In vivo assessment of absolute perfusion in the murine skeletal muscle with spin labeling MRI

Streif, Jörg U. G.; Hiller, K. H.; Waller, Christiane; Nahrendorf, Matthias; Wiesmann, Frank; Bauer, Wolfgang R.; Rommel, Eberhard; Haase, Axel

doi:10.1002/jmri.10229

Cited by 35 publications

(26 citation statements)

References 26 publications

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“…Contrast-enhanced MRI has been shown to accurately locate and quantify infarction size in post-MI mouse studies (23). Perfusion studies have been performed in mouse skeletal muscle (19) and in the mouse heart. Furthermore, spatially localized 31 P spectroscopy techniques have been applied to the mouse heart to study cardiac metabolism (1).…”

Section: Discussionmentioning

confidence: 99%

Measurement of myocardial mechanics in mice before and after infarction using multislice displacement-encoded MRI with 3D motion encoding

Gilson¹,

Yang²,

French³

et al. 2005

American Journal of Physiology-Heart and Circulatory Physiology

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Cardiac MRI is an accurate, noninvasive modality for assessing the structure and function of the murine heart. In addition to conventional imaging, MRI tissue tracking methods can quantify numerous aspects of myocardial mechanics, including intramyocardial displacement, strain, twist, and torsion. In the present study, we developed and applied a novel pulse sequence based on displacement-encoded imaging using stimulated echoes (DENSE) that achieves multislice coverage, high spatial resolution, and three-dimensional (3D) displacement encoding. With the use of this technique, myocardial mechanics of C57Bl/6 mice were measured at baseline and 1 day after experimental myocardial infarction. At baseline, the mean systolic transmural circumferential strain was -0.14 +/- 0.02 and the mean systolic radial strain was 0.30 +/- 0.05. Changes in circumferential and radial strains from the subepicardium to the subendocardium were detected at baseline (P < 0.05). One day after infarction, significantly reduced 3D displacements and strain were detected in infarcted and noninfarcted myocardium. Infarction also reduced normalized systolic torsion from its baseline value of 1.35 +/- 0.27 degrees /mm (R = 0.99) to 0.07 +/- 0.54 degrees /mm (R = 0.96, P < 0.05). DENSE MRI can assess the 3D myocardial mechanics of the murine heart in <1 h of scan time at 4.7 T and may be applied to studies of myocardial mechanics in genetically engineered mice.

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Section: Discussionmentioning

confidence: 99%

Measurement of myocardial mechanics in mice before and after infarction using multislice displacement-encoded MRI with 3D motion encoding

Gilson¹,

Yang²,

French³

et al. 2005

American Journal of Physiology-Heart and Circulatory Physiology

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“…Previously, to reduce respiration corruption, researchers have: ventilated animals (8, 14–19, 23), discarded images by visual inspection, assumed constant respiration rate (9–13), or used higher levels of isoflurane and velocity compensating gradients (22, 23, 35). An improvement upon these approaches was presented in Vandsburger et al (21), where acquisitions are double‐gated to reduce respiration corruption and Fuzzy‐C means reclustering is used to compensate for additional mis‐spacing of acquisitions introduced by respiratory gating.…”

Section: Discussionmentioning

confidence: 99%

Cardiac arterial spin labeling using segmented ECG‐gated Look‐Locker FAIR: Variability and repeatability in preclinical studies

Campbell-Washburn

Price

Wells

et al. 2012

Magnetic Resonance in Med

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MRI is important for the assessment of cardiac structure and function in preclinical studies of cardiac disease. Arterial spin labeling techniques can be used to measure perfusion noninvasively. In this study, an electrocardiogram-gated Look-Locker sequence with segmented k-space acquisition has been implemented to acquire single slice arterial spin labeling data sets in 15 min in the mouse heart. A data logger was introduced to improve data quality by: (1) allowing automated rejection of respiration-corrupted images, (2) providing additional prospective gating to improve consistency of acquisition timing, and (3) allowing the recombination of uncorrupted k-space lines from consecutive data sets to reduce respiration corruption. Finally, variability and repeatability of perfusion estimation within-session, between-session, between-animal, and between image rejection criteria were assessed in mice. The criterion used to reject images from the T(1) fit was shown to affect the perfusion estimation. These data showed that the between-animal coefficient of variability (24%) was greater than the between-session variability (17%) and within-session variability (11%). Furthermore, the magnitude of change in perfusion required to detect differences was 30% (within-session) and 55% (between-session) according to Bland-Altman repeatability analysis. These technique developments and repeatability statistics will provide a platform for future preclinical studies applying cardiac arterial spin labeling.

