In vivo comparative model of oxygen plasma and nanocomposite particles on PLGA membranes for guided bone regeneration processes to be applied in pre-prosthetic surgery: A pilot study

Castillo-Dalí, Gabriel; Castillo-Oyagüe, Raquel; Terriza, Antonia; Saffar, Jean‐Louis; Batista, A. C.; Barranco, Ángel; Cabezas-Talavero, Javier; Lynch, Christopher D.; Barouk, Brigitte; Llorens, Anni; Sloan, Alastair James; Cayón, Rocío V.; Gutiérrez-Pérez, José-Luís; Torrés-Lagares, Daniel

doi:10.1016/j.jdent.2014.04.015

Cited by 15 publications

(39 citation statements)

References 46 publications

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Section: Resultsmentioning

confidence: 99%

“…Figure 4 shows the macroscopic appearance of the PLGA membranes before and after surface treatment with oxygen plasma. Additional information about the PLGA membranes after and before the oxygen plasma treatments can be found elsewhere [1,2,[30][31][32][33]. Surface topography analysis also enables us to appreciate the considerable increase in surface roughness of the PLGA membrane after exposure to oxygen plasma, with an RMS roughness value greater than 300 nm, without affecting structural integrity.…”

Section: Resultsmentioning

confidence: 99%

“…The level of significance was set in advance at p ≤ 0.05 [6]. The Statview F 4.5 Macintosh software (Abacus Concepts, Berkeley, CA, USA) was used for the analysis [2]. All the statistical probes applied in this study adhere to the requirements for oral and dental research [29].…”

Section: Discussionmentioning

confidence: 99%

“…A randomly assigned membrane was used to cover each bone defect. The randomisation sequence was generated using specific software (Research Randomizer, V. 4.0, Urbaniak GC & Plous S, 2013) [2]. The PLGA fibres of the barriers were also randomly oriented.…”

Section: Surgical Proceduresmentioning

confidence: 99%

“…Tissue engineering enables the fabrication of membranes that mimic the structural properties of the original tissues, providing stable support to the extracellular matrix [2]. The application of polymeric biodegradable materials has become a common practice, however there is limited knowledge of the behaviour of the osteoblastic cells in them [3,4].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro and in Vivo Study of Poly(Lactic–co–Glycolic) (PLGA) Membranes Treated with Oxygen Plasma and Coated with Nanostructured Hydroxyapatite Ultrathin Films for Guided Bone Regeneration Processes

et al. 2017

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Abstract:The novelty of this study is the addition of an ultrathin layer of nanostructured hydroxyapatite (HA) on oxygen plasma modified poly(lactic-co-glycolic) (PLGA) membranes (PO 2 ) in order to evaluate the efficiency of this novel material in bone regeneration. Methods: Two groups of regenerative membranes were prepared: PLGA (control) and PLGA/PO 2 /HA (experimental). These membranes were subjected to cell cultures and then used to cover bone defects prepared on the skulls of eight experimental rabbits. Results: Cell morphology and adhesion of the osteoblasts to the membranes showed that the osteoblasts bound to PLGA were smaller and with a lower number of adhered cells than the osteoblasts bound to the PLGA/PO 2 /HA membrane (p < 0.05). The PLGA/PO 2 /HA membrane had a higher percentage of viable cells bound than the control membrane (p < 0.05). Both micro-CT and histological evaluation confirmed that PLGA/PO 2 /HA membranes enhance bone regeneration. A statistically significant difference in the percentage of osteoid area in relation to the total area between both groups was found. Conclusions: The incorporation of nanometric layers of nanostructured HA into PLGA membranes modified with PO 2 might be considered for the regeneration of bone defects. PLGA/PO 2 /HA membranes promote higher osteosynthetic activity, new bone formation, and mineralisation than the PLGA control group.

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