In vivo assessment of mitochondrial capacity using NIRS in locomotor muscles of young and elderly males with similar physical activity levels

Lagerwaard, Bart; Nieuwenhuizen, Arie G.; Boer, Vincent C.J. de; Keijer, Jaap

doi:10.1007/s11357-019-00145-4

Cited by 32 publications

(42 citation statements)

References 53 publications

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“…These individuals were selected because enough material was available and data on in vivo mitochondrial capacity in the vastus lateralis were previously collected. 21 The transcriptional differences translated into significant lower protein expression of Complex IV and Complex II, or succinate dehydrogenase ( P = 0.011 and P = 0.0009, respectively) ( Figure 4B ), whereas a trend could be observed for Complex I, in older compared with young muscle ( P = 0.062) ( Figure 4C ). The lower protein expression could explain, at least in part, the lower mitochondrial capacity observed in the older compared with the young group, as protein expression of Complexes I and IV was significantly correlated to mitochondrial capacity in the vastus lateralis as measured in vivo using NIRS ( P = 0.017, R 2 = 0.42 and P = 0.030, R 2 = 0.36) ( Figure 4D ).…”

Section: Resultsmentioning

confidence: 96%

Matrisome, innervation and oxidative metabolism affected in older compared with younger males with similar physical activity

Lagerwaard

Nieuwenhuizen

Bunschoten

et al. 2021

J cachexia sarcopenia muscle

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Background Due to the interaction between skeletal muscle ageing and lifestyle factors, it is often challenging to attribute the decline in muscle mass and quality to either changes in lifestyle or to advancing age itself. Because many of the physiological factors affecting muscle mass and quality are modulated by physical activity and physical activity declines with age, the aim of this study is to better understand the effects of early ageing on muscle function by comparing a population of healthy older and young males with similar physical activity patterns. Methods Eighteen older (69 ± 2.0 years) and 20 young (22 ± 2.0 years) males were recruited based on similar self-reported physical activity, which was verified using accelerometry measurements. Gene expression profiles of vastus lateralis biopsies obtained by RNA sequencing were compared, and key results were validated using quantitative polymerase chain reaction and western blot. Results Total physical activity energy expenditure was similar between the young and old group (404 ± 215 vs. 411 ± 189 kcal/day, P = 0.11). Three thousand seven hundred ninety-seven differentially expressed coding genes (DEGs) were identified (adjusted P-value cut-off of <0.05), of which 1891 were higher and 1906 were lower expressed in the older muscle. The matrisome, innervation and inflammation were the main upregulated processes, and oxidative metabolism was the main downregulated process in old compared with young muscle. Lower protein levels of mitochondrial transcription factor A (TFAM, P = 0.030) and mitochondrial respiratory Complexes IV and II (P = 0.011 and P = 0.0009, respectively) were observed, whereas a trend was observed for Complex I (P = 0.062), in older compared with young muscle. Protein expression of Complexes I and IV was significantly correlated to mitochondrial capacity in the vastus lateralis as measured in vivo (P = 0.017, R 2 = 0.42 and P = 0.030, R 2 = 0.36). A trend for higher muscle-specific receptor kinase (MUSK) protein levels in the older group was observed (P = 0.08). Conclusions There are clear differences in the transcriptome signatures of the vastus lateralis muscle of healthy older and young males with similar physical activity levels, including significant differences at the protein level. By disentangling physical activity and ageing, we appoint early skeletal muscle ageing processes that occur despite similar physical activity. Improved understanding of these processes will be key to design targeted anti-ageing therapies.

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Section: Resultsmentioning

confidence: 96%

Matrisome, innervation and oxidative metabolism affected in older compared with younger males with similar physical activity

Lagerwaard

Nieuwenhuizen

Bunschoten

et al. 2021

J cachexia sarcopenia muscle

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“…Future research to confirm these findings could evaluate other domains of muscle quality in the community with advanced technology. These include non-invasive, portable modalities such as quantitative musculoskeletal ultrasound to evaluate muscle structure [ 51 ] and near infra-red spectroscopy (NIRS) to characterize muscle oxidative potential as a proxy for mitochondrial capacity [ 52 ]. Increasing age is also correlated with poorer STS test performance as it may represent progressive diminishing muscle quality over time [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Performance on sit-to-stand tests in relation to measures of functional fitness and sarcopenia diagnosis in community-dwelling older adults

