In vivo cardiovasculogenesis by direct injection of isolated adult mesenchymal stem cells

Gojo, Satoshi; Gojo, Noriko; Takeda, Yukiji; Mori, Taisuke; Abe, Hitoshi; Kyo, Shunei; Hata, Junichi; Umezawa, Akihiro

doi:10.1016/s0014-4827(03)00132-0

Cited by 237 publications

(151 citation statements)

References 35 publications

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“…However, similar to MSCs, these cells were able to differentiate into adipocytes and osteocytes, suggesting that they are a higher cell lineage than cartilagecommitted progenitor cells, which are predetermined to form chondrocytes of elastic cartilage. In line with these expectations, CSPCs expressed CD44 and CD90, similar to MSCs, whereas they did not express the MSC markers CD133, CD140a, CD146, or CD271 (24,33,34). Thus, our isolated stem/progenitor cells are closely related to MSCs, yet are a distinct cartilage stem/ progenitor cell population.…”

Section: Discussionsupporting

confidence: 82%

Reconstruction of human elastic cartilage by a CD44 ⁺ CD90 ⁺ stem cell in the ear perichondrium

Kobayashi

Takebe²,

Inui³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Despite the great demands for treating craniofacial injuries or abnormalities, effective treatments are currently lacking. One promising approach involves human elastic cartilage reconstruction using autologous stem/progenitor populations. Nevertheless, definitive evidence of the presence of stem cells in human auricular cartilage remains to be established. Here, we demonstrate that human auricular perichondrium, which can be obtained via a minimally invasive approach, harbors a unique cell population, termed as cartilage stem/progenitor cells (CSPCs). The clonogenic progeny of a single CD44 + CD90 + CSPC displays a number of features characteristic of stem cells. Highly chondrogenic CSPCs were shown to reconstruct large (>2 cm) elastic cartilage after extended expansion and differentiation. CSPC-derived cartilage was encapsulated by a perichondrium layer, which contains a CD44 + CD90 + self-renewing stem/progenitor population and was maintained without calcification or tumor formation even after 10 mo. This is a unique report demonstrating the presence of stem cells in auricular cartilage. Utilization of CSPCs will provide a promising reconstructive material for treating craniofacial defects with successful long-term tissue restoration.

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Section: Discussionsupporting

confidence: 82%

Reconstruction of human elastic cartilage by a CD44 ⁺ CD90 ⁺ stem cell in the ear perichondrium

Kobayashi

Takebe²,

Inui³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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“…95 Furthermore, MSC and MAPC were reported to be also 'plastic' in vivo and able to regenerate several organs in animal models (eg heart, spinal cord, liver). [98][99][100] Recently, however, some doubts accumulated around the concept of marrow-derived fibroblastic cells being 'plastic' precursors of versatile tissue-specific stem cells. First, some elegant studies demonstrated that the neural differentiation of fibroblasts is probably an in vitro artifact that depends on morphological changes of these cells induced during their culture in neural-differentiating media.…”

Section: Heterogenous Populations Of Stem Cells In Bone Marrowmentioning

confidence: 99%

Bone marrow as a home of heterogenous populations of nonhematopoietic stem cells

et al. 2005

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Evidence is presented that bone marrow (BM) in addition to CD45 positive hematopoietic stem cells contains a rare population of heterogenous CD45 negative nonhematopoietic tissue committed stem cells (TCSC). These nonhematopoietic TCSC (i) are enriched in population of CXCR4 þ CD34 þ AC133 þ lin À CD45 À and CXCR4 þ Sca-1 þ lin À CD45 À in humans and mice, respectively, (ii) display several markers of pluripotent stem cells (PSC) and (iii) as we envision are deposited in BM early in development. Thus, since BM contains versatile nonhematopoietic stem cells, previous studies on plasticity trans-dedifferentiation of BM-derived hematopoietic stem cells (HSC) that did not include proper controls to exclude this possibility could lead to wrong interpretations. Therefore, in this spotlight review we present this alternative explanation of 'plasticity' of BMderived stem cells based on the assumption that BM stem cells are heterogenous. We also discuss a potential relationship of TCSC/PSC identified by us with other BM-derived CD45 negative nonhematopoietic stem cells that were recently identified by other investigators (eg MSC, MAPC, USSC and MIAMI cells). Finally, we discuss perspectives and pitfalls in potential application of these cells in regenerative medicine.

