In vivo detection limits of magnetically labeled embryonic stem cells in the rat brain using high-field (17.6 T) magnetic resonance imaging

Stroh, Albrecht; Faber, Cornelius; Neuberger, Thomas; Lorenz, Peer; Sieland, Katharina; Jakob, Peter M.; Webb, Andrew; Pilgrimm, Herbert; Schober, R.; Pohl, Elena E.; Zimmer, Claus

doi:10.1016/j.neuroimage.2004.09.014

Cited by 104 publications

(84 citation statements)

References 33 publications

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“…In this system, the absorption of plasma protein on the surface of nanoparticles causes their detection, clearance, and digestion by RES system macrophages. 59 Nanomaterial toxicity in drug delivery systems Nanostructures can be used to transmit drug targets (as a drug or transmitter) or increase drug effectiveness. 60 Nanotechnology is important, especially for detecting and treating cancer, although many problems have yet to be solved.…”

Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Nanotoxicology and nanoparticle safety in biomedical designs

Biazar²,

Jafarpour³

et al. 2011

IJN

153

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Nanotechnology has wide applications in many fields, especially in the biological sciences and medicine. Nanomaterials are applied as coating materials or in treatment and diagnosis. Nanoparticles such as titania, zirconia, silver, diamonds, iron oxides, carbon nanotubes, and biodegradable polymers have been studied in diagnosis and treatment. Many of these nanoparticles may have toxic effects on cells. Many factors such as size, inherent properties, and surface chemistry may cause nanoparticle toxicity. There are methods for improving the performance and reducing toxicity of nanoparticles in medical design, such as biocompatible coating materials or biodegradable/biocompatible nanoparticles. Most metal oxide nanoparticles show toxic effects, but no toxic effects have been observed with biocompatible coatings. Biodegradable nanoparticles are also used in the efficient design of medical materials, which will be reviewed in this article.

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Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Nanotoxicology and nanoparticle safety in biomedical designs

Biazar²,

Jafarpour³

et al. 2011

IJN

153

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“…[39][40][41][42][43][44] Earlier produced as VSOP-C184 (Ferropharm, Teltow, Germany), 45 these have been used for MSC labeling and found to have a detection limit of 40,000 cells in an 11.7 T MRI setting. 46 These NPs are no longer available.…”

Section: Introductionmentioning

confidence: 99%

Labeling of mesenchymal stem cells for MRI with single-cell sensitivity

Schellenberger¹,

Kratz²,

Farr³

et al. 2016

IJN

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Sensitive cell detection by magnetic resonance imaging (MRI) is an important tool for the development of cell therapies. However, clinically approved contrast agents that allow single-cell detection are currently not available. Therefore, we compared very small iron oxide nanoparticles (VSOP) and new multicore carboxymethyl dextran-coated iron oxide nanoparticles (multicore particles, MCP) designed by our department for magnetic particle imaging (MPI) with discontinued Resovist ® regarding their suitability for detection of single mesenchymal stem cells (MSC) by MRI. We achieved an average intracellular nanoparticle (NP) load of >10 pg Fe per cell without the use of transfection agents. NP loading did not lead to significantly different results in proliferation, colony formation, and multilineage in vitro differentiation assays in comparison to controls. MRI allowed single-cell detection using VSOP, MCP, and Resovist ® in conjunction with high-resolution T2*-weighted imaging at 7 T with postprocessing of phase images in agarose cell phantoms and in vivo after delivery of 2,000 NP-labeled MSC into mouse brains via the left carotid artery. With optimized labeling conditions, a detection rate of ~45% was achieved; however, the experiments were limited by nonhomogeneous NP loading of the MSC population. Attempts should be made to achieve better cell separation for homogeneous NP loading and to thus improve NP-uptake-dependent biocompatibility studies and cell detection by MRI and future MPI. Additionally, using a 7 T MR imager equipped with a cryocoil resulted in approximately two times higher detection. In conclusion, we established labeling conditions for new high-relaxivity MCP, VSOP, and Resovist ® for improved MRI of MSC with single-cell sensitivity.

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“…This method allowed us to detect a minimum number of about 500 intrastriatal grafted NSCs, a number slightly higher than that detected by other non-invasive techniques such as magnetic resonance imaging (MRI). Although Stroh et al 19 reported the detection of approximately 10 magnetically labeled cells using MRI, this neuroimaging technique does not allow the quantification of transplanted cells or the evaluation of their proliferation capacity. 19 The advantage of luciferase labeling is that the number of light photons produced by the labeled cells can be used to determine their number.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotection by GDNF-secreting stem cells in a Huntington's disease model: optical neuroimage tracking of brain-grafted cells

et al. 2006

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The use of stem cells for reconstructive or neuroprotective strategies can benefit from new advances in neuroimaging techniques to track grafted cells. In the present work, we analyze the potential of a neural stem cell (NSC) line, which stably expresses the glial cell line-derived neurotrophic factor (GDNF) and the firefly luciferase gene (GDNF/Luc-NSC), for cell therapy in a Huntington's disease mouse model. Our results show that detection of light photons is an effective method to quantify the proliferation rate and to characterize the migration pathways of transplanted NSCs. Intravenous administration of luciferine, the luciferase substract, into the grafted animals allowed the detection of implanted cells in real time by an optical neuroimaging methodology, overpassing the limits of serial histological analyses. We observed that transplanted GDNF/Luc-NSCs survive after grafting and expand more when transplanted in quinolinatelesioned nude mouse striata than when transplanted in nonlesioned mice. We also demonstrate that GDNF/Luc-NSCs prevent the degeneration of striatal neurons in the excitotoxic mouse model of Huntington's disease and reduce the amphetamine-induced rotational behavior in mice bearing unilateral lesions.

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In vivo detection limits of magnetically labeled embryonic stem cells in the rat brain using high-field (17.6 T) magnetic resonance imaging

Cited by 104 publications

References 33 publications

Nanotoxicology and nanoparticle safety in biomedical designs

Nanotoxicology and nanoparticle safety in biomedical designs

Labeling of mesenchymal stem cells for MRI with single-cell sensitivity

Neuroprotection by GDNF-secreting stem cells in a Huntington's disease model: optical neuroimage tracking of brain-grafted cells

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