In vivo biocompatibility and osteogenesis of electrospun poly(ε-caprolactone)–poly(ethylene glycol)–poly(ε-caprolactone)/nano-hydroxyapatite composite scaffold

Fu, Shaozhi; Ni, PeiYan; Wang, BeiYu; Chu, Bingyang; Peng, Jinrong; Zheng, Lan; Zhao, Xia; Luo, Feng; Wei, Yuquan; Zhang, Qian

doi:10.1016/j.biomaterials.2012.08.023

Cited by 106 publications

(56 citation statements)

References 44 publications

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“…In recent years, electrospun CaP/polymer nanofibrous composites have been recognized as beneficial for the attachment, proliferation, and osteogenic differentiation of osteoblasts [14–16], as well as improving the efficiency of bone defect repair [10, 17, 18]. However, the mechanism behind the supportive function of these scaffolds is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs andIn VivoBone Formation by Activating Ca²⁺-Sensing Receptor Signaling

Zhang

Meng

Huang

et al. 2015

Stem Cells International

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Calcium phosphate- (CaP-) based composite scaffolds have been used extensively for the bone regeneration in bone tissue engineering. Previously, we developed a biomimetic composite nanofibrous membrane of gelatin/β-tricalcium phosphate (TCP) and confirmed their biological activity in vitro and bone regeneration in vivo. However, how these composite nanofibers promote the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is unknown. Here, gelatin/β-TCP composite nanofibers were fabricated by incorporating 20 wt% β-TCP nanoparticles into electrospun gelatin nanofibers. Electron microscopy showed that the composite β-TCP nanofibers had a nonwoven structure with a porous network and a rough surface. Spectral analyses confirmed the presence and chemical stability of the β-TCP and gelatin components. Compared with pure gelatin nanofibers, gelatin/β-TCP composite nanofibers caused increased cell attachment, proliferation, alkaline phosphatase activity, and osteogenic gene expression in rat BMSCs. Interestingly, the expression level of the calcium-sensing receptor (CaSR) was significantly higher on the composite nanofibrous scaffolds than on pure gelatin. For rat calvarial critical sized defects, more extensive osteogenesis and neovascularization occurred in the composite scaffolds group compared with the gelatin group. Thus, gelatin/β-TCP composite scaffolds promote osteogenic differentiation of BMSCs in vitro and bone regeneration in vivo by activating Ca2+-sensing receptor signaling.

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Section: Introductionmentioning

confidence: 99%

Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs andIn VivoBone Formation by Activating Ca²⁺-Sensing Receptor Signaling

Zhang

Meng

Huang

et al. 2015

Stem Cells International

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“…Hydroxyapatite (HAP) is a bioceramic material that has good biocompatibility and bioactivity, and can form a strong bonding with bone tissue678. While the application of HAP alone for bone scaffold is restricted due to its brittleness and difficult processing910.…”

mentioning

confidence: 99%

Graphene oxide as an interface phase between polyetheretherketone and hydroxyapatite for tissue engineering scaffolds

Peng

Feng

et al. 2017

Sci Rep

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The poor bonding strength between biopolymer and bioceramic has remained an unsolved issue. In this study, graphene oxide (GO) was introduced as an interface phase to improve the interfacial bonding between polyetheretherketone (PEEK) and hydroxyapatite (HAP) for tissue engineering scaffolds. On the one hand, the conjugated structure of GO could form strong π-π stacking interaction with the benzene rings in PEEK. On the other hand, GO with a negatively charge resulting from oxygen functional groups could adsorb the positively charged calcium atoms (C sites) of HAP. Consequently, the dispersibility and compatibility of HAP in the PEEK matrix increased with increasing GO content up to 1 wt%. At this time, the compressive strength and modulus of scaffolds increased by 79.45% and 42.07%, respectively. Furthermore, the PEEK-HAP with GO (PEEK-HAP/GO) scaffolds possessed the ability to induce formation of bone-like apatite. And they could support cellular adhesion, proliferation as well as osteogenic differentiation. More importantly, in vivo bone defect repair experiments showed that new bone formed throughout the scaffolds at 60 days after implantation. All these results suggested that the PEEK-HAP/GO scaffolds have a promising potential for bone tissue engineering application.

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“…Due to the large surface area to volume ratio of electrospun scaffolds, cell adhesion, migration, and proliferation improve. [7][8][9] Various types of biomaterials such as natural polymers, synthetic polymers, ceramics, and their composites can be applied to the electrospinning process. The combination of natural and synthetic polymers improves mechanical properties as well as biocompatibility.…”

mentioning

confidence: 99%

In VitroandIn VivoStudies of BMP-2-Loaded PCL–Gelatin–BCP Electrospun Scaffolds

Kim

Linh

Min

et al. 2014

Tissue Engineering Part A

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To confirm the effect of recombinant human bone morphogenetic protein-2 (BMP-2) for bone regeneration, BMP-2-loaded polycaprolactone (PCL)-gelatin (Gel)-biphasic calcium phosphate (BCP) fibrous scaffolds were fabricated using the electrospinning method. The electrospinning process to incorporate BCP nanoparticles into the PCL-Gel scaffolds yielded an extracellular matrix-like microstructure that was a hybrid system composed of nano-and micro-sized fibers. BMP-2 was homogeneously loaded on the PCL-Gel-BCP scaffolds for enhanced induction of bone growth. BMP-2 was initially released at high levels, and then showed sustained release behavior for 31 days. Compared with the PCL-Gel-BCP scaffold, the BMP-2-loaded PCL-Gel-BCP scaffold showed improved cell proliferation and cell adhesion behavior. Both scaffold types were implanted in rat skull defects for 4 and 8 weeks to evaluate the biological response under physiological conditions. Remarkable bone regeneration was observed in the BMP-2/PCL-Gel-BCP group. These results suggest that BMP-2-loaded PCL-Gel-BCP scaffolds should be considered for potential bone tissue engineering applications.

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In vivo biocompatibility and osteogenesis of electrospun poly(ε-caprolactone)–poly(ethylene glycol)–poly(ε-caprolactone)/nano-hydroxyapatite composite scaffold

Cited by 106 publications

References 44 publications

Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs andIn VivoBone Formation by Activating Ca²⁺-Sensing Receptor Signaling

Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs andIn VivoBone Formation by Activating Ca²⁺-Sensing Receptor Signaling

Graphene oxide as an interface phase between polyetheretherketone and hydroxyapatite for tissue engineering scaffolds

In VitroandIn VivoStudies of BMP-2-Loaded PCL–Gelatin–BCP Electrospun Scaffolds

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In vivo biocompatibility and osteogenesis of electrospun poly(ε-caprolactone)–poly(ethylene glycol)–poly(ε-caprolactone)/nano-hydroxyapatite composite scaffold

Cited by 106 publications

References 44 publications

Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs andIn VivoBone Formation by Activating Ca2+-Sensing Receptor Signaling

Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs andIn VivoBone Formation by Activating Ca2+-Sensing Receptor Signaling

Graphene oxide as an interface phase between polyetheretherketone and hydroxyapatite for tissue engineering scaffolds

In VitroandIn VivoStudies of BMP-2-Loaded PCL–Gelatin–BCP Electrospun Scaffolds

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Product

Resources

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Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs andIn VivoBone Formation by Activating Ca²⁺-Sensing Receptor Signaling

Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs andIn VivoBone Formation by Activating Ca²⁺-Sensing Receptor Signaling