In vivo degradation of low temperature calcium and magnesium phosphate ceramics in a heterotopic model

Klammert, Uwe; Ignatius, Anita; Wolfram, Uwe; Reuther, T.; Gbureck, Uwe

doi:10.1016/j.actbio.2011.05.022

Cited by 122 publications

(98 citation statements)

References 25 publications

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“…25 There are however some issues with the long-term fate of large volumes of implanted brushite, as it has been shown to transform into more stable phases like OCP and HAp. 26,27 This effect seems to be site-specific and is likely related to the in vivo fluid exchange in the sample location. 28,29 Such behavior has implications for the choice of brushite to fill large bone lesions.…”

Section: Introductionmentioning

confidence: 99%

Controlled mineralisation and recrystallisation of brushite within alginate hydrogels

Bjørnøy¹,

Bassett²,

Ucar³

et al. 2016

Biomed. Mater.

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Abstract. Due to high solubility and resorption behaviour under physiological conditions, brushite (CaHPO 4 ·2H 2 O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as component of calcium phosphate cements. The use of brushite in combination with hydrogels opens possibilities for new cell based tissue engineering applications of this promising material. However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over mineral phase, content and distribution within the hydrogel matrix. The main focus of this study was to address these shortcomings by determining precise fabrication parameters needed to prepare composites with controlled composition and properties. Composite alginate microbeads were prepared using a counter-diffusion technique which allows for simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase. Reliable nucleation of a desired mineral phase within the alginate network proved more challenging than simple aqueous precipitation. This was largely due to ion transport within the hydrogel producing concentration gradients that modified levels of supersaturation and favoured the nucleation of other phases such as hydroxyapatite and octacalcium phosphate which would otherwise not form. To overcome this, incorporation of brushite seed crystals resulted in good control over the mineral phase and by adjusting the amount of seeds and precursor concentration, the amount of mineral could be tuned. The material has been characterized with a range of physical techniques, including scanning electron microscopy, powder X-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy and thermogravimetric analysis, in order to assess mineral morphology, phase and amount within the organic matrix. The mineral content of the composite material converted from brushite into hydroxyapatite when submerged in simulated body fluid, indicating possible bioactivity. Additionally, initial cell culture studies revealed that both the material and the synthesis procedure is compatible with cells relevant to bone tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Controlled mineralisation and recrystallisation of brushite within alginate hydrogels

Bjørnøy¹,

Bassett²,

Ucar³

et al. 2016

Biomed. Mater.

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show abstract

“…In the natural processes, CP can supply the phosphate group to adenosine diphosphate (ADP) and restore the normal levels of adenosine triphosphate (ATP) under the catalysis of creatine kinase [17]. Therefore, CP biomolecules are excellent biocompatible organic phosphorus source for the synthesis of phosphate nanostructured biomaterials, which can avoid the fast nucleation and disordered growth of the product because the phosphorus source exists in the form of phosphate groups in CP biomolecules instead of free PO 4 3-ions in aqueous solution. Moreover, the hydrolysis of CP biomolecules generally requires certain heating conditions for the formation of free phosphate ions in aqueous solution, so the hydrolysis parameters can be used to control the hydrolysis rate of CP biomolecules, which determine the morphology, size, and structure of the product [16,18].…”

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confidence: 99%

“…Moreover, magnesium is one of the key ingredients of bone mineral which can influence bone mineral formation, crystallization processes and metabolism [1][2][3]. Therefore, magnesium containing biomaterials are excellent candidates for biomedical applications owing to their outstanding biocompatibility and biodegradability [4]. For example, magnesium-doped calcium phosphates can improve their stability, and significantly enhance osteoblast attachment and growth [5,6].…”

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confidence: 99%

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Magnesium phosphate pentahydrate nanosheets: Microwave-hydrothermal rapid synthesis using creatine phosphate as an organic phosphorus source and application in protein adsorption

Zhu

et al. 2016

Journal of Colloid and Interface Science

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“…3,4 Over the past few years, Mg-containing bioactive materials have received increased attention for bone tissue repair, and several magnesium-based biomaterials were reported, such as Mg and its alloys, magnesium-containing bioactive glasses and coatings, and Mg-substituted calcium phosphate bioceramics/biocements, and so on. [5][6][7][8] These studies have shown that Mg as an important element in the bioactive materials can have some special effects on the material properties.…”

Section: Introductionmentioning

confidence: 99%

Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration

Xia¹,

Zhou²,

Wang³

et al. 2016

IJN

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In this study, bioactive scaffold of nano magnesium phosphate (nMP)/wheat protein (WP) composite (MWC) was fabricated. The results revealed that the MWC scaffolds had interconnected not only macropores (sized 400–600 μm) but also micropores (sized 10–20 μm) on the walls of macropores. The MWC scaffolds containing 40 w% nMP had an appropriate degradability in phosphate-buffered saline and produced a weak alkaline microenvironment. In cell culture experiments, the results revealed that the MWC scaffolds significantly promoted the MC3T3-E1 cell proliferation, differentiation, and growth into the scaffolds. The results of synchrotron radiation microcomputed tomography and analysis of the histological sections of the in vivo implantation revealed that the MWC scaffolds evidently improved the new bone formation and bone defects repair as compared with WP scaffolds. Moreover, it was found that newly formed bone tissue continued to increase with the gradual reduction of materials residual in the MWC scaffolds. Furthermore, the immunohistochemical analysis further offered the evidence of the stimulatory effects of MWC scaffolds on osteogenic-related cell differentiation and new bone regeneration. The results indicated that MWC scaffolds with good biocompability and degradability could promote osteogenesis in vivo, which would have potential for bone tissue repair.

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In vivo degradation of low temperature calcium and magnesium phosphate ceramics in a heterotopic model

Cited by 122 publications

References 25 publications

Controlled mineralisation and recrystallisation of brushite within alginate hydrogels

Controlled mineralisation and recrystallisation of brushite within alginate hydrogels

Magnesium phosphate pentahydrate nanosheets: Microwave-hydrothermal rapid synthesis using creatine phosphate as an organic phosphorus source and application in protein adsorption

Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration

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