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“…Additionally, studies have shown that MSCs have minimal immunogenic response in vivo, making them potentially suitable for allogeneic transplantation into human patients (5). Previous work has shown that when MSCs are transplanted into the heart, they can differentiate into SMCs and contribute to vascular remodeling (6,7), suggesting that certain microenvironmental cues may be important in promoting differentiation to a SMC phenotype. However, the effects of vascular microenvironmental factors on MSCs are not yet well understood.…”

mentioning

confidence: 99%

Anisotropic mechanosensing by mesenchymal stem cells

Kurpinski

Chu

Hashi

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

363

316

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Mesenchymal stem cells (MSCs) are a potential source for the construction of tissue-engineered vascular grafts. However, how vascular mechanical forces regulate the genetic reprogramming in MSCs is not well understood. Mechanical strain in the vascular wall is anisotropic and mainly in the circumferential direction. We have shown that cyclic uniaxial strain on elastic substrates causes the cells to align perpendicularly to the strain axis, which is different from that in the vascular wall. To simulate the vascular cell alignment and investigate the anisotropic mechanical sensing by MSCs, we used soft lithography to create elastomeric membranes with parallel microgrooves. This topographic pattern kept MSCs aligned parallel to the strain axis, and the cells were subjected to 5% cyclic uniaxial strain (1 Hz) for 2-4 days. DNA microarray analysis revealed global gene expression changes, including an increase in the smooth muscle marker calponin 1, decreases in cartilage matrix markers, and alterations in cell signaling (e.g., down-regulation of the Jagged1 signaling pathway). In addition, uniaxial strain increased MSC proliferation. However, when micropatterning was used to align cells perpendicularly to the axis of mechanical strain, the changes of some genes were diminished, and MSC proliferation was not affected. This study suggests that mechanical strain plays an important role in MSC differentiation and proliferation, and that the effects of mechanotransduction depend on the orientation of cells with respect to the strain axis. The differential cellular responses to the anisotropic mechanical environment have important implications in cardiovascular development, tissue remodeling, and tissue engineering. stem cell engineering ͉ differentiation ͉ genetic reprogramming ͉ uniaxial mechanical strain ͉ micropatterning

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In vivo cardiovasculogenesis by direct injection of isolated adult mesenchymal stem cells

Cited by 237 publications

References 35 publications

Reconstruction of human elastic cartilage by a CD44 ⁺ CD90 ⁺ stem cell in the ear perichondrium

Reconstruction of human elastic cartilage by a CD44 ⁺ CD90 ⁺ stem cell in the ear perichondrium

Bone marrow as a home of heterogenous populations of nonhematopoietic stem cells

Anisotropic mechanosensing by mesenchymal stem cells

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In vivo cardiovasculogenesis by direct injection of isolated adult mesenchymal stem cells

Cited by 237 publications

References 35 publications

Reconstruction of human elastic cartilage by a CD44 + CD90 + stem cell in the ear perichondrium

Reconstruction of human elastic cartilage by a CD44 + CD90 + stem cell in the ear perichondrium

Bone marrow as a home of heterogenous populations of nonhematopoietic stem cells

Anisotropic mechanosensing by mesenchymal stem cells

Contact Info

Product

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Reconstruction of human elastic cartilage by a CD44 ⁺ CD90 ⁺ stem cell in the ear perichondrium

Reconstruction of human elastic cartilage by a CD44 ⁺ CD90 ⁺ stem cell in the ear perichondrium