In Vivo Chaperone‐Assisted Folding of <i>α</i>‐1,6‐Fucosyltransferase from <i>Rhizobium</i> sp.

Bastida, Ágatha; Latorre, Montserrat; García‐Junceda, Eduardo

doi:10.1002/cbic.200200514

ChemBioChem

2003

DOI: 10.1002/cbic.200200514

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In Vivo Chaperone‐Assisted Folding of α‐1,6‐Fucosyltransferase from Rhizobium sp.

Ágatha Bastida

Montserrat Latorre

Eduardo García‐Junceda

Abstract: Three is not a crowd: Coexpression of the chaperonins GroEL/GroES with the α‐1,6‐fucosyltransferase from Rhizobium sp. allows a 10‐fold increase in production of soluble and active fucosyltransferase. The His‐tagged fucosyltransferase can be purified and immobilized on Ni2+‐IDA‐agarose gel in only one step (see Scheme). The immobilization leads to an important stabilization of the recombinant enzyme, which allows the preparation of a robust biocatalyst for the synthesis of oligosaccharides.

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Cited by 4 publications

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15 Bioinspired organic chemistry

Williams¹

2004

Annu. Rep. Prog. Chem., Sect. B: Org. Chem.

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The interface between chemistry and biology has become increasingly blurred as biological processes are understood in molecular terms and they enter the domain that is perhaps more accurately described as chemistry. This provides inspiration for creating new molecular systems and greater insight into how to reproduce similar processes through de novo designs. This article reviews the literature from 2003 in areas involving organic chemistry that have been inspired in some way by the form, function or methods found in biological systems.

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15 Bioinspired organic chemistry

Williams¹

2004

Annu. Rep. Prog. Chem., Sect. B: Org. Chem.

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Synthesis and evaluation of xylopyranoside derivatives as “decoy acceptors” of human β-1,4-galactosyltransferase 7

et al. 2011

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Proteoglycans (PGs), including heparan sulfate forms, are important regulators of tumor progression. In the PGs biosynthetic process, the core protein is synthesized on a ribosomal template and the sugar chains are assembled post-translationally, one sugar at a time, starting with the linkage of xylose to a serine residue of the core protein and followed by galactosidation of the xylosylprotein. Hydrophobic xylopyranosides have been previously shown to prime heparan sulfate synthesis, a property that was required to cause growth inhibition of tumor cells. To know if the antiproliferative activity of synthetic xylopyranosides is related to their ability to act as "decoy acceptors" of xylosylprotein 4-β-galactosyltransferase, we have heterologously expressed the catalytic domain of the human β-1,4-GalT 7 and studied the ability of a variety of synthetic xylopyranoside derivatives to act as substrates or inhibitors of the recombinant enzyme.

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Bacterial symbionts induce a FUT2-dependent fucosylated niche on colonic epithelium via ERK and JNK signaling

Meng¹,

Newburg

Young

et al. 2007

American Journal of Physiology-Gastrointestinal and Liver Physi

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The intestinal epithelium of the adult gut supports a complex, dynamic microbial ecosystem and expresses highly fucosylated glycans on its surface. Uncolonized gut contains little fucosylated glycan. The transition toward adult colonization, such as during recovery from germ-free status or from antibiotic treatment, increased expression of fucosylated epitopes in the colonic epithelium. This increase in fucosylation is accompanied by induction of fut2 mRNA expression and alpha1,2/3-fucosyltransferase activity. Colonization stimulates ERK and JNK signal transduction pathways, resulting in activation of transcription factors ATF2 and c-Jun, respectively. This increases transcription of fut2 mRNA and expression of alpha1,2/3-fucosyltransferase activity, resulting in a highly fucosylated intestinal mucosa characteristic of the adult mammalian gut. Blocking the ERK and JNK signaling cascade inhibits the ability of colonization to induce elevated fut2 mRNA and fucosyltransferase activity in the mature colon. Thus pioneer-mutualist symbiotic bacteria may utilize the ERK and JNK signaling cascade to induce the high degree of fucosylation characteristic of adult mammalian colon, and we speculate that this fucosylation facilitates colonization by adult microbiota.

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In Vivo Chaperone‐Assisted Folding of α‐1,6‐Fucosyltransferase from Rhizobium sp.

Cited by 4 publications

References 16 publications

15 Bioinspired organic chemistry

15 Bioinspired organic chemistry

Synthesis and evaluation of xylopyranoside derivatives as “decoy acceptors” of human β-1,4-galactosyltransferase 7

Bacterial symbionts induce a FUT2-dependent fucosylated niche on colonic epithelium via ERK and JNK signaling

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