In Vivo Behavior of Decellularized Vein Allograft1,2

Martin, Niels D.; Schaner, Patrick J.; Tulenko, Thomas N.; Shapiro, Irving M.; DiMatteo, Christopher; Williams, Timothy K.; Hager, Eric; DiMuzio, Paul

doi:10.1016/j.jss.2005.06.037

Cited by 64 publications

(52 citation statements)

References 17 publications

(20 reference statements)

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“…Roh, et al [21] demonstrated that bone marrow mononuclear cells, seeded on tissue-engineered grafts, cause migration of monocytes from the surrounding tissues and their self-differentiation. This phenomena was not observed after carotid artery substitution with decellularized veins [22]. We assume that venous wall leukocyte infiltration, which was observed in our study, could be a part of the inflammation-mediated vascular remodeling and is dependent on blood flow.…”

Section: Immunohistologysupporting

confidence: 51%

Transplantation of Decellularized Venous Valvular Grafts in an Ovine Model

Mogaldea¹

2017

Int J Transplant Res Med

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The surgical treatment of end-stage chronic venous insufficiency involves valvular repair or transplantation to restore venous valve function and structure. Current valve substitutes can have issues with durability, thrombogenicity, susceptibility to infection, and a lack of growth potential. Therefore, the development of tissue-engineered venous valves represents a promising solution. Before clinical use of these grafts, in vivo animal testing is necessary. Here we describe the development of different surgical techniques for tissue-engineered venous valve transplantation in a low-pressure system (venous circulation) in a large animal model. Tissue-engineered vein valves prostheses were generated using decellularized jugular ovine Vein Valves (oVV) reseeded with ovine peripheral Blood-derived Endothelial Cells (oBEC). oVV were transplanted orthotopically and heterotopically in an ovine model. Four oVV were transplanted into the jugular position (two decellularized and two decellularized/reseeded vein valves). Two decellularized oVV were heterotopically transplanted into the superior vena cava after induction of Tricuspid Valve Insufficiency (TVI). All animals survived the surgery. After three months, grafts were explanted for histological evaluation. All grafts were permeable and the vascular wall was thickened compared with the native vein. Macroscopic examination revealed loss of valve cusps in all cases. Despite a postoperative regimen of anticoagulants, intraluminal thrombi of various dimensions were found in two implants. In all cases, the histology showed a distinct cell infiltration of the vein wall. Intrathoracic implantation and increase of venous pressure in systole did not prevent venous valve alteration. These findings suggest that proposed approaches are not a suitable for in vivo testing of such grafts.

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Section: Immunohistologysupporting

confidence: 51%

Transplantation of Decellularized Venous Valvular Grafts in an Ovine Model

Mogaldea¹

2017

Int J Transplant Res Med

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“…14,15 Similarly, endothelial progenitor cells are also available in limited quantities and are difficult to isolate and culture. 16 Our group [17][18][19][20][21] and others [22][23][24][25] have shown that adipose tissue is a source of readily available, autologous stem cells with the potential for use as EC substitutes. We have demonstrated that EC growth supplement (ECGS) and shear stress stimulate ASCs to acquire several EC characteristics, such as expression of CD31 (platelet-EC adhesion molecule), uptake of acetylated low-density lipoproteins, alignment in the direction of fluid flow, and formation of capillary-like structures when plated on Matrigel.…”

Section: Introductionmentioning

confidence: 99%

Linear Shear Conditioning Improves Vascular Graft Retention of Adipose-Derived Stem Cells by Upregulation of the α₅β₁ Integrin

McIlhenny

Hager

Grabo

et al. 2010

Tissue Engineering Part A

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Use of adult adipose-derived stem cells (ASCs) as endothelial cell substitutes in vascular tissue engineering is attractive because of their availability. However, when seeded onto decellularized vascular scaffolding and exposed to physiological fluid shear force, ASCs are physically separated from the graft lumen. Herein we have investigated methods of increasing initial ASC attachment using luminal precoats and a novel protocol for the gradual introduction of shear stress to optimize ASC retention. Fibronectin coating of the graft lumen increased ASC attachment by nearly sixfold compared with negative controls. Gradual introduction of near physiological fluid shear stress using a novel bioreactor whereby flow rate was increased every second at a rate of 1.5 dynes/cm(2) per day resulted in complete luminal coverage compared with near complete cell loss following conventional daily abrupt increases. An upregulation of the alpha(5)beta(1) integrin was evinced following exposure to shear stress, which accounts for the observed increase in ASC retention on the graft lumen. These results indicated a novel method for seeding, conditioning, and retaining of adult stem cells on a decellularized vein scaffold within a high-shear stress microenvironment.

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“…[28][29][30] For example, decellularized biomaterials can be seeded with various types of cells to generate functional tissues, [1][2][3]11,31,32 and have the potential for repair, growth, and remodeling in vivo. 16,[32][33][34] The standard of a good decellularization methodology is a combination of complete removal of cellular and nuclear materials for decreased immunogenicity, while minimizing tissue disruption to retain native extracellular matrix structure, with maximal maintenance of mechanical properties for in vivo functionality. A large number of decellularization methods have been reported, which include the utilization of a variety of physical, chemical, and enzymatic approaches to allow the initial lysis of cell and nuclear membranes, the subsequent solubilization of cytoplasmic and nuclear material, and the final removal of all cellular remnants.…”

mentioning

confidence: 99%