In vivo confocal Raman spectroscopy for intrinsic aging and photoaging assessment

Caetano, Livia de Vasconcelos Nasser; Mendes, Thiago de Oliveira; Bagatin, Ediléia; Miot, Hélio Amante; Soares, Juliana L M; Enokihara, Mílvia Maria Simões e Silva; Martin, Aírton Abrahão

doi:10.1016/j.jdermsci.2017.07.011

Cited by 15 publications

(7 citation statements)

References 37 publications

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“…Confocal Raman microscopy (CRM) is a powerful non‐invasive tool to measure depth profiles of molecular components in the SC , in which the skin components are measured depth‐dependently in vivo / ex vivo at high spatial resolution. An area of interest using CRM in dermatology is the analysis of the penetration into the skin , the skin barrier function provided by the SC , and collagen of the dermis . Our group recently introduced methods to determine the molecular properties of skin barrier function‐related parameters, such as water bonding states , ICL ordering and secondary and tertiary structures of keratin in the SC in vivo.…”

Section: Introductionmentioning

confidence: 99%

Human skin in vivo has a higher skin barrier function than porcine skin ex vivo—comprehensive Raman microscopic study of the stratum corneum

Choe¹,

Schleusener

Lademann

et al. 2018

Journal of Biophotonics

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Porcine skin is widely used as a human skin model in dermatology. For both, porcine stratum corneum (SC) ex vivo and human SC in vivo, the hydrogen bonding states of water, the secondary and tertiary structures of keratin, the natural moisturizing factor (NMF) concentrations and the intercellular lipids' (ICL) lateral organization are investigated depth-dependently using confocal Raman microscopy. The SC depth profiles show that porcine SC ex vivo is characterized by lower hydrogen bonding states of water (10%-30% SC depth), lower NMF concentration in the whole SC, more β-sheet form of keratin (10%-90% SC depth), more folded tertiary keratin structures (30%-70% SC depth) and higher hexagonal lateral packing order of ICL (10%-50% SC depth) compared to human SC in vivo. The results clearly show a higher value of skin barrier function of human SC in vivo than of porcine SC ex vivo. Thus, the human SC in vivo is less permeable for lipophilic and hydrophilic substances than porcine SC ex vivo. Considering the porcine SC as an ex vivo model of human SC in vivo, these findings should be taken into consideration.

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Section: Introductionmentioning

confidence: 99%

Human skin in vivo has a higher skin barrier function than porcine skin ex vivo—comprehensive Raman microscopic study of the stratum corneum

Choe¹,

Schleusener

Lademann

et al. 2018

Journal of Biophotonics

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“…On the papillary dermis, we also observed that chronological aging was associated with a decrease of the 938/922 ratio. This result is in agreement with a recent study performed in vivo on the papillary dermis, where de Vasconcelos Nasser Caetano et al attributed the reduction of the 855 cm −1 and 938 cm −1 band intensities to collagen alterations during intrinsic aging. Zhang et al showed that the 938 cm −1 band provides a measurement of the collagen highly bonded with water .…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, identification of Raman spectral bands for collagen has already been performed by several teams . This is a very suitable technique for determining molecular and structural modifications occurring during biological process such as skin aging . Gniadecka et al studied the water and protein structure in photoaged and chronically aged skin.…”

Section: Introductionmentioning

confidence: 99%

“…[16][17][18] This is a very suitable technique for determining molecular and structural modifications occurring during biological process such as skin aging. 10,14,15,19,20 Gniadecka et al studied the water and protein structure in photo aged and chronically aged skin. They observed a decrease in amide III band intensity in the photo aged skin showing a change in the protein conformational structure.…”

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confidence: 99%

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Raman characterization of human skin aging

Villaret

Ipinazar

Satar

et al. 2018

Skin Research and Technology

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Background Skin aging is a complex biological process mixing intrinsic and extrinsic factors, such as sun exposure. At the molecular level, skin aging affects in particular the extracellular matrix proteins. Materials and Methods Using Raman imaging, which is a nondestructive approach appropriate for studying biological samples, we analyzed how aging modifies the matrix proteins of the papillary and reticular dermis. Biopsies from the buttock and dorsal forearm of volunteers younger than 30 and older than 60 were analyzed in order to identify chronological and photoaging processes. Analyses were performed on skin section, and Raman spectra were acquired separately on the different dermal layers. Results We observed differences in dermal matrix structure and hydration state with skin aging. Chronological aging alters in particular the collagen of the papillary dermis, while photoaging causes a decrease in collagen stability by altering proline and hydroxyproline residues in the reticular dermis. Moreover, chronological aging alters glycosaminoglycan content in both dermal compartments. Conclusion Alterations of the papillary and reticular dermal matrix structures during photo‐ and chronological aging were clearly depicted by Raman spectroscopy.

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“…Peak assignment (Movasaghi et al 2007) Bai et al intrinsic aging and photoaging in vivo in 15 subjects between 28 and 82 years of age, divided into three different groups (de Vasconcelos Nasser Caetano et al 2017). The authors pointed out the prolinehydroxyproline region (intensities of peaks at 855 cm −1 and 938 cm −1 ) as suitable for the evaluation of intrinsic skin aging.…”

Section: Spectral Regionmentioning

confidence: 99%

Raman fingerprints as promising markers of cellular senescence and aging

2019

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Due to our aging population, understanding of the underlying molecular mechanisms constantly gains more and more importance. Senescent cells, defined by being irreversibly growth arrested and associated with a specific gene expression and secretory pattern, accumulate with age and thus contribute to several age-related diseases. However, their specific detection, especially in vivo, is still a major challenge. Raman microspectroscopy is able to record biochemical fingerprints of cells and tissues, allowing a distinction between different cellular states, or between healthy and cancer tissue. Similarly, Raman microspectroscopy was already successfully used to distinguish senescent from non-senescent cells, as well as to investigate other molecular changes that occur at cell and tissue level during aging. This review is intended to give an overview about various applications of Raman microspectroscopy to study aging, especially in the context of detecting senescent cells.

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In vivo confocal Raman spectroscopy for intrinsic aging and photoaging assessment

Cited by 15 publications

References 37 publications

Human skin in vivo has a higher skin barrier function than porcine skin ex vivo—comprehensive Raman microscopic study of the stratum corneum

Human skin in vivo has a higher skin barrier function than porcine skin ex vivo—comprehensive Raman microscopic study of the stratum corneum

Raman characterization of human skin aging

Raman fingerprints as promising markers of cellular senescence and aging

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