In-vivo confocal real-time mini-microscopy in animal models of human inflammatory and neoplastic diseases

Goetz, Martin; Fottner, Christian; Schirrmacher, Esther; Delaney, Peter; Gregor, Sebastian; Schneider, C.; Strand, Dennis; Kanzler, Stephan; Memadathil, B.; Weyand, E; Holtmann, Martin; Schirrmacher, Ralf; Weber, M M; Anlauf, Martin; Klöppel, G.; Vieth, Michael; Galle, Peter R.; Bartenstein, Peter; Neurath, Markus F.; Kießlich, Ralf

doi:10.1055/s-2007-966262

Cited by 71 publications

(74 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The imaging plane can be stepped down by increments of 4 mm, to 250 mm below the surface, and resolutions vary depending on the imaging acquisition speed: 1024 Â 1024 pixels (0.8 frames/s) or 1024 Â 512 pixels (1.6 frames/s). 16,17 Post-treatment of images was performed with Adobe Photoshop (Adobe Systems, San Jose, CA, USA). The confocal probe was introduced through either transthoracic or transdiaphragmatic routes and secured on the pleural surface.…”

Section: Materials and Methods Experimental Animal Modelsmentioning

confidence: 99%

In vivo intravital endoscopic confocal fluorescence microscopy of normal and acutely injured rat lungs

Chagnon

Fournier

Charette

et al. 2010

Laboratory Investigation

View full text Add to dashboard Cite

We present a new lung imaging technique based on endoscopic confocal fluorescence microscopy (ECFM), which is a new method that is able to provide cellular and structural assessment of living tissue using a small confocal probe in direct contact with the visceral pleura. To observe distal airspace structure and cellular condition in normal and injured lungs (hyperoxic and bleomycin challenged), we used fluorescent-specific marker contrast and ECFM. Alveolar space ECFM with spectral analyses were performed at 488-nm excitation using FITC-labeled markers or naturally fluorescent dyes. The normal lung was compared with the sick lung, where our in vivo imaging experiments correlated well with results obtained with corresponding ex vivo conventional assays. Four main elements pertaining to the acute lung injury/ acute respiratory distress syndrome (ALI/ARDS) pathophysiology and established early key events were specifically studied: alveolar epithelial membrane phenotype, lung cell apoptosis, neutrophil recruitment, and edema. ECFM allowed visualization of (i) fine-tuned ultrastructural lectin (RCA-1) and sialoglycoprotein (RTI40) epithelial cell membrane expression, (ii) YO-PRO-1-related DNA linking of lung cell apoptosis, (iii) PKH2 green fluorescent cell linker-labeled neutrophil tracking in lung microcirculatory network and airspaces, (iv) FITC-dextran plasma contrast and extravasation with edema formation. ECFM provides reliable results to corresponding ex vivo fluorescent methods. ECFM, using the minimally invasive Five-1s optical instrument and specific fluorescent markers, is able to provide real-time potentially useful imaging of live unfixed normal and injured lung tissue with promising developments for improving bedside diagnostic and decision-making therapeutic strategy in patients with ALI.

show abstract

Section: Materials and Methods Experimental Animal Modelsmentioning

confidence: 99%

In vivo intravital endoscopic confocal fluorescence microscopy of normal and acutely injured rat lungs

Chagnon

Fournier

Charette

et al. 2010

Laboratory Investigation

View full text Add to dashboard Cite

show abstract

“…Although the excitation and emission wavelengths do not peek within the above given range, the broad spectrum of cresyl violet still resulted in indirect nuclear visualization by cytoplasmic enrichment of the dye [15]. In animal studies with lower regulatory requirements a multitude of staining protocols have been evaluated in vivo [16], and even antibodies have been fluorescently labeled.…”

Section: In Vivo Imaging and Staining Protocolsmentioning

confidence: 99%

“…The confocal scanner that was later integrated into eCLE endoscopes has been available for laboratory use for years (FIVE1, Optiscan) [85][86][87], and also the probe based system can be used in the laboratory. Animal studies do not face the limitations and safety constraints of human trials so that many of the contrast agents used for bench top laser scanning microscopy can be used in vivo [16]. Initial studies have evaluated intravenous fluorescein, that was later rapidly transferred into human applications, but also used intravenous acriflavine.…”

Section: Functional and Molecular Imaging With Endomicroscopymentioning

confidence: 99%

“…Leakage of labeled Lycopersicon esculentum-Lectin that was used to label vessel walls in vivo was found helpful to identify metastatic foci of few cells in the liver [16]. The combination of different dyes allowed comprehensive imaging of tissue and vessel function against a background of tissue morphologic staining [89,92], although multiwavelength imaging and overlay of different excitation and emission lines is not (yet) possible.…”

Section: Functional and Molecular Imaging With Endomicroscopymentioning

confidence: 99%

“…Such imaging of receptors or ligands overexpressed in tumors is integrated into therapy protocols of neuroendocrine tumors, for example, by scintigraphy. With CLE, imaging of somatostatin receptor has been achieved in murine models of neuroendocrine tumors [16,105]. This concept of in vivo tumor imaging has been supplemented by using not just diagnostic, but therapeutic antibodies.…”

Section: Molecular Imagingmentioning

confidence: 99%

See 2 more Smart Citations

Confocal Laser Endomicroscopy: Applications in Clinical and Translational Science—A Comprehensive Review

Goetz

2012

ISRN Pathology

View full text Add to dashboard Cite

Confocal laser endomicroscopy (CLE) is a novel tool in the endoscopist's armamentarium. It allows on-site histological information. The ability of gastroenterologists to interpret such microscopic information has been demonstrated in multiple studies from the upper and lower gastrointestinal tract. Recently, the field of application has expanded to provide hepatobiliary and intra-abdominal CLE imaging. CLE allows "smart," targeted biopsies and is able to guide endoscopic interventions. But CLE is also translational in its approach and permits functional imaging that significantly impacts on our understanding of gastrointestinal diseases. Molecular imaging with CLE allows detection and characterization of lesions and may even be used for prediction of response to targeted therapy. This paper provides a comprehensive review over current applications of CLE in clinical applications and translational science.

show abstract

Endoscopic Confocal and Molecular Imaging in Hepatobiliary Disease

Hoetker¹,

Goetz²

2017

Endoscopy in Liver Disease

View full text Add to dashboard Cite

In-vivo confocal real-time mini-microscopy in animal models of human inflammatory and neoplastic diseases

Cited by 71 publications

References 18 publications

In vivo intravital endoscopic confocal fluorescence microscopy of normal and acutely injured rat lungs

In vivo intravital endoscopic confocal fluorescence microscopy of normal and acutely injured rat lungs

Confocal Laser Endomicroscopy: Applications in Clinical and Translational Science—A Comprehensive Review

Endoscopic Confocal and Molecular Imaging in Hepatobiliary Disease

Contact Info

Product

Resources

About