In vivo behaviour of a temperature-sensitive (ts) mutant of herpesvirus hominis type 2

Zygraich, N.; Huygelen, C.

doi:10.1007/bf01249353

Cited by 20 publications

(9 citation statements)

References 6 publications

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“…However, from the work of Klemperer et al (1967) and Thouless & Wildy (1975) at least it can be said that in the case of our mutants the only serologically recognized function missing is that of the TK gene. Aside from this it is well known that some temperature sensitive mutants of herpes simplex virus also have low virulence for mice (Zygraich & Huygelen, 1973;Lofgren et al 1977 and our own unpublished observations).…”

Section: Discussionmentioning

confidence: 99%

The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice

Field¹,

Wildy²

1978

J. Hyg.

327

148

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SUMMARYThe pathogenicity for mice of two mutants of herpes simplex virus (type 1 and type 2), which fail to induce thymidine kinase, were compared with their respective parent strains. The mutants were much less virulent than the parents following either intracerebral or peripheral inoculation. The replication of the virus at the site ofinoculation and its progression into the nervous system were studied. Following a very large inoculum in the ear, the type 1 mutant was found to establish a latent infection in the cervical dorsal root ganglia. Mice inoculated intracerebrally with small doses of the mutant viruses were solidly immune to challenge with lethal doses of the parent strain.

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Section: Discussionmentioning

confidence: 99%

The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice

Field¹,

Wildy²

1978

J. Hyg.

327

148

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“…Isolates of small-plaque variants of HSV-I [2] and other variants or mutated viruses generally tend to be less virulent than their large-plaque counterparts [I]. Z y g r a ich and Huygelen [8] have reported similar reduced neurovirulence with their ts mutants of HSV-2. Although this observation has been repeatedly made, no satisfactory explanation has yet been put forward for the phenomenon observed.…”

Section: Discussionmentioning

confidence: 87%

<i>In vivo </i>Characteristics of Temperature-Sensitive Host Range Mutants of Herpes Simplex Virus Type 2

Koment¹,

Rapp²

1975

Intervirology

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The in vivo properties of four host range temperature-sensitive (ts) mutants of herpes simplex virus type 2 (HSV-2) were correlated with known in vitro characteristics. Mutants and parental virus were inoculated into newborn and weanling mice and weanling hamsters by intracranial and subcutaneous routes, and into weanling rabbits by corneal scarification. In all cases the pattern of attenuation of mutants in vivo correlated with their stability in vitro at 39°. The most attenuated mutant (mutant 69) was also the most consistent in its inability to induce cytopathic effects (CPE) in vitro or to replicate under nonpermissive conditions. Conversely, the most virulent mutant (mutant 41) was the least stable under nonpermissive conditions.

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“…In our experiments, no difference was found in the size or shape of plaques caused by HV cl-1 and HV cl-2, nor in their growth in Vero E6 cells and PECs of mice. Since temperature sensitivity determines the virulence of some viruses (Ghendon et al, 1973;McCahon et al, 1981;Mackenzie, 1975;Mills et al, 1971;Murphy et al, 1972;Preble et al, 1975 ;Zygraich et al, 1973), plaque assays were carried out at high and normal temperatures. However no difference was found in viral growth of two variants at these different temperatures.…”

Section: Discussionmentioning

confidence: 99%

Pathogenesis of Hantaan Virus in Mice

Tamura

Asada

Kondo

et al. 1989

Journal of General Virology

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SUMMARYThe virulence of two virus clones (HV cl-I and HV cl-2) of Hantaan virus, which were plaque-purified on Vero E6 cells, were compared in suckling mice infected by the intraperitoneal or intracerebral route. HV cl-1 increased the mortality of the mice whereas HV cl-2 did not. Furthermore, virus of high titre was isolated from various organs of mice infected with HV cl-1 and high titres were maintained, whereas after infection with HV cl-2, virus was isolated from various organs only in low titre and only temporarily. HV cl-1 strongly induced cell-to-cell fusion, but the cell fusion activity was at a minimum level in cells infected with HV cl-2. However these two clones induced similar titres of antibodies in mice. Cytotoxic T lymphocyte assays against macrophages infected with homologous and heterologous virus showed that cytotoxic T cell activity was induced in mice infected with HV cl-2, but suppressed in mice infected with HV cl-1. These results suggest that an alteration in the cell fusion function and the cytotoxic T cell activity are important in the pathogenesis of Hantaan virus infection in newborn mice.

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In vivo behaviour of a temperature-sensitive (ts) mutant of herpesvirus hominis type 2

Cited by 20 publications

References 6 publications

The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice

The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice

<i>In vivo </i>Characteristics of Temperature-Sensitive Host Range Mutants of Herpes Simplex Virus Type 2

Pathogenesis of Hantaan Virus in Mice

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