In vivo antitumor potential of extracts from different parts of Bauhinia variegata linn. Against b16f10 melanoma tumour model in c57bl/6 mice

Abstract

Background: Melanoma is a metastatic type of skin cancer that is difficult to treat and the majority of efforts are directed to the design of new drugs. Medicinal Plants have been the primary source of medicines since life on earth; more than 50% of existing cancer treatments is derived from plants. Bauhinia variegata is well-known medicinal plant used from the ancient era to till date for their medicinal values. Scientific literatures have not documented any evidence of the antitumour potential of Bauhinia variegata against B16F10 melanoma tumor model in C57BL mice. The present investigation was undertaken to explore the antitumour activity of Leaf, stem bark and flower extract of Bauhinia variegata against B16F10 melanoma tumour model in C57BL mice. Methods: Hydro-methanolic extract prepared from the leaf, stem bark and flower of Bauhinia variegata were assessed for their antitumor activity. The extracts at doses of 500 and 750 mg/kg b.wt. were given orally along with cyclophosphamide (chemotherapeutic drug) for 40 days for exploring antitumor activity against melanoma tumor (B16F10) in C57BL mice. Inhibition of tumor growth, increase in survival time of animal with treatment, histopathological studies and antioxidant parameter were determined. Results: The Present investigation showed significant effect of the B. variegata L. in preventing melanoma tumor by B16F10 cell line in C57BL/6 mice. As compared with the tumour control group, the remarkable results especially in the group which received B. variegata extract and cyclophosphamide together were obtained for all of the measured parameters. Dose dependent response was observed in tumor volume, inhibition rate, life span time and antioxidant parameter of extracts. Combination treatment of cyclophosphamide and B. variegata extracts showed more pronounced effect. Conclusions: These findings suggest that B. variegata hydromethanolic extract may contain bioactive compounds of potential therapeutic significance which are relatively safe from toxic effects, and can compromise the medicinal use of this plant in folk medicine.

Estudo in silico das atividades de compostos isolados de Bauhinia variegata Linn

“…Predições farmacocinéticas das substâncias isoladas de Bauhinia variegata. Pandey, 2017;Mishra, et al, 2013;Rajkapoor, et al, 2003). A análise desse processo é importante tendo em vista que a morte celular programada corresponder a um mecanismo bioquímico que subsidia o controle de células nos organismos multicelulares, promovendo a homeostase dos tecidos e sendo extremamente vital para células infectadas, mutadas ou danificadas (Kruidering & Evan, 2000).…”

Effects of Herbal formulations in management of Uterine Fibroid (Sul’ah-E-Rehm) – A Case Report

“…It was observed that hydromethanolic extracts of leaf, stem bark and flower of Bauhinia variegata,at the dose of 500 and 750 mg / kg of body weight, significantly reduced melanoma tumor volume. [19] This effect may be attributed to the presence of polar phytoconstituents such as alkaloids, flavonoids, tannins, terpenoids, and glycosides present in the crude extract of B. variegata. [19] Camargo et al studied the antitumor potential with extract of Zingiber officinale showed a cytotoxic effect for human kidney tumor cell with no harm effect for the normal cells.…”

“…[19] This effect may be attributed to the presence of polar phytoconstituents such as alkaloids, flavonoids, tannins, terpenoids, and glycosides present in the crude extract of B. variegata. [19] Camargo et al studied the antitumor potential with extract of Zingiber officinale showed a cytotoxic effect for human kidney tumor cell with no harm effect for the normal cells. This effect may be due to the bioactive compounds of Z. Officinale phenolics compounds, total flavonoids, yellow flavonoids and anthocyanins.…”

Botanical from Piper capense Fruit Can Help to Combat the Melanoma as Demonstrated by In Vitro and In Vivo Studies

“…All of these justify the urgency in the search for naturally occurring anticancer drugs with fewer side effects and designed to overcome the resistance problem. Several studies have already been carried out with plant extracts in relation to melanoma cell line B16-F10; for example, Pandey [ 23 ], showed the in vivo antitumor potential of extracts from the different parts of Bauhinia variegata Linn. Rajasekar et al [ 24 ] showed the anticancerous effect of Lithospermum erythrorhizon extract in vitro and in vivo .…”