In Vivo Cell Tracking with Bioluminescence Imaging

Kim, Jung Eun; Kalimuthu, S; Ahn, Byeong‐Cheol

doi:10.1007/s13139-014-0309-x

Cited by 136 publications

(129 citation statements)

References 89 publications

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“…In particular, the adapted bioluminescence reporters, such as Gaussia luciferase, being over 1,000-fold brighter than firefly luciferase, are useful tools to study temporal properties of minute biological processes because of their sensitivity, low background and independence from an excitation source to emit light. Therefore, BLI has been extensively evaluated in the development of cell-based therapies to determine cellular distribution, survival, proliferation, and differentiation after transplantation [107, 108]. BLI has also been described for the analysis of EVs associated with a luciferase enzyme.…”

Section: Investigating Extracellular Vesicles Biodistribution By Mmentioning

confidence: 99%

Towards Therapeutic Delivery of Extracellular Vesicles: Strategies for In Vivo Tracking and Biodistribution Analysis

Rocco¹,

Baldari²,

Toietta³

2016

Stem Cells International

120

101

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Extracellular vesicles (EVs), such as microvesicles and exosomes, are membranous structures containing bioactive material released by several cells types, including mesenchymal stem/stromal cells (MSCs). Increasing lines of evidences point to EVs as paracrine mediators of the beneficial effects on tissue remodeling associated with cell therapy. Administration of MSCs-derived EVs has therefore the potential to open new and safer therapeutic avenues, alternative to cell-based approaches, for degenerative diseases. However, an enhanced knowledge about in vivo EVs trafficking upon delivery is required before effective clinical translation. Only a few studies have focused on the biodistribution analysis of exogenously administered MSCs-derived EVs. Nevertheless, current strategies for in vivo tracking in animal models have provided valuable insights on the biodistribution upon systemic delivery of EVs isolated from several cellular sources, indicating in liver, spleen, and lungs the preferential target organs. Different strategies for targeting EVs to specific tissues to enhance their therapeutic efficacy and reduce possible off-target effects have been investigated. Here, in the context of a possible clinical application of MSC-derived EVs for tissue regeneration, we review the existing strategies for in vivo tracking and targeting of EVs isolated from different cellular sources and the studies elucidating the biodistribution of exogenously administered EVs.

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Section: Investigating Extracellular Vesicles Biodistribution By Mmentioning

confidence: 99%

Towards Therapeutic Delivery of Extracellular Vesicles: Strategies for In Vivo Tracking and Biodistribution Analysis

Rocco¹,

Baldari²,

Toietta³

2016

Stem Cells International

120

101

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“…Due to its spectral characteristics and convenience to perform, this technique is widely used to track cancer metastasis and the effects of antineoplastic therapy in animal models (Kim et al, 2015). In the present study, the in vivo antitumor efficacy of MWCNTs/DOX/TC was investigated by assessing the radiance efficiency of luciferase-expressing BEL7402 cells with the change of tumor volume.…”

Section: Assessment Of In Vitro Antitumor Activitymentioning

confidence: 99%

Simultaneous monitoring of the drug release and antitumor effect of a novel drug delivery system-MWCNTs/DOX/TC

et al. 2017

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Monitoring drug release and therapeutic efficacy is crucial for developing drug delivery systems. Our preliminary study demonstrated that, as compared with pristine multiwalled carbon nanotubes (MWCNTs), transactivator of transcription (TAT)-chitosan functionalized MWCNTs (MWCNTs-TC) were a more promising candidate for drug delivery in cancer therapy. In the present study, a MWCNTs/TC-based drug delivery system was developed for an anticancer drug, doxorubicin (DOX). The drug loading and in vitro release profiles, cellular uptake and cytotoxicity were assessed. More importantly, the in vivo drug release and antitumor effect of MWCNTs/DOX/TC were evaluated by noninvasive fluorescence and bioluminescence imaging. It was demonstrated that MWCNTs/DOX/TC can be efficiently taken up by BEL-7402 hepatoma cells. The release of DOX from MWCNTs/DOX/TC was faster under lower pH condition, which was beneficial for intrcellular drug release. The in vivo release process of DOX and antitumor effect in animal model were monitored simultaneously by noninvasive fluorescence and luminescence imaging, which demonstrated the application potential of MWCNTs/DOX/TC for cancer therapy.

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“…Their reactions do not require ATP and oxygen, but are limited by substrate penetration and light absorption; Thus, unfortunately, they are rarely utilized for in vivo studies [41,42]. To improve the in vivo imaging performance of Renilla luciferases Loening et al and Rahnama et al have engineered variants with a red-shifted spectrum (peak emission of 556 and 540nm), of which a substantial portion is above 600nm [77,78].…”

Section: Reporter Genes For Bioluminescence Imagingmentioning

confidence: 99%

New imaging probes to track cell fate: reporter genes in stem cell research

Jurgielewicz

Harmsen

Wei

et al. 2017

Cell. Mol. Life Sci.

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Cell fate is a concept used to describe the differentiation and development of a cell in its organismal context over time. It is important in the field of regenerative medicine, where stem cell therapy holds much promise but is limited by our ability to assess its efficacy, which is mainly due to the inability to monitor what happens to the cells upon engraftment to the damaged tissue. Currently, several imaging modalities can be used to track cells in the clinical setting; however, they do not satisfy many of the criteria necessary to accurately assess several aspects of cell fate. In recent years, reporter genes have become a popular option for tracking transplanted cells, via various imaging modalities in small mammalian animal models. This review article examines the reporter gene strategies used in imaging modalities such as MRI, SPECT/PET, Optoacoustic and Bioluminescence Imaging. Strengths and limitations of the use of reporter genes in each modality are discussed.

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In Vivo Cell Tracking with Bioluminescence Imaging

Cited by 136 publications

References 89 publications

Towards Therapeutic Delivery of Extracellular Vesicles: Strategies for In Vivo Tracking and Biodistribution Analysis

Towards Therapeutic Delivery of Extracellular Vesicles: Strategies for In Vivo Tracking and Biodistribution Analysis

Simultaneous monitoring of the drug release and antitumor effect of a novel drug delivery system-MWCNTs/DOX/TC

New imaging probes to track cell fate: reporter genes in stem cell research

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