In vivo chemotactic properties and spatial expression of PDGF in developing mesenteric microvascular networks

Zeller, Peter; Skalak, Thomas C.; Ponce, Aiyana; Price, Richard J.

doi:10.1152/ajpheart.2001.280.5.h2116

Cited by 17 publications

(17 citation statements)

References 29 publications

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“…During normal maturation, PDGF-β and its receptor PDGFR-β are expressed in a pattern that is consistent with a role for PDGF in mediating the microvascular development process [74]. During angiogenesis in the rat mesenteric window, capillary sprout ECs and pericytes migrate preferentially along resident elastic fibers.…”

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confidence: 54%

In vivo models of angiogenesis

2006

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confidence: 54%

In vivo models of angiogenesis

2006

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“…Reciprocal signaling of EC-induced SMC recruitment via PDGF has been examined in vivo (63,64), but not with events activated by external mechanical stimuli. More broadly, the findings of this study provide a specific example of how localized mechanical signals can be translated into biochemical cues (65) capable of signaling over physiologic relevant distances.…”

Section: Discussionmentioning

confidence: 99%

Cyclic tensile strain triggers a sequence of autocrine and paracrine signaling to regulate angiogenic sprouting in human vascular cells

Yung

Chae

Buehler

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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Mechanical signals regulate blood vessel development in vivo, and have been demonstrated to regulate signal transduction of endothelial cell (EC) and smooth muscle cell (SMC) phenotype in vitro. However, it is unclear how the complex process of angiogenesis, which involves multiple cell types and growth factors that act in a spatiotemporally regulated manner, is triggered by a mechanical input. Here, we describe a mechanism for modulating vascular cells during sequential stages of an in vitro model of early angiogenesis by applying cyclic tensile strain. Cyclic strain of human umbilical vein (HUV)ECs up-regulated the secretion of angiopoietin (Ang)-2 and PDGF-␤␤, and enhanced endothelial migration and sprout formation, whereas effects were eliminated with shRNA knockdown of endogenous Ang-2. Applying strain to colonies of HUVEC, cocultured on the same micropatterned substrate with nonstrained human aortic (HA)SMCs, led to a directed migration of the HASMC toward migrating HUVECs, with diminished recruitment when PDGF receptors were neutralized. These results demonstrate that a singular mechanical cue (cyclic tensile strain) can trigger a cascade of autocrine and paracrine signaling events between ECs and SMCs critical to the angiogenic process.angiogenesis ͉ angiopoietin-2 ͉ endothelial cells ͉ shRNA ͉ strain gradient

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“…[111][112][113] Methodical quantitative approaches have been applied to investigations of the soluble and mechanical factors governing the arterialization of existing microvascular networks. [114][115][116] Progress in developing a quantitative understanding of neovascularization continues to be limited by the available assays. Although complex therapies are under investigation, quantification of results of an intervention are often oversimplified.…”

Section: Quantitative Methodsmentioning

confidence: 99%

Therapeutic Neovascularization: Contributions from Bioengineering

et al. 2005

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A number of pathological entities and surgical interventions could benefit from therapeutic stimulation of new blood vessel formation. Although strategies designed for promoting neovascularization have shown promise in preclinical models, translation to human application has met with limited success when angiogenesis is used as the single therapeutic mechanism. While clinical protocols continue to be optimized, a number of exciting new approaches are being developed. Bioengineering has played an important role in the progress of many of these innovative new strategies. In this review, we present a general outline of therapeutic neovascularization, with an emphasis on investigations using engineering principles to address this vexing clinical problem. In addition, we identify some limitations and suggest areas for future research.

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In vivo chemotactic properties and spatial expression of PDGF in developing mesenteric microvascular networks

Cited by 17 publications

References 29 publications

In vivo models of angiogenesis

In vivo models of angiogenesis

Cyclic tensile strain triggers a sequence of autocrine and paracrine signaling to regulate angiogenic sprouting in human vascular cells

Therapeutic Neovascularization: Contributions from Bioengineering

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