In vivo cross-sectional imaging of the phonating larynx using long-range Doppler optical coherence tomography

Coughlan, Carolyn A.; Chou, Li-Dek; Jing, Joseph C.; Chen, Jason J.; Rangarajan, Swathi; Chang, Theodore H.; Sharma, Giriraj K.; Cho, Kyoung Rai; Lee, Dong‐Hoon; Goddard, Julie; Chen, Zhongping; Wong, Brian J. F.

doi:10.1038/srep22792

Cited by 31 publications

(29 citation statements)

References 44 publications

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“…Based upon system configuration, various low coherent near‐infrared light sources are used, including super luminescent diodes (SLD) and swept vertical cavity surface emitting lasers (VCSELs) . Various OCT probes and hand piece designs—including microscopes, rigid endoscopes, and flexible endoscopes—allow for forward viewing , side viewing , or rotary viewing . Using a TD system with a forward facing probe, Sergeev et al first demonstrated the feasibility of using endoscopic OCT to image the mucosa of various organs, including the larynx .…”

Section: Introductionmentioning

confidence: 99%

“…There are several OCT system configurations: time domain (TD), and Fourier domain techniques including swept source (SS) and spectral domain (SD) [8][9][10]. Based upon system configuration, various low coherent near-infrared light sources are used, including super luminescent diodes (SLD) [8] and swept vertical cavity surface emitting lasers (VCSELs) [11]. Various OCT probes and hand piece designs-including microscopes, rigid endoscopes, and flexible endoscopesallow for forward viewing [8,[12][13][14][15][16][17][18][19][20][21][22][23][24][25], side viewing [11,[26][27][28][29][30][31][32], or rotary viewing [33,34].…”

Section: Introductionmentioning

confidence: 99%

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Computational analysis of six optical coherence tomography systems for vocal fold imaging: A comparison study

et al. 2019

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Objectives There have been many advancements in laryngeal imaging using optical coherence tomography (OCT), with varying system design and probes for use in research, office, and operating room settings. We evaluated the performance of six distinct OCT systems in imaging porcine vocal folds (cords) using computational image processing and segmentation. Methods Porcine vocal folds were scanned using six OCT systems. Imaging system and probe performance were quantitatively assessed for signal penetration, layer differentiation, and epithelium (EP) measurement. Fitted exponential decay curves with corresponding α constant and intensity thresholding segmentation were utilized to quantify the aforementioned parameters. Results The smallest average α constant and deepest signal penetration was of the SS‐OCT 1700 nm 90 kHz microscope system (α = −1.74), followed by the SS‐OCT 1310 nm 200 kHz VCSEL microscope system (α = −1.99), and SS‐OCT 1310 nm 50 kHz rigid forward viewing endoscope system (α = −2.23). The EP was not readily visualized for three out of six systems, but was detected using automated segmentation. Average EP thickness (mean ± SD) was calculated as 55.79 ± 31.86 μm which agrees favorably with previous literature. Conclusion Comparisons of OCT systems are challenging, as they encompass different probe design, optical path, and lasers, depending on application. Practical evaluation of different systems using computer based quantitative image processing and segmentation revealed basic, constructive information, such as EP measurements. To further validate the comparisons of system performance with clinical usability, in vivo human laryngeal imaging will be conducted. Further development of automated image processing and segmentation can be useful in rapid analysis of information. Lasers Surg. Med. 51:412–422, 2019. © 2019 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Computational analysis of six optical coherence tomography systems for vocal fold imaging: A comparison study

et al. 2019

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“…Optical coherence tomography (OCT) and high-frequency (>30 kHz) ultrasound provide high-resolution, cross-sectional imaging of tissues and have been used to evaluate naïve, pathologic and surgically manipulated vocal fold mucosae in preclinical models and human patients (Burns et al, 2011, 2009; Coughlan et al, 2016; Huang et al, 2007; Walsh et al, 2008; Wong et al, 2005). Imaging data are available in real time; however, with the exception of long-range OCT (Coughlan et al, 2016; Vokes et al, 2008), these techniques require endolaryngeal placement of an imaging probe used in contact or near-contact mode, have limited depth penetration and do not provide full anatomic context for the region of interest. Magnetic resonance imaging (MRI) is an alternative technology that allows high-resolution, high-contrast imaging of whole tissues.…”

