In vivo biosynthesis of long-chain polyunsaturated fatty acids by the euryhaline rotifer (Brachionus plicatilis)

Pérez, Jose Antonio Pérez; Reis, Diana B.; Ramírez, Daniel; Acosta, N.G.; Dorta‐Guerra, Roberto; Jeréz, S.; Rodrı́guez, Covadonga

doi:10.1016/j.aquaculture.2022.738415

Cited by 6 publications

(1 citation statement)

References 60 publications

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“…Evidence supporting the capacity of invertebrates for LC-PUFA biosynthesis had been mostly collected from compositional studies and biochemical assays aiming to determine in vivo bioconversions of labelled fatty acid substrates [25][26][27][28][29][30][31]. However, the ever-increasing availability of genomic data from multiple invertebrates now offers the opportunity to identify the specific genes encoding LC-PUFA biosynthetic enzymes, thus often enabling one to distinguish the invertebrate's enzymatic capacity from that of (micro)organisms that may inhabit their hosts [32].…”

Section: Introductionmentioning

confidence: 99%

Examination of gammarid transcriptomes reveals a widespread occurrence of key metabolic genes from epibiont bdelloid rotifers in freshwater species

Ribes-Navarro,

Kabeya,

Castro

et al. 2023

Open Biol.

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Previous data revealed the unexpected presence of genes encoding for long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic enzymes in transcriptomes from freshwater gammarids but not in marine species, even though closely related species were compared. This study aimed to clarify the origin and occurrence of selected LC-PUFA biosynthesis gene markers across all published gammarid transcriptomes. Through systematic searches, we confirmed the widespread occurrence of sequences from seven elongases and desaturases involved in LC-PUFA biosynthesis, in transcriptomes from freshwater gammarids but not marine species, and clarified that such occurrence is independent from the gammarid species and geographical origin. The phylogenetic analysis established that the retrieved elongase and desaturase sequences were closely related to bdelloid rotifers, confirming that multiple transcriptomes from freshwater gammarids contain contaminating rotifers' genetic material. Using the Adineta steineri genome, we investigated the genomic location and exon–intron organization of the elongase and desaturase genes, establishing they are all genome-anchored and, importantly, identifying instances of horizontal gene transfer. Finally, we provide compelling evidence demonstrating Bdelloidea desaturases and elongases enable these organisms to perform all the reactions for de novo biosynthesis of PUFA and, from them, LC-PUFA, an advantageous trait when considering the low abundance of these essential nutrients in freshwater environments.

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Section: Introductionmentioning

confidence: 99%

Examination of gammarid transcriptomes reveals a widespread occurrence of key metabolic genes from epibiont bdelloid rotifers in freshwater species

Ribes-Navarro,

Kabeya,

Castro

et al. 2023

Open Biol.

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Antioxidant capacity and lipid composition of Brachionus plicatilis and Artemia enriched with a mixture of different post-processing formats of Navicula salinicola and Isochrysis galbana and lipid emulsions

Galindo,

Pérez,

Almansa

et al. 2024

J Appl Phycol

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Live prey used by the aquaculture industry are usually poor in some essential nutrients including long chain polyunsaturated fatty acids (LC-PUFA) and must be enriched to improve their nutritional value prior to larval feeding. Standard enrichment protocols are commonly based on lipid emulsions, being associated to a high oxidative stress condition. The combination of microalgae and lipid emulsion can palliate this situation, where the oxidative stress can be partially compensated by the antioxidant compounds present in microalgae. The maintenance of living microalgae in culture facilities is laborious, and the produced biomass may present fluctuating properties, leading to a serious bottleneck in the cultivation of live prey. Hence, substitutes for live microalgae including pastes or dried formats are receiving increasing research attention due to its nutritional stability, longer shelf-life and easy handling. In this study four different microalgae formats combined with a lipid emulsion are tested as enrichment products for Brachionus plicatilis and Artemia. Thus, fresh, frozen and spray-dried Navicula salinicola (NFRE, NFRO and NSD, respectively), and spray-dried Isochrysis galbana (ISD) were mixed with a commercial oil concentrate (IncromegaTM) or a marine lecithin (LC 60®), and added for 5 h to the rotifer or Artemia culture media. The antioxidant capacity of the microalgae extracts and the live prey activity of antioxidant enzymes, peroxides index (PxI) and thiobarbituric acid reactive substances (TBARS) were evaluated. The lipid profile of microalgae formats and enriched live preys was also determined. Ethyl acetate extract was the most antioxidant active extract of all microalgae formats. In addition, overall, I. galbana seems to be better than any N. salinicola format for a more effective protection against oxidative stress and for live prey lipid enrichment. Both rotifer and Artemia cultured with the mixture of I. galbana and the lipid emulsion generally showed higher DHA/EPA and EPA/ARA ratios. Moreover, the combination of the microalgae with LC 60® lipid emulsion highly favored Artemia´s polar lipid and DHA incorporation. Among microalgae products, both spray-dried formats better enhanced live prey n-3 LC-PUFA content. Our results highlight the great potential of new microalgae-derived products to improve effectiveness of current live prey lipid enrichment protocols used in aquaculture.

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L-carnitine can promote the growth performance of rotifers (Brachionus rotundiformis) by improving their feeding behavior and nutrient composition

Gao,

Liu,

Zhang

et al. 2024

Aquaculture Reports

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In vivo biosynthesis of long-chain polyunsaturated fatty acids by the euryhaline rotifer (Brachionus plicatilis)

Cited by 6 publications

References 60 publications

Examination of gammarid transcriptomes reveals a widespread occurrence of key metabolic genes from epibiont bdelloid rotifers in freshwater species

Examination of gammarid transcriptomes reveals a widespread occurrence of key metabolic genes from epibiont bdelloid rotifers in freshwater species

Antioxidant capacity and lipid composition of Brachionus plicatilis and Artemia enriched with a mixture of different post-processing formats of Navicula salinicola and Isochrysis galbana and lipid emulsions

L-carnitine can promote the growth performance of rotifers (Brachionus rotundiformis) by improving their feeding behavior and nutrient composition

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