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“…(12). Subsequently, the method was applied to the in vivo rat heart for the determination of basal myocardial perfusion (13) and for the quantification of perfusion in murine skeletal muscle (14). Substantial validation experiments were performed and range from local validation against measurements made with first pass perfusion imaging in the isolated rat heart (15) to comparison of in vivo data from the rat heart with microspheres examinations (16).…”

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confidence: 99%

In vivo assessment of absolute perfusion and intracapillary blood volume in the murine myocardium by spin labeling magnetic resonance imaging

Streif¹,

Nahrendorf²,

Hiller³

et al. 2005

Magnetic Resonance in Med

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The absolute perfusion and the intracapillary or regional blood volume (RBV) in murine myocardium were assessed in vivo by spin labeling magnetic resonance imaging. Pixel-based perfusion and RBV maps were calculated at a pixel resolution of 469 ؋ 469 m and a slice thickness of 2 mm. The T 1 imaging module was a segmented inversion recovery snapshot fast low angle shot sequence with velocity compensation in all three gradient directions. The group average myocardial perfusion at baseline was determined to be 701 ؎ 53 mL (100 g ⅐ min) ؊1 for anesthesia with isoflurane (N ‫؍‬ 11) at a mean heart rate (HR) of 455 ؎ 10 beats per minute (bpm). This value is in good agreement with perfusion values determined by invasive microspheres examinations. For i.v. administration of the anesthetic Propofol, the baseline perfusion decreased to 383 ؎ 40 mL (100 g ⅐ min) ؊1 (N ‫؍‬ 17, P < 0.05 versus. isoflurane) at a mean heart rate of 261 ؎ 13 bpm (P < 0.05 versus isoflurane). In addition, six mice with myocardial infarction were studied under isoflurane anesthesia (HR 397 ؎ 7 bpm). The perfusion maps showed a clear decrease of the perfusion in the infarcted area. The perfusion in the remote myocardium decreased significantly to 476 ؎ 81 mL (100 g ⅐ min) ؊1 (P < 0.05 versus sham). Regarding the regional blood volume, a mean value of 11.8 ؎ 0.8 vol % was determined for healthy murine myocardium under anesthesia with Propofol (N ‫؍‬ 4, HR 233 ؎ 17 bpm). In total, the presented techniques provide noninvasive in vivo assessment of the perfusion and the regional blood volume in the murine myocardium for the first time and seem to be promising tools for the characterization of mouse models in cardiovascular research. Over the past decade, the mouse animal model has become an essential part of medical basic research. This is due to the high degree of similarity between the mouse and the human genomes of about 97% (1) and the resulting high relevance of mouse animal studies for human basic research. Regarding cardiovascular research, magnetic resonance imaging (MRI) provides noninvasive and accurate assessment of cardiac structure and function (2). Since the viability and the capability of the myocardium are strongly influenced by its microcirculation, a full characterization of the myocardium requires a quantification of parameters such as the perfusion and the intracapillary or regional blood volume (RBV). In particular, perfusion is of paramount importance as a physiologic parameter, since it strongly determines the function of organs and the severity of many diseases.Heart diseases involving ventricular dysfunction and hypertrophy show significant alterations of the myocardial perfusion and transgenic mouse models may be well suited to elucidate the underlying fundamental mechanisms.For the assessment of cardiac perfusion in mice, only a few methods have been presented to date. One possible approach is the quantification of the coronary flow in the isolated mouse heart by ultrasonic flow probes (3). The injection of labeled microsphe...

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In vivo assessment of absolute perfusion in the murine skeletal muscle with spin labeling MRI

Cited by 35 publications

References 26 publications

Measurement of myocardial mechanics in mice before and after infarction using multislice displacement-encoded MRI with 3D motion encoding

Measurement of myocardial mechanics in mice before and after infarction using multislice displacement-encoded MRI with 3D motion encoding

Cardiac arterial spin labeling using segmented ECG‐gated Look‐Locker FAIR: Variability and repeatability in preclinical studies

In vivo assessment of absolute perfusion and intracapillary blood volume in the murine myocardium by spin labeling magnetic resonance imaging

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