Yee

Allen

et al. 2021

Eur Rev Aging Phys Act

108

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Background The sit-to-stand (STS) test has been deployed as surrogate measures of strength or physical performance in sarcopenia diagnosis. This study examines the relationship of two common STS variants – Five Times Sit-to-Stand Test (5TSTS) and 30 s Chair Stand Test (30CST) – with grip strength, muscle mass and functional measures, and their impact on sarcopenia prevalence in community-dwelling older adults. Methods This is a cross-sectional analysis of 887 community-dwelling adults aged ≥50 years. Participants completed a battery of physical fitness tests - 5TSTS, 30CST, grip strength, gait speed, Timed-Up-and-Go (TUG) for dynamic balance and six-minute walk test (6MWT) for cardiorespiratory endurance. Muscle mass was measured using multi-frequency segmental bioelectrical impedance analysis (BIA). We performed correlation analysis between STS performance and other fitness measures and muscle mass, followed by multiple linear regression for the independent determinants of STS performance. Results Mean participant age was 67.3±7 years, with female predominance (72.9%). STS tests exhibited weak correlations with grip strength (30CST, r = 0.290; 5TSTS, r = − 0.242; both p< 0.01), and stronger correlations with gait speed (30CST, r = 0.517; 5TSTS, r = − 0.533; both p< 0.01), endurance (30CST, r = 0.558; 5TSTS, r = − 0.531; both p < 0.01) and dynamic balance (30CST, r = − 0.501; 5TSTS, r = 0.646; both p< 0.01). Muscle mass correlated with grip strength but not STS. In multiple regression analysis, all fitness measures were independently associated with 30CST performance. Performance in both STS tests remained independent of muscle mass. There was no significant difference in prevalence of possible sarcopenia diagnosis using grip strength or STS (30CST, 25.0%; 5TSTS, 22.1%; grip strength, 22.3%; p = 0.276). When both measures are used, prevalence is significantly higher (42.0%; p = 0.276). Prevalence of confirmed sarcopenia with inclusion of muscle mass was significantly lower using STS compared with grip strength (30CST, 4.6%; 5TSTS, 4.1% vs. grip strength, 7.1%; p< 0.05). Conclusion In the sarcopenia construct, STS tests better represents muscle physical performance rather than muscle strength. Different subsets of population with possible sarcopenia are identified depending on the test used. The lack of association of STS performance with muscle mass results in a lower prevalence of confirmed sarcopenia compared with grip strength, but may better reflect changes in muscle quality.

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“…36 However, as muscle function in postmenopausal females declines more rapidly compared to males, 37 the ageing trajectory for males and females may be different, [38][39][40] in particular with respect to skeletal muscle tissue. 36,41,42 Although the mitochondrial function in the vastus lateralis was shown to decrease with aging in males, [43][44][45] it may be that males, displaying a larger muscle mass and higher physical activity levels, 46,47 are more resilient to aging and less prone to frailty compared to females, which is suggested by the significantly reduced scores for the 400 m walk test and the SPPB in the (pre-)frail females but not in the males.…”

Section: Discussionmentioning

confidence: 99%

Intramuscular short‐chain acylcarnitines in elderly people are decreased in (pre‐)frail females, but not in males

et al. 2020

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This study tested the hypothesis that in human aging, a decreased intramuscular acylcarnitine status is associated with (pre‐)frailty, reduced physical performance, and altered mitochondrial function. We used a cross‐sectional study design with well‐matched fit and (pre‐)frail old males and females, using young males and females as healthy controls. Frailty was assessed according to the Fried criteria and physical performance was determined by 400 m walk test, short physical performance battery and handgrip strength. Muscle and plasma acylcarnitine status, and muscle mitochondrial gene expression was analyzed. Results showed that intramuscular total carnitine levels and short‐chain acylcarnitine levels were lower in (pre‐)frail old females compared to fit old females and young females, whereas no differences were observed in males. The low intramuscular short‐chain acylcarnitine levels in females correlated with low physical performance, even after correction for muscle mass (%), and were accompanied with lowered expression of genes involved in mitochondrial energy production and functionality. It is, therefore, concluded that in (pre‐)frail old females, intramuscular total carnitine levels and short‐chain acylcarnitine levels are decreased, and this decrease is associated with reduced physical performance and low expression of a wide range of genes critical for mitochondrial function. The results stress the importance of taking sex differences into account in aging research.

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In vivo assessment of mitochondrial capacity using NIRS in locomotor muscles of young and elderly males with similar physical activity levels

Cited by 32 publications

References 53 publications

Matrisome, innervation and oxidative metabolism affected in older compared with younger males with similar physical activity

Matrisome, innervation and oxidative metabolism affected in older compared with younger males with similar physical activity

Performance on sit-to-stand tests in relation to measures of functional fitness and sarcopenia diagnosis in community-dwelling older adults

Intramuscular short‐chain acylcarnitines in elderly people are decreased in (pre‐)frail females, but not in males

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