In Vivo Bioactivities of Hoya parasitica (Wall.) and In Silico Study against Cyclooxygenase Enzymes

Abstract

Hoya parasitica (Wall.) is extensively used in traditional medicine for the treatment of various diseases including rheumatism, kidney problems, jaundice, urinary tract disorders, fever, and pain. The present study was designed to explore new lead compound(s) to alleviate pain, pyresis, and diarrhea from methanol, ethyl acetate, and n-hexane extracts of H. parasitica (Wall.) leaves (MHP, EAHP, and NHP, respectively). Analgesic activity of the extracts was assessed through acetic acid induced writhing, tail immersion, and hot plate tests while brewer’s yeast-induced pyrexia test was employed for the assessment of antipyretic activity. Besides, castor oil and magnesium sulfate induced diarrheal tests were utilized for the evaluation of antidiarrheal properties. Moreover, in silico study of the isolated compounds was undertaken to seek out best-fit phytoconstituent(s) against cyclooxygenase enzymes. MHP revealed substantial antioxidant activities in different in vitro assays compared to EAHP and NHP. In the acetic acid-induced writhing test, among the extracts, MHP (400 mg/kg) revealed maximum 74.15 ± 1% inhibition of writhing comparable to that of standard (85.77 ± 1.39%). Again, in tail immersion and hot plate tests, higher doses of all the test samples exhibited a significant increase of latent period in a time-dependent manner. In brewer yeast-induced pyrexia test, at 3rd and 4th hour of treatment, significant ( P < 0.05 ) antipyretic action was found in the test samples. In both castor oil and magnesium induced diarrheal tests, MHP at 400 mg/kg showed the highest percent inhibition of diarrhea (68.62 ± 4.74 and 64.99 ± 2.90, respectively). Moreover, molecular docking analysis corroborated the results of the present study. The findings of the present study supported the traditional uses of this plant for the alleviation of pain and fever. Furthermore, hoyasterone was found to be the most effective lead compound as cyclooxygenase enzyme inhibitor.

Exploring antioxidative, cytotoxic and neuropharmacological insights into Bixa orellana leaves: Experimental and in silico approaches

“…Folin-Ciocalteu (FC) reagent was employed to assess TPC of plant extracts reported by Sarkar et al, 2022 [ 23 ] and Medha et al, 2022 [ 24 ], where gallic acid standard curve obtained from different concentrations in the range of 100–500 μg/mL, were used to estimate TPC and the findings were interpreted as milligram gallic acid equivalent per gram of dry extract (mg GAE/g).…”

“…TFC of NBOL and MBOL was measured using a standard quercetin calibration curve which was created through several concentrations of quercetin including 0.1, 0.2, 0.3, 0.4 and 0.5 mg/mL [ 23 ]. The absorbance at 510 nm was measured in comparison to a blank, and the TFC of plant extracts was expressed as milligrams of quercetin equivalent per gram of dry extract (mg QE/g).…”

Design, Synthesis, Characterization, Enzyme Inhibition, Molecular Docking, and Pharmacological Evaluation of New Chalcone‐Sulfonate Derivatives Bearing Thiophene

“…Studies with molecular docking applications to reveal the interactions between proteins and ligands attract the great attention of researchers. [47,48] In our previous study, the Root Mean Square Deviation (RMSD) of standards for PL (tetraethylene glycol monooctyl ether) was found and utilized for validation in this study. [34] The interaction affinity values of chalcone-sulfonate derivatives bearing thiophene motif (5 a-j) with PL (1ETH) were depicted in Table 5.…”

In Silico and in Vitro Biological Evaluation of Novel Serial Sulfonate Derivatives on Pancreatic Lipase Activity

“…Thanks to molecular docking applications being frequently preferred in terms of revealing interactions between proteins and ligands, molecular docking studies are of great interest to researchers. [25][26][27] Root Mean Square Deviation (RMSD) of standards with pancreatic lipase (tetraethylene glycol monooctyl ether) value was calculated in our previous study and used for validation in this study. [28] Table 1.…”