In vivo delivery of siRNA to the brain by carbosilane dendrimer

Fluorescein labelled cationic carbosilane dendritic systems for biological studies

“…5). Previously, an analogous behaviour of these dendrimers was observed in primary normal human astrocytes (NHA) [56].…”

“…As synthetic strategy we have chosen the thiol-ene addition that also facilitates the access to heterofunctionalized systems [55]. Moreover, we have demonstrated that a second generation of cationic dendrimer synthesized and labelled with a fluorescein moiety form a stable dendriplex with siRNA Nef and crosses the BBB in a BALB/c mouse model, without histological and morphological changes in different brain regions [56].…”

Anticancer siRNA cocktails as a novel tool to treat cancer cells. Part (B). Efficiency of pharmacological action

“…The mechanisms of dendrimers' action differ in their nature: carbosilane dendrimers can induce cell apoptosis Weber et al, 2008;Wrobel et al, 2014;Serramía et al, 2015), PAMAM dendrimers -apoptosis (Mukherjee et al, 2010a,b, b;Mukherjee and Byrne, 2013) and autophagy Wang et al, 2014), while phosphorus dendrimers -necrosis (Lazniewska et al, 2013). Thus, our data suggest that inducing several mechanisms of programmed cell death is more effective in cancer treatment.…”

Nanoparticles for Brain-Specific Drug and Genetic Material delivery, Imaging and Diagnosis

“…Poly-lysine dendrimers (G3 and G5) were found in the periventricular zone and in the cytoplasm of neurons and glial cells in the forebrain and hippocampus after intraventricular administration to rats [64]. More recently, it has been also reported that a second-generation carbosilane dendrimer transported siRNA efficiently into the brain, crossing the BBB [65].…”