Section: Introductionmentioning

confidence: 99%

High- and ultrahigh-field magnetic resonance imaging of naïve, injured and scarred vocal fold mucosae in rats

Kishimoto

Young

et al. 2016

Disease Models &Amp; Mechanisms

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Subepithelial changes to the vocal fold mucosa, such as fibrosis, are difficult to identify using visual assessment of the tissue surface. Moreover, without suspicion of neoplasm, mucosal biopsy is not a viable clinical option, as it carries its own risk of iatrogenic injury and scar formation. Given these challenges, we assessed the ability of high- (4.7 T) and ultrahigh-field (9.4 T) magnetic resonance imaging to resolve key vocal fold subepithelial tissue structures in the rat, an important and widely used preclinical model in vocal fold biology. We conducted serial in vivo and ex vivo imaging, evaluated an array of acquisition sequences and contrast agents, and successfully resolved key anatomic features of naïve, acutely injured, and chronically scarred vocal fold mucosae on the ex vivo scans. Naïve lamina propria was hyperintense on T1-weighted imaging with gadobenate dimeglumine contrast enhancement, whereas chronic scar was characterized by reduced lamina propria T1 signal intensity and mucosal volume. Acutely injured mucosa was hypointense on T2-weighted imaging; lesion volume steadily increased, peaked at 5 days post-injury, and then decreased – consistent with the physiology of acute, followed by subacute, hemorrhage and associated changes in the magnetic state of hemoglobin and its degradation products. Intravenous administration of superparamagnetic iron oxide conferred no T2 contrast enhancement during the acute injury period. These findings confirm that magnetic resonance imaging can resolve anatomic substructures within naïve vocal fold mucosa, qualitative and quantitative features of acute injury, and the presence of chronic scar.

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“…*Correspondence to: Mette Pedersen, The Medical Center, Ear Nose Throat Unit, Oestergade 18, DK-1100 Copenhagen, Denmark, Email: m.f.pedersen@dadlnet.dk O ptical coherence tomography (OCT) is an imaging technology that provides cross-sectional images without biopsy of subsurface tissue structure at approximately 10-mm resolution to a depth of 1.5 mm using backscattered light. OCT has shown promise in imaging normal vocal folds as well as various laryngeal disorders (1). The use of OCT to image the larynx during diagnosis and treatment of a vast array of laryngeal disorders continues to develop along with innovative surgical techniques.…”

Section: In Contextmentioning

confidence: 99%

Optical coherence tomography in the laryngeal arytenoid mucosa for documentation of pharmacological treatments and genetic aspects: a protocol

Pedersen¹,

Agersted²,

Akram³

et al. 2016

Advances in Cellular and Molecular Otolaryngology

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Background: Optical coherence tomography (OCT) is an imaging technology that provides cross-sectional images of subsurface tissue structures in the larynx without biopsy. Focus has until now been on the vocal folds. Since many kinds of mucosal disorders can be defined in the arytenoid regions behind the vocal folds, and not on the vocal folds, we focus on this area in this protocol. Objective: A superficial oedema, grading 1Á5 of the arytenoid regions, can be made with high-speed films of 4,000 pictures/ sec. A supplementary larynx set up with a clinical probe for OCT with cross-sectional measures can be made with a description of the levels of oedema and other parameters. Design: Adult patients with subjective complaints of hoarseness or a problem in the larynx related to voice and swallowing for more than 6 weeks participated in a randomised-controlled study. Perspective and conclusion: With this new OCT probe for the larynx arytenoid region, compared with high-speed films, we will be able to define pathological areas in the arytenoid mucosa as a future documentation of pharmacological treatment and genetics-related lifestyle advice.

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In vivo cross-sectional imaging of the phonating larynx using long-range Doppler optical coherence tomography

Cited by 31 publications

References 44 publications

Computational analysis of six optical coherence tomography systems for vocal fold imaging: A comparison study

Computational analysis of six optical coherence tomography systems for vocal fold imaging: A comparison study

High- and ultrahigh-field magnetic resonance imaging of naïve, injured and scarred vocal fold mucosae in rats

Optical coherence tomography in the laryngeal arytenoid mucosa for documentation of pharmacological treatments and genetic aspects: a protocol

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