In vivo cytogenetic studies on blends of aspartame and acesulfame-K

Mukhopadhyay, Mahuya; Mukherjee, Anita; Chakrabarti, J.

doi:10.1016/s0278-6915(99)00115-5

Cited by 41 publications

(23 citation statements)

References 3 publications

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“…Several previous studies have revealed that the use of artificial sweeteners may entail some hazards to the users. 40 In the present study, the observed high significant decrease in serum blood glucose concentration of saccharin-treated rats when compared to the control level was in good agreement with that reported 41 in animals, where they found that chronic consumption of large amounts of saccharin reduced blood glucose concentration. On the other hand, Abdallah 23 noticed a significant drop in blood sugar in rats administered saccharin, with an increase in liver glycogen level.…”

Section: Discussionsupporting

confidence: 93%

Effect of saccharin on albino rats’ blood indices and the therapeutic action of vitamins C and E

Abdelaziz

Ashour

2010

Hum Exp Toxicol

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The present work aimed to study some blood indices of rats as affected by saccharin and the therapeutic action of vitamins C and E. The used adult female Rattus norvegicus albino rats in the present study were weighing 100—120 g. Administration of saccharin at a dose of 35 mg kg—1 body weight (b.wt.) day—1 for 35 days significantly decreased serum glucose, triglycerides, cholesterol, total protein and albumin values. These decrements were by 20.16%, 22.76%, 44.92%, 20.16% and 40.44%, respectively, compared to control level (p value < 0.01). But it increased levels of kidney function indices. The effect of saccharin was more pronounced on creatinine. Activities of Alanine aminotranferease (ALT), aspartate aminotransferase (AST) and Alkaline phosphatase (ALP) increased significantly following saccharin treatment to rats. Concerning hematoligical parameters, the more obvious changes were observed in the increment of white blood cell (WBC), mean corpuscular volume (MCV) and platelets (PLT) and the decrease in hematocrit, hemoglobin (Hb) and red blood cells (RBCs) count in response to the administration of saccharin. In general, vitamin C or E (150 mg kg—1 b.wt. day—1 for 35 days) was able to reduce the effects of saccharin intake. Both vitamins, however, generally have beneficial effects in reducing the changes in the studied parameters.

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Section: Discussionsupporting

confidence: 93%

Effect of saccharin on albino rats’ blood indices and the therapeutic action of vitamins C and E

Abdelaziz

Ashour

2010

Hum Exp Toxicol

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“…Chromosomal aberration assays in bone marrow of Swiss albino mice that had been fed blends of aspartame and acesulfame-K did not show any genotoxic activity (Mukhopadhyay et al, 2000). Contradictorily, acesulfame K at dose below the recommended no-toxic-effects amounts showed positive clastogenic potentials in a similar study (Mukherjee and Chakrabarti, 1997).…”

Section: In Vivo Assaysmentioning

confidence: 83%

Toxicity of food colours and additives: A review

Thomas¹,

Adegoke²

2015

Afr. J. Pharm. Pharmacol.

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Majority of consumer goods are required to be presented with good aesthetics in order to improve acceptability in terms of colours and in some instances taste. When related to food, beverages and drug products, additives are usually added to mask un-inviting colours, obscure offensive odours and increase taste. Food additives therefore include colourants, sweeteners, preservatives and anti-caking agents. Admissible daily intake limits are often recommended for these additives. Being food products, the amount consumed over time may be subject to individual preferences and thus negating the desire to regulate and control the amount consumed cumulatively. There have been several concerns about the safety of food additives and several batteries of tests, and reports are available in literature. This review attempted to give an update on reports that have surfaced in literature over recent past on the use and safety of food colours and other additives. Some safety concerns have been related to three determinations; cytotoxicity, genotoxicity and induction or potential of inducing mutagenicity. In order to accomplish these targeted evaluations, several tests have been prescribed by International conference on harmonization (ICH), organization for economic co-operation and development (OECD) and European food safety authority (EFSA). It is observed that no single test can give a full proof of safety of these food colours and additives, hence minimal tests are recommended to be carried out in order to guarantee safety of these products. Survey of literature, revealed that once some approved additives or colours become a subject of safety concerns, comprehensive evaluations are carried out by researchers and these have often led to the de-classification of some hitherto reported agents as being non-genotoxic or non-carcinogenic. The declassifications of some food colors and additives as human carcinogens are regularly done following the comprehensive evaluation of results of mutagenicity and genotoxicity tests in vitro and some in vivo tests in mammalian tissues and whole animals. However, such declassifications are often done with caution and the implication is that regular and more comprehensive tests must be carried out. In addition, the requirements of testing for chronic exposures to this and other agents must be emphasized to prevent occurrence of subtle yet terrible side effects resulting from consuming sub-toxic doses of the additives over time.

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“…And it was reported that aspartame did not induce DNA damage in rat hepatocytes [5]. Mukhopadhyay et al [6] reported in vivo coexposure of aspartame and acesulfame potassium was negative for the induction of chromosome aberrations in male Swiss mice bone marrow cells. Although, Ishii [7] reported that aspartame does not cause brain cancer in rats.…”

Section: Introductionmentioning

confidence: 99%

In VivoCytogenetic Studies on Aspartame

AlSuhaibani

2010

Comparative and Functional Genomics

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Aspartame (a-Laspartyl-L-phenylalanine 1-methylester) is a dipeptide low-calorie artificial sweetener that is widely used as a nonnutritive sweetener in foods and drinks. The safety of aspartame and its metabolic breakdown products (phenylalanine, aspartic acid and methanol) was investigated in vivo using chromosomal aberration (CA) test and sister chromatid exchange (SCE) test in the bone marrow cells of mice. Swiss Albino male mice were exposed to aspartame (3.5, 35, 350 mg/kg body weight). Bone marrow cells isolated from femora were analyzed for chromosome aberrations and sister chromatid exchanges. Treatment with aspartame induced dose dependently chromosome aberrations at all concentrations while it did not induce sister chromatid exchanges. On the other hand, aspartame did not decrease the mitotic index (MI). However, statistical analysis of the results show that aspartame is not significantly genotoxic at low concentration.

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In vivo cytogenetic studies on blends of aspartame and acesulfame-K

Cited by 41 publications

References 3 publications

Effect of saccharin on albino rats’ blood indices and the therapeutic action of vitamins C and E

Effect of saccharin on albino rats’ blood indices and the therapeutic action of vitamins C and E

Toxicity of food colours and additives: A review

In VivoCytogenetic Studies on Aspartame

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