In Vivo Detection of Hydrogen Sulfide in Brain and Cell

Abstract

Hydrogen sulfide (H 2 S), is proposed as a cytoprotectant and gasotransmitter, involving in many physiological processes and regulating of some diseases. In addition, H 2 S is a small molecular with a minimum of steric hindrance compared with other reactive sulfur species. In physiological atmosphere, H 2 S is mainly existent in HS À , which has a strong nucleophilicity and reducing potency. It also can precipitate with some metal ions forming metallic sulfides with high precipitation coefficient. In recent years, the researchers have a desire to develop methods to achieve real-time detection of H 2 S in vivo, further understanding the physiology and pathology of H 2 S. In this minireview, we summarize recent progress for detecting of H 2 S in brain or cell and briefly expound the principle of methods with the comparison of the different methods between performance and temporal resolution.

Electrochemical Detection of Gasotransmitters: Status and