In Vivo Biotinylated scFv Fragments

Single-chain antibody fragments: Purification methodologies

“…A cheaper version of this tag was more recently tested by Lan et al in expanded bed adsorption [48], with recovery yields of more than 85%. Another example of scFv purification by affinity stretches is the expression of biotinylated scFv and its ulterior purification with the natural biotin ligand avidine/streptavidin [49].…”