In vivo bioluminescence imaging of locally disseminated colon carcinoma in rats

Zeamari, S; Rumping, Gaby; Floot, Ben; Lyons, Scott K.; Stewart, Fiona

doi:10.1038/sj.bjc.6601637

Cited by 42 publications

(26 citation statements)

References 15 publications

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“…Commercially available BLI only collects the tumor signal in a surface-weighted manner on a two-dimensional plane (13,34), which means a weaker source near the surface would have identical light intensity with a stronger source in deep tissue (35). Therefore, we tried to monitor the progression of gastric tumors by home-developed BLT system for the first time.…”

Section: Discussionmentioning

confidence: 99%

“…Bioluminescence imaging (BLI), especially, demonstrates the most desirable combination of high sensitivity and easy handling (5) and is suitable for acquire signal deep in tissue (6). It has been widely used for monitoring different cancer biological processes [angiogenesis (7,8), infinite proliferation and meta stasis (9,10), multidrug resistance (11,12)] and assessing the efficacy of different therapeutic modalities (13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

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Real-time bioluminescence and tomographic imaging of gastric cancer in a novel orthotopic mouse model

Liu

Yao

et al. 2012

Oncol Rep

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Abstract. Gastric cancer is the second leading cause of cancer mortality worldwide. Understanding the multistep process of carcinogenesis of gastric cancer is pivotal to develop novel therapeutic strategies. Molecular imaging in preclinical cancer models bridges the gap of laboratory-based experiment and clinical translation. To this end, the human gastric cancer cell line SGC-7901 was established to stably express luciferase and GFP by lentiviral transduction (SGC7901-Luc-GFP). Preclinical models were developed by orthotopic transplantation of SGC-7901-Luc-GFP into the sub-serosal layer of the stomach of immunocompromised mice. Tumor progression and therapeutic responses were dynamically tracked by bioluminescence imaging (BLI). Bioluminescence tomography (BLT) was used to monitor stereoscopic morphological and signal changes during tumor progression. Good correlation between cell number and bio luminescence/ fluorescence intensity was observed (R 2 =0.9983/r 2 =0.9974) in vitro. Tumor progression and therapeutic response could be successfully followed directly by BLI. Importantly, BLT provided a more accurate spatial location and tomographic quantification of the internal lesion. In conclusion, our novel bioluminescence-based preclinical gastric cancer models enable superior, noninvasive monitoring gastric cancer progression and their drug responses. The BLT technique in particular, may have great potential for future oncological studies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Real-time bioluminescence and tomographic imaging of gastric cancer in a novel orthotopic mouse model

Liu

Yao

et al. 2012

Oncol Rep

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“…In vivo bioluminescence imaging has also been shown to be an invaluable tool for monitoring and studying tumour development in animal models (8,9). It has been successfully used to follow many different types of tumours, such as prostate, breast, colon, ovarian and lung cancer (10)(11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

The expression level of luciferase within tumour cells can alter tumour growth upon in vivo bioluminescence imaging

et al. 2007

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In vivo bioluminescence imaging is becoming a very important tool for the study of a variety of cellular and molecular events or disease processes in living systems. In vivo bioluminescence imaging is based on the detection of light emitted from within an animal. The light is generated as a product of the luciferase-luciferin reaction taking place in a cell. In this study, we implanted mice with tumour cells expressing either a high or a low level of luciferase. In vivo bioluminescence imaging was used to follow tumour progression. Repeated luciferin injection and imaging of high and low luciferase-expressing tumours was performed. While low luciferase-expressing tumours grew similarly to vector controls, growth of the high luciferase-expressing tumours was severely inhibited. The observation that a high level of luciferase expression will inhibit tumour cell growth when an animal is subjected to serial in vivo bioluminescence imaging is potentially an important factor in designing these types of studies.

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“…Transfection of immune or hematopoietic cells with fluorescent or luminescent reporter genes ͑for example, luciferase, green fluorescent protein, or red fluorescent protein͒ prior to their injection is a technically complicated procedure giving relatively low yield that is difficult to standardize. 9,10 Recently, much attention has been drawn to organic dyes and quantum dots ͑QDs͒, NIR light, allowing photons to penetrate up to several centimeters into living tissue. [11][12][13] Despite optical advantages of QDs compared to organic dyes, there are still several limitations and concerns regarding the use of QD-bioconjugates for in vivo cellular imaging.…”

mentioning

confidence: 99%

Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing

Kalchenko¹,

et al. 2006

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Abstract.We develop an optical whole-body imaging technique for monitoring normal and leukemic hematopoietic cell homing in vivo. A recently developed nearinfrared ͑NIR͒ lipophilic carbocyanine dye 1,1Ј-dioctadecyl-3 ,3,3Ј ,3Ј-tetramethylindotricarbocyanine iodide ͑DiR͒ is used to safely and directly label the membranes of human leukemic Pre-B ALL G2 cell lines as well as primary murine lymphocytes and erythrocytes. DiR has absorption and fluorescence maxima at 750 and 782 nm, respectively, which corresponds to low light absorption and autofluorescence in living tissues. This allows us to obtain a significant signal with very low background level. A charge-coupled device ͑CCD͒-based imager is used for noninvasive whole-body imaging of DiR-labeled cell homing in intact animals. This powerful technique can potentially visualize any cell type without use of specific antibodies conjugated with NIR fluorescent tag or loading cells with transporter-delivered NIR fluorophores. Thus, in vivo imaging based on NIR lipophilic carbocyanine dyes in combination with advanced optical techniques may serve as a powerful alternative or complementation to other small animal imaging methods. Noninvasive, in vivo imaging of targeted cells is a promising field for studying cell behavior and movement in animal models. Intravital microscopy and whole-body fluorescent optical imaging are among the most widely used approaches for visualizing naive or genetically modified cells in vivo.

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In vivo bioluminescence imaging of locally disseminated colon carcinoma in rats

Cited by 42 publications

References 15 publications

Real-time bioluminescence and tomographic imaging of gastric cancer in a novel orthotopic mouse model

Real-time bioluminescence and tomographic imaging of gastric cancer in a novel orthotopic mouse model

The expression level of luciferase within tumour cells can alter tumour growth upon in vivo bioluminescence imaging

Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing

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