showed a statistically significant decrease in white blood cells, red blood cells, haemoglobin, and platelets in the negative control group ( p < 0.05 ). Conclusion. The results of this study justify the traditional usage of Khaya grandifoliola in the treatment of cerebral malaria. However, in vivo toxicity assessment is still necessary to verify its safeness.

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In Vivo Antiplasmodial Activity of Entandrophragma cylindricum (Sprague) Sprague Ethyl Acetate Extract in Plasmodium berghei-Infected Mice

Cited by 5 publications

References 16 publications

In vivo antiplasmodial activities of stem bark extracts of Avicennia marina in Plasmodium berghei-infected mice

In vivo antiplasmodial activities of stem bark extracts of Avicennia marina in Plasmodium berghei-infected mice

Antimalarial Efficacy of Hydromethanolic Root Extract and Solvent Fractions of Urtica simensis Hochst. ex. A. Rich. (Urticaceae): An Experimental Study on Plasmodium berghei-Infected Mice

Efficacy of Khaya grandifoliola Stem Bark Ethanol Extract in the Treatment of Cerebral Malaria in Swiss albino Mice Using Plasmodium berghei NK65 Strain

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