In Vivo Assessment of Neovascularization and Incorporation of Prosthetic Vascular Biografts

Menger, Michael D.; Hammersen, F.; Meßmer, K.

doi:10.1055/s-2007-1020105

Cited by 42 publications

(31 citation statements)

References 10 publications

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“…The dorsal skinfold chamber is a widely used and well-accepted model in microcirculatory research in hamsters and mice. [12][13][14][15][16][17][18] Scaffold manufacturing and characteristics A block-copolymer polymer composed of alternating soft blocks of polyethylene glycol terephthalate (PEGT) and hard blocks of polybutylene terephthalate (PBT) was used. Polymer composition can be modified by varying the weight percentages of PEGT and PBT or by changing the molecular weight (MW) of the PEG.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Neovascularization of poly(ether ester) block‐copolymer scaffolds in vivo: Long‐term investigations using intravital fluorescent microscopy

Druecke

Langer

Lamme³

et al. 2003

J Biomedical Materials Res

173

138

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Poly(ether ester) block-copolymer scaffolds of different pore size were implanted into the dorsal skinfold chamber of balb/c mice. Using intravital fluorescent microscopy, the temporal course of neovascularization into these scaffolds was quantitatively analyzed. Three scaffold groups (diameter, 5 mm; 220 -260 thickness, m; n ϭ 30) were implanted. Different pore sizes were evaluated: small (20 -75 m), medium (75-212 m) and large pores (250 -300 m). Measurements were performed on days 8, 12, 16, and 20 in the surrounding normal tissue, in the border zone, and in the center of the scaffold. Standard microcirculatory parameters were assessed (plasma leakage, vessel diameter, red blood cell velocity, and functional vessel density). The largepored scaffolds showed significantly higher functional vessel density in the border zone and in the center (days 8 and 12) compared with the scaffold with the small and mediumsized pores. These data correlated with a larger vessel diameter and a higher red blood cell velocity in the largepored scaffold group. Interestingly, during the evaluation period the microcirculatory parameters on the edge of the scaffolds returned to values similar to those found in the surrounding tissue. In the center of the scaffold, however, neovascularization was still active 20 days after implantation. Plasma leakage and vessel diameter were higher in the center of the scaffold. Red blood cell velocity and functional vessel density were 50% lower than in the surrounding tissue. In conclusion, the dorsal skinfold chamber model in mice allows long-term study of blood vessel growth and remodeling in porous biomedical materials. The rate of vessel ingrowth into poly(ether ester) block-copolymer scaffolds is influenced by pore size and was highest in the scaffold with the largest pores. The data generated with this model contribute to knowledge about the development of functional vessels and tissue ingrowth into biomaterials.

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Section: Surgical Proceduresmentioning

confidence: 99%

Neovascularization of poly(ether ester) block‐copolymer scaffolds in vivo: Long‐term investigations using intravital fluorescent microscopy

Druecke

Langer

Lamme³

et al. 2003

J Biomedical Materials Res

173

138

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Section: Mw Laschke Et Al Biomaterials Research With the Skinfold Chmentioning

confidence: 99%

“…Biosynthetic composite grafts consisting of ovine collagen and polyester (Omnifl ow ® ) have shown an early vascularisation and tight incorporation into the surrounding chamber tissue (Menger et al, 1992c). Of interest, this has not been the case for biolised bovine artery grafts (SolcoP ® ), which may be due to the formation of a fi brous collagen capsule around the implants (Menger et al, 1992c). In a recent study, Esguerra et al (2010) used the dorsal skinfold chamber model to analyse the in vivo vascularisation and biocompatibility of bacterial cellulose as a potential novel biomaterial for the reconstruction of small-diameter blood vessels.…”

Section: Mw Laschke Et Al Biomaterials Research With the Skinfold Chmentioning

confidence: 99%

“…In addition to microscopy, several other non-invasive techniques have been used in the past for the analysis of microcirculatory parameters within the dorsal skinfold chamber, including Laser Doppler flowmetry for the assessment of microvascular tissue perfusion (Menger et al, 1992b) or phosphorescence quenching for the measurement of tissue oxygenation (Kerger et al, 1996). However, these techniques are indirect in nature and, thus, do not allow for the direct visualisation of distinct cellular …”

Section: Mw Laschke Et Almentioning

confidence: 99%

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The dorsal skinfold chamber: window into the dynamic interaction of biomaterials with their surrounding host tissue

Laschke

Vollmar²,

Menger³

2011

eCM

146

128

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The implantation of biomaterials into the human body has become an indispensable part of almost all fi elds of modern medicine. Accordingly, there is an increasing need for appropriate approaches, which can be used to evaluate the suitability of different biomaterials for distinct clinical indications. The dorsal skinfold chamber is a sophisticated experimental model, which has been proven to be extremely valuable for the systematic in vivo analysis of the dynamic interaction of small biomaterial implants with the surrounding host tissue in rats, hamsters and mice. By means of intravital fl uorescence microscopy, this chronic model allows for repeated analyses of various cellular, molecular and microvascular mechanisms, which are involved in the early infl ammatory and angiogenic host tissue response to biomaterials during the initial 2-3 weeks after implantation. Therefore, the dorsal skinfold chamber has been broadly used during the last two decades to assess the in vivo performance of prosthetic vascular grafts, metallic implants, surgical meshes, bone substitutes, scaffolds for tissue engineering, as well as for locally or systemically applied drug delivery systems. These studies have contributed to identify basic material properties determining the biocompatibility of the implants and vascular ingrowth into their surface or internal structures. Thus, the dorsal skinfold chamber model does not only provide deep insights into the complex interactions of biomaterials with the surrounding soft tissues of the host but also represents an important tool for the future development of novel biomaterials aiming at an optimisation of their biofunctionality in clinical practice.

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“…37 Furthermore, the chamber model is ideal for transplantation and implantation experiments, because the cover glass of the observation window can temporarily be removed. The skinfold chamber model has been used to study angiogenesis within various physiological tissues [38][39][40][41][42] and malignant tumors, 43,44 as well as biomaterials such as synthetic and biosynthetic vascular grafts [45][46][47] and metallic implants. [48][49][50] Using this model, the quantification of the angiogenic process is possible using the combination of epi-illumination multi-fluorescence microscopy and computer-assisted off-line analysis techniques (Fig.…”

Section: The Dorsal Skinfold Chambermentioning

confidence: 99%

Angiogenesis in Tissue Engineering: Breathing Life into Constructed Tissue Substitutes

et al. 2006

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Long-term function of three-dimensional (3D) tissue constructs depends on adequate vascularization after implantation. Accordingly, research in tissue engineering has focused on the analysis of angiogenesis. For this purpose, 2 sophisticated in vivo models (the chorioallantoic membrane and the dorsal skinfold chamber) have recently been introduced in tissue engineering research, allowing a more detailed analysis of angiogenic dysfunction and engraftment failure. To achieve vascularization of tissue constructs, several approaches are currently under investigation. These include the modification of biomaterial properties of scaffolds and the stimulation of blood vessel development and maturation by different growth factors using slow-release devices through pre-encapsulated microspheres. Moreover, new microvascular networks in tissue substitutes can be engineered by using endothelial cells and stem cells or by creating arteriovenous shunt loops. Nonetheless, the currently used techniques are not sufficient to induce the rapid vascularization necessary for an adequate cellular oxygen supply. Thus, future directions of research should focus on the creation of microvascular networks within 3D tissue constructs in vitro before implantation or by co-stimulation of angiogenesis and parenchymal cell proliferation to engineer the vascularized tissue substitute in situ.

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In Vivo Assessment of Neovascularization and Incorporation of Prosthetic Vascular Biografts

Cited by 42 publications

References 10 publications

Neovascularization of poly(ether ester) block‐copolymer scaffolds in vivo: Long‐term investigations using intravital fluorescent microscopy

Neovascularization of poly(ether ester) block‐copolymer scaffolds in vivo: Long‐term investigations using intravital fluorescent microscopy

The dorsal skinfold chamber: window into the dynamic interaction of biomaterials with their surrounding host tissue

Angiogenesis in Tissue Engineering: Breathing Life into Constructed Tissue Substitutes

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