In Vivo Assessment of Thickness and Reflectivity in a Rat Outer Retinal Degeneration Model with Ultrahigh Resolution Optical Coherence Tomography

Hariri, Sanaz; Moayed, Alireza Akhlagh; Choh, Vivian; Bizheva, Kostadinka

doi:10.1167/iovs.11-8395

Cited by 37 publications

(27 citation statements)

References 39 publications

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“…[20][21][22] The high resolution of third-generation spectral-domain OCT devices renders in vivo retinal imaging in mice and rats possible, gaining an increasing importance in ophthalmological and neurological preclinical research. [23][24][25][26][27][28][29][30][31][32][33] The obtained results are in good accordance with histological sections of the animals' retinae. 34 The application of OCT technology in rodent models, however, is still challenging, mainly because of the small size of the rodents' eyes.…”

Section: Introductionsupporting

confidence: 82%

Whole-body positional manipulators for ocular imaging of anaesthetised mice and rats: a do-it-yourself guide

et al. 2016

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BackgroundIn vivo retinal imaging of rodents has gained a growing interest in ophthalmology and neurology. The bedding of the animals with the possibility to perform adjustments in order to obtain an ideal camera-to-eye angle is challenging.MethodsWe provide a guide for a cost-effective, do-it-yourself rodent holder for ocular imaging techniques. The set-up was tested and refined in over 2000 optical coherence tomography measurements of mice and rats.ResultsThe recommended material is very affordable, readily available and easily assembled. The holder can be adapted to both mice and rats. A custom-made mouthpiece is provided for the use of inhalant anaesthesia. The holder is highly functional and assures that the rodent’s eye is the centre of rotation for adjustments in both the axial and the transverse planes with a major time benefit over unrestrained positioning of the rodents.ConclusionWe believe this guide is very useful for eye researchers focusing on in vivo retinal imaging in rodents as it significantly reduces examination times for ocular imaging.

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Section: Introductionsupporting

confidence: 82%

Whole-body positional manipulators for ocular imaging of anaesthetised mice and rats: a do-it-yourself guide

et al. 2016

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“…29 Sodium iodate (NaIO 3 ) has been the most widespread retinotoxin used up to now to induce retinal degeneration resembling GA in animal models. 30 Systemic administration of NaIO 3 in mice, with doses ranging from 20 to 40 mg/mL, is known to selectively impair the RPE, resulting in patchy loss of RPE and subsequent degeneration of photoreceptors. 31 When it was used intravenously in a mouse model, the extent of RPE damage was dependent on the concentration of NaIO 3 and the time elapsed after injection.…”

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confidence: 99%

A Swine Model of Selective Geographic Atrophy of Outer Retinal Layers Mimicking Atrophic AMD: A Phase I Escalating Dose of Subretinal Sodium Iodate

Monés

Leiva

Peña

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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The swine model of a controlled area of circumscribed retinal damage, with well-delimited borders, and selectively of the outer layers of the retina presented herein shows several clinical and histologic features of geographic atrophy in AMD. Therefore, it may represent a valuable tool in the investigation of new emerging regenerative therapies that aim to restore visual function, such as stem cell transplantation or optogenetics.

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“…A similar sequence of events as visualized by OCT was also described in previous studies using IV sodium iodate at doses above 30 mg/kg in both mice and rat models. 16,28,34 Fundus autofluorescence images display increasing irregularity over time, with formation of patchy hyper-and hypofluorescent spots. This may represent areas of photoreceptor debris accumulation and atrophy, respectively.…”

Section: Course Of Sodiummentioning

confidence: 99%

Course of Sodium Iodate–Induced Retinal Degeneration in Albino and Pigmented Mice

Chowers

Cohen

Marks-Ohana

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. To characterize the course of sodium iodate (SI)-induced retinal degeneration in young adult albino and pigmented mice. (25, 50, and 100 mg/kg) were performed in 7-to 8-week-old BALB/c and C57Bl/6J mice. Retinal function and structure was assessed at baseline, 24 hours, 3 days, 1, 2, 3, and 4 weeks postinjection by optokinetic tracking response, ERG, optical coherence tomography (OCT), and histologic and immunohistochemical techniques. METHODS. Single intraperitoneal (IP) injections of SI RESULTS.The 50 mg/kg SI dosage was selected after dose ranging due to consistent retinal effects and lack of systemic toxicity. Time-dependent deterioration in retinal function and morphology was consistently observed between 1 and 4 weeks in all measured parameters. These include reduction of ERG responses, thinning of retinal layers as observed by OCT and histology, and loss of RPE nuclei. Immunohistochemistry revealed rapid RPE disorganization with loss of tight junctions and markedly reduced expression of RPE65 and rod opsin, accompanied by mislocalization of cone opsins. Earlier time points displayed variable results, including partial recovery of visual acuity at 1 week and supranormal ERG cone responses at 24 hours, suggesting possible limitations of early intervention and assessment in the SI model. CONCLUSIONS.A single IP injection of 50 mg/kg SI leads to severe RPE injury followed by vision impairment, dysfunction, and loss of photoreceptors in both BALB/c and C57Bl/6J mice. This easily induced and reproducible noninherited model may serve as a useful tool for seeking and evaluating novel therapeutic modalities for the treatment of retinal degenerations caused by primary failure of the RPE.Keywords: sodium iodate, RPE toxicity, retinal degeneration, mouse model D iseases such as AMD, Best disease, and subtypes of retinitis pigmentosa RP are major causes of visual disability. 1 In these diseases, primary dysfunction, degeneration, and loss of the RPE ultimately results in secondary death of photoreceptors, leading to visual loss. [2][3][4] Despite certain progress in the treatment of retinal degeneration, it remains an essentially irreversible process in which many patients eventually lose their sight. Animal models provide an invaluable tool for searching and testing of novel therapeutic modalities. Animals carrying natural mutations (usually in one gene) provide well-established models for inherited retinal diseases. 5,6 However, the pathogenesis of multifactorial retinal degenerations such as AMD involves complex genetic and environmental factors, leading to expression of disease at older ages. 7 The majority of geneticallyinherited animal models of ocular disease do not entirely mimic such conditions because of early disease onset and progression. 8 Regardless of etiology, maintaining proper RPE function is a major therapeutic target in these diseases, and currently pharmaceutical, genetic, and cellular approaches are being tested to this end in a variety of in vitro and in vivo models, as well as in clini...

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In Vivo Assessment of Thickness and Reflectivity in a Rat Outer Retinal Degeneration Model with Ultrahigh Resolution Optical Coherence Tomography

Abstract: UHR-OCT permits in vivo, noninvasive, longitudinal, quantitative assessment of the progressive changes in retinal morphology and optical reflectivity in a sodium iodate rodent model of outer retinal degeneration.

Cited by 37 publications

References 39 publications

Whole-body positional manipulators for ocular imaging of anaesthetised mice and rats: a do-it-yourself guide

Whole-body positional manipulators for ocular imaging of anaesthetised mice and rats: a do-it-yourself guide

A Swine Model of Selective Geographic Atrophy of Outer Retinal Layers Mimicking Atrophic AMD: A Phase I Escalating Dose of Subretinal Sodium Iodate

Course of Sodium Iodate–Induced Retinal Degeneration in Albino and Pigmented Mice

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