In Vivo Bioimaging Analysis of Stromal Vascular Fraction-Assisted Fat Grafting

Zhou, Shuang-Bai; Chiang, Cheng-An; Xie, Yun; Li, Hua; Li, Kai; Kobayashi, Eiji; Li, Qingfeng

doi:10.1097/tp.0000000000000397

Cited by 27 publications

(12 citation statements)

References 34 publications

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“…Fifteen studies were identified in which immunodeficient animals were used. One study used rats [ 40 ] and the remaining studies used mice [ 11 , 15 , 18 – 20 , 40 – 49 ]. Dosage for cell enrichment ranged from 5 × 10 5 to 2 × 10 7 cells/ml of lipoaspirate.…”

Section: Resultsmentioning

confidence: 99%

“…Dosage for cell enrichment ranged from 5 × 10 5 to 2 × 10 7 cells/ml of lipoaspirate. In all studies, human tissue and cells were used [ 11 , 18 – 20 , 40 – 47 ] except for three in which rat [ 49 ] or mice [ 15 , 48 ] tissue and cells were used. The relative survival improvement for simple CAL ranged from 1.19- to 2.37-fold compared with their respective control group, which consisted of regular lipotransfer.…”

Section: Resultsmentioning

confidence: 99%

“…One study did not measure survival rate [ 41 ]. Four studies systematically examined the morphology of the explanted fat grafts of which two found significantly better integrity and less cyst formation [ 40 , 42 , 43 , 49 ]. Many of the remaining studies described better morphology with more viable adipocytes, less necrosis, and cyst formation with CAL but they did not provide either sufficient description of their method or statistics to substantiate these claims.…”

Section: Resultsmentioning

confidence: 99%

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Cell-Assisted Lipotransfer: A Systematic Review of Its Efficacy

2016

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Introduction Autologous lipotransfer is seen as an ideal filler for soft tissue reconstruction. The main limitation of this procedure is the unpredictable resorption and volume loss of the fat graft. In the recent decade, an increasing amount of research has focused on the use of adipose tissue-derived stromal cells (ASCs) to enrich the fat graft, a procedure termed cell-assisted lipotransfer (CAL). The aim of this review was to systematically review the current preclinical and clinical evidence for the efficacy of CAL compared with conventional lipotransfer.Materials and MethodsA systematic search was performed on PubMed and other databases to identify all preclinical and clinical studies where CAL with ASCs was compared with conventional lipotransfer. A total of 20 preclinical studies and seven clinical studies were included in the review.ResultsThe preclinical studies consisted of 15 studies using immunodeficient animal models and five studies using immunocompetent studies. Seventeen studies examined weight/volume retention of which 15 studies favored CAL over conventional lipotransfer. One clinical study did not find any efficacy of CAL and the remaining six studies favored CAL.ConclusionsThe present evidence suggests that there is a big potential for CAL in reconstructive surgery; however, the present studies are so far still of low quality with inherent weaknesses. Several aspects regarding CAL still remain unknown such as the optimal degree of cell enrichment and also its safety. Further high-quality studies are needed to establish if CAL can live up to its potential.Level of Evidence VThis journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Cell-Assisted Lipotransfer: A Systematic Review of Its Efficacy

2016

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“…SVF obtained from liposuction is composed of many types cells, including mature fat cells, ASCs, endothelial cells, fibroblasts, and pericytes; furthermore, ASCs can differentiate to lipoblasts or vascular endothelial cells [ 29 – 34 ]. Recent studies have confirmed that SVF can facilitate the rapid vascularization of fat transplants and in turn increase survival rates [ 35 – 38 ]. Liposuction and the mechanical emulsification method generate nanofat with a diameter of approximately 50-100 μm that can help fat transplants, particularly the cells in the center, connect effectively to the ASCs and cell factors in SVF.…”

Section: Discussionmentioning

confidence: 99%

Nanofat-derived stem cells with platelet-rich fibrin improve facial contour remodeling and skin rejuvenation after autologous structural fat transplantation

Wei

Liang

et al. 2017

Oncotarget

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Traditional autologous fat transplantation is a common surgical procedure for treating facial soft tissue depression and skin aging. However, the transplanted fat is easily absorbed, reducing the long-term efficacy of the procedure. Here, we examined the efficacy of nanofat-assisted autologous fat structural transplantation. Nanofat-derived stem cells (NFSCs) were isolated, mechanically emulsified, cultured, and characterized. Platelet-rich fibrin (PRF) enhanced proliferation and adipogenic differentiation of NFSCs in vitro. We then compared 62 test group patients with soft tissue depression or signs of aging who underwent combined nanofat, PRF, and autologous fat structural transplantation to control patients (77 cases) who underwent traditional autologous fat transplantation. Facial soft tissue depression symptoms and skin texture were improved to a greater extent after nanofat transplants than after traditional transplants, and the nanofat group had an overall satisfaction rate above 90%. These data suggest that NFSCs function similarly to mesenchymal stem cells and share many of the biological characteristics of traditional fat stem cell cultures. Transplants that combine newly-isolated nanofat, which has a rich stromal vascular fraction (SVF), with PRF and autologous structural fat granules may therefore be a safe, highly-effective, and long-lasting method for remodeling facial contours and rejuvenating the skin.

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“…[30][31][32] However, the findings of Zhou et al showed a positive interaction between the implanted stromal vascular fraction cells and the surrounding fat tissue. 33 We concluded that, in our experiment, bone marrow concentrate cells might be dealing with BMA, bone marrow aspirate; BMC, bone marrow concentrate; PBS, phosphate-buffered saline; NS, not significant. *Data are presented in terms of mean ± SD.…”

Section: Discussionmentioning

confidence: 88%