In vivo bone regeneration with injectable chitosan/hydroxyapatite/collagen composites and mesenchymal stem cells

Huang, Zhi Xiong; Chen, Yan; Feng, Qingling; Zhao, Wei; Yu, Bo; Tian, Jinhui; Li, Songjian; Lin, Bomiao

doi:10.1007/s11706-011-0142-4

Cited by 17 publications

(13 citation statements)

References 39 publications

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“…The CS/nHAC composite was prepared by the procedure reported previously [24][25][26][27]. nHAC powder was synthesized by self-assembly of nanofibrils of mineralized collagen and sterilized by γ-ray irradiation (1.5 Mrad).…”

Section: Scaffold Processingmentioning

confidence: 99%

“…Overall, there was not a significant amount of cell proliferation detected over the course of the study. In vitro cell testing of biomaterials is a well-established method to determine the cytocompatibility of the materials [27][28][29] by using either cell lines or primary cells. This study was designed primarily to analyze the ability of the composite scaffold to support osteoblast matrix production, and maintaining similar cell numbers in each group allowed differences in these parameters to be studied without the confounding effects of cell number differences.…”

Section: Cell Proliferation By Dna Quantificationmentioning

confidence: 99%

“…In the previouse study, the feasibility of developing a thermosensitive and injectable chitosan 2 Journal of Nanomaterials solution in the presence of nHAC was demonstrated [23][24][25]. Combining nHAC and chitosan has the potential to maximize the beneficial properties of each and creates an injectable scaffold with properties similar to physiological bone which would undoubtedly aid in the formation of new bone at the tissue/biomaterial interface [24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable Chitosan/Nano‐Hydroxyapatite/Collagen Scaffold

Chen

Huang

et al. 2012

Journal of Nanomaterials

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The purpose of this study was to evaluate the in vitro cell biocompatibility of an in situ forming composite consisting of chitosan (CS), nano-hydroxyapatite and collagen (nHAC), which has a complex hierarchical structure similar to natural bone. MC3T3-E1 mouse calvarial preosteoblasts were cultured on the surface of the injectable CS/nHAC and CS scaffold. The proliferations of seeded MC3T3-E1 were investigated for 10 days. Cytotoxicity, cell proliferation, and cell expression of osteogenic markers such as alkaline phosphatase (ALP), type 1 collagen (COL-1), RUNX-2, and osteocalcin (OCN) were examined by biochemical assay and reverse transcription polymerase chain reaction. Cell viability and total cellularity (measured by dsDNA) were similar between the two scaffold groups. However, ALP, COL-1, OCN, and RUNX-2 production were significantly greater when osteoblasts were cultured on CS/nHAC scaffolds. The increase in osteogenic markers production on CS/nHAC scaffolds indicated that these scaffolds were superior to chitosan-only scaffolds in facilitating osteoblast mineralization. These results demonstrate the potential of the CS/nHAC scaffolds to be used in bone tissue engineering.

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Section: Scaffold Processingmentioning

confidence: 99%

Section: Cell Proliferation By Dna Quantificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable Chitosan/Nano‐Hydroxyapatite/Collagen Scaffold

Chen

Huang

et al. 2012

Journal of Nanomaterials

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“…Since for reconstruction of irregular bone defects injectable biomaterials are more appropriate than preformed biomaterials, the development of a biomimetic in situ forming composite consisting of chitosan and mineralized collagen fibrils is required. Such chitosan and mineralized collagen fibrils which have a complex hierarchical structure similar to natural bone were obtained and their biological properties were studied …”

Section: Collagen/chitosan Compositesmentioning

confidence: 99%

Biopolymeric nanocomposites for potential biomedical applications

Sionkowska

2016

Polymer International

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This review reports recent advances in the field of biopolymeric composites and nanocomposites for potential biomedical applications. These materials have attracted both academic and, for several composites, industrial attention because they exhibit properties required in the biomedical field. Herein, the structure, preparation and properties of biopolymeric composite blends are discussed in general, and detailed examples are also drawn from the scientific literature and practical work. In this review the most common natural polymers collagen, chitosan and their composites and nanocomposites with inorganic particles are discussed.

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“…NHAC uniformly dispersed in the chitosan matrix and this CS/nHAC composite showed some features of natural bone. Thus, the CS/nHAC scaffolds have shown to possess promising physicochemical and biological properties for use in bone tissue engineering approaches [23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Noninvasive Evaluation of Injectable Chitosan/Nano‐Hydroxyapatite/Collagen Scaffold via Ultrasound

Chen

et al. 2012

Journal of Nanomaterials

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To meet the challenges of designing anin situforming scaffold and regenerating bone with complex three-dimensional (3D) structures, anin situforming hydrogel scaffold based on nano-hydroxyapatite (nHA), collagen (Col), and chitosan (CS) was synthesized. Currently, only a limited number of techniques are available to mediate and visualize the injection process of the injectable biomaterials directly and noninvasively. In this study, the potential of ultrasound for the quantitativein vivoevaluation of tissue development in CS/nHAC scaffold was evaluated. The CS/nHAC scaffold was injected into rat subcutaneous tissue and evaluated for 28 days. Quantitative measurements of the gray-scale value, volume, and blood flow of the scaffold were evaluated using diagnostic technique. This study demonstrates that ultrasound can be used to noninvasively and nondestructively monitor and evaluate thein vivocharacteristics of injectable bone scaffold. In comparison to the CS, the CS/nHAC scaffold showed a greater stiffness, less degradation rate, and better blood supply in thein vivoevaluation. In conclusion, the diagnostic ultrasound method is a good tool to evaluate thein vivoformation of injectable bone scaffolds and facilitates the broad use to monitor tissue development and remodeling in bone tissue engineering.

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In vivo bone regeneration with injectable chitosan/hydroxyapatite/collagen composites and mesenchymal stem cells

Cited by 17 publications

References 39 publications

In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable Chitosan/Nano‐Hydroxyapatite/Collagen Scaffold

In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable Chitosan/Nano‐Hydroxyapatite/Collagen Scaffold

Biopolymeric nanocomposites for potential biomedical applications

Noninvasive Evaluation of Injectable Chitosan/Nano‐Hydroxyapatite/Collagen Scaffold via Ultrasound

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