In Vivo Confocal Microscopy Study of Blebs after Filtering Surgery

Validation of the Glaucoma Filtration Surgical Mouse Model for Antifibrotic Drug Evaluation

“…These two MMCinduced effects in our mouse model parallel those observed previously in the rabbit (32) and the monkey (33) models. Furthermore, alterations in the in vivo bleb structure in our model were reminiscent of those observed previously in the clinic (24). Histologic features also mirrored those observed in the clinic with reduced matrix deposition in the MMC eyes compared with the control eyes.…”

“…unoperated eye (Figure 4B, i). These spaces may correspond with microcysts which were reported to be numerous in functioning human blebs (24). The subconjunctival connective tissue also appeared to be arranged loosely in the control day 2 bleb (Figure 4B, ii, iii).…”

Non-contact in vivo scanning laser microscopy of blebitis

“…2,3 The Rostock Cornea Module (RCM) has been successfully used to image microstructural changes in filtering blebs. 4,5 We report the RCM findings of blebitis before and 3 months after treatment.…”

Trypan blue staining of filtering bleb in eyes with operated trabeculectomy

“…They found that functional blebs have lower temperature than non-functional blebs and their morphological appearances are not affected by their temperature. In vivo evaluation techniques such as confocal scan (Messmer et al 2006, Labbe et al, 2005 and ultrasound biomicroscopy (Yamamoto et al 1995) have also been used to evaluate the morphology of these blebs. However, no clinical studies have been performed in literature to evaluate bleb function intra-operatively during phacoemulsification.…”

“…Many authors (Pitch et al 1998, Clarke et al 2003 have investigated morphological criteria of these blebs to correlate clinical and functional aspects. Although some authors have described the evaluation of filtering bleb by thermography (Kawasaki et al 2009) and in vivo confocal microscopy (Messmer et al 2006, Labbe et al 2005, there is still a lack of clinical tests that can objectively delineate the functional status of the bleb.…”