In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p

Kent, Nicholas A.; Karabetsou, Nickoletta; Politis, Panagiotis K.; Mellor, Jane

doi:10.1101/gad.190301

Cited by 111 publications

(120 citation statements)

References 34 publications

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“…Consistent with transcriptional silencing of Pol II genes at the rDNA being a result of specialized silent chromatin, data presented here and elsewhere [7] suggest that Isw1 and Isw2 alter the function of silent chromatin at the rDNA. However, in contrast to the regulation of endogenous Ty1 elements located outside of the rDNA [20], our results suggest that ISW1 and ISW2 do not act redundantly in the silencing of Pol II-transcribed genes in the rDNA.…”

Section: Isw2 Regulates Transcriptional Silencing At the Rdnacontrasting

confidence: 87%

“…In contrast to the regulation of the Ty1his3AI-236 element in the rDNA, cells lacking either ISW1 or ISW2 individually do not exhibit defects in transcription of genomic Ty1 elements [7;20]. However, previous work has shown that cells lacking both ISW1 and ISW2 have higher levels of Ty1 transcripts than wild-type or single ISW1 or ISW2 deletion mutants, a result that suggests that Isw1 and Isw2 act redundantly to repress transcription of genomic Ty1 elements [20]. Consistent with transcriptional silencing of Pol II genes at the rDNA being a result of specialized silent chromatin, data presented here and elsewhere [7] suggest that Isw1 and Isw2 alter the function of silent chromatin at the rDNA.…”

Section: Isw2 Regulates Transcriptional Silencing At the Rdnamentioning

confidence: 99%

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Isw2 regulates gene silencing at the ribosomal DNA locus in Saccharomyces cerevisiae

Mueller

Bryk

2007

Biochemical and Biophysical Research Communications

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Three heterochromatin-like domains have been identified in S. cerevisiae that are refractory to transcription by Pol II, the silent mating-type loci, telomeres and the ribosomal DNA. Previous work has shown that chromatin remodelers can regulate silent chromatin. Here, we report the findings of an investigation into the role of ISW2 in transcriptional silencing at the rDNA. We show that the levels of retrotransposition and mRNA from a genetically marked Ty1 element located in the rDNA were increased significantly in isw2Δ cells, while transcript levels from Ty1 elements outside of the rDNA were not increased in cells lacking ISW2. Additionally, we show that Isw2 is not required for silencing at a telomere. Our findings demonstrate that Isw2 is required for transcriptional silencing at the rDNA and emphasize the differences in the regulation of transcriptional silencing at silent loci in S. cerevisiae.

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Section: Isw2 Regulates Transcriptional Silencing At the Rdnacontrasting

confidence: 87%

Section: Isw2 Regulates Transcriptional Silencing At the Rdnamentioning

confidence: 99%

Isw2 regulates gene silencing at the ribosomal DNA locus in Saccharomyces cerevisiae

Mueller

Bryk

2007

Biochemical and Biophysical Research Communications

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“…Deckert and K. Struhl, in prep. ), and recruitment of the ISW2 complex by the Ume6 repressor is important for transcriptional repression of target genes (Goldmark et al 2000;Fazzio et al 2001;Kent et al 2001). However, the connection between activatorand repressor-dependent recruitment to transcriptional output is not always straightforward.…”

Section: Constitutive and Regulated Recruitment Of Rsc In Response Tomentioning

confidence: 99%

“…SWI/SNF recruitment to the histone HTA1 promoter requires both Hir1 and Hir2 corepressors, although SWI/SNF contributes positively to transcription in this situation (Dimova et al 1999). The ISW2 complex is recruited to promoters by the Ume6 repressor, and it is important for repression of target genes (Goldmark et al 2000;Fazzio et al 2001;Kent et al 2001). These models are not mutually exclusive, and, indeed, histone acetylases and deacetylases have both promoter-specific and genome-wide activities (Kuo et al 2000;Reid et al 2000;Vogelauer et al 2000).…”

mentioning

confidence: 99%

Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex

Ng¹,

Robert²,

Young³

et al. 2002

Genes Dev.

255

292

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Genome-wide location analysis indicates that the yeast nucleosome-remodeling complex RSC has ∼700 physiological targets and that the Rsc1 and Rsc2 isoforms of the complex behave indistinguishably. RSC is associated with numerous tRNA promoters, suggesting that the complex is recruited by the RNA polymerase III transcription machinery. At RNA polymerase II promoters, RSC specifically targets several gene classes, including histones, small nucleolar RNAs, the nitrogen discrimination pathway, nonfermentative carbohydrate metabolism, and mitochondrial function. At the histone HTA1/HTB1 promoter, RSC recruitment requires the Hir1 and Hir2 corepressors, and it is associated with transcriptional inactivity. In contrast, RSC binds to promoters involved in carbohydrate metabolism in response to transcriptional activation, but prior to association of the Pol II machinery. Therefore, the RSC complex is generally recruited to Pol III promoters and it is specifically recruited to Pol II promoters by transcriptional activators and repressors.

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“…The Isw2-Itc1p complex was subsequently shown to be involved in the repression of other genes related to the starvation response, such as INO1 (Sugiyama & Nikawa, 2001) and PHO3 (Kent et al, 2001). The ISW2 gene encodes a yeast homologue of the Drosophila ISWI chromatin-remodelling ATPase (Tsukiyama et al, 1999;Trachtulcova et al, 2000), which is essential for development as well as for cell viability (Deuring et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Cell-type-dependent repression of yeast a-specific genes requires Itc1p, a subunit of the Isw2p–Itc1p chromatin remodelling complex

et al. 2003

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In Saccharomyces cerevisiae MATa haploid cells, the a-specific genes are expressed, whereas in the MATa haploid and MATa/a diploid cell types their transcription is repressed. It is shown in this report that Itc1p, a component of the ATP-dependent Isw2p-Itc1p chromatin remodelling complex, is required for the repression of a-specific genes. It has previously been reported that disruption of the ITC1 gene leads, in MATa cells, to an aberrant cell morphology resembling the polarized mating projection of cells responding to pheromone. The activation of the pheromone signalling pathway in itc1 mutants of both mating types was examined and found to be constitutively active in MATa itc1 but not in MATa itc1 cells. Furthermore, unlike the wild-type, MATa itc1 and MATa/a itc1/itc1 cells secrete a-factor and express significant levels of other a-specific genes. The results indicate that the inappropriate a-factor production in a MATa context, due to the derepression of the a-specific genes, produces an autocrine signalling loop that leads to the aberrant morphology displayed by MATa itc1 cells. It is suggested that the Isw2p-Itc1p complex contributes to maintain the repressive chromatin structure described for the asg operator present in the promoters of a-specific genes.

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In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p

Cited by 111 publications

References 34 publications

Isw2 regulates gene silencing at the ribosomal DNA locus in Saccharomyces cerevisiae

Isw2 regulates gene silencing at the ribosomal DNA locus in Saccharomyces cerevisiae

Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex

Cell-type-dependent repression of yeast a-specific genes requires Itc1p, a subunit of the Isw2p–Itc1p chromatin remodelling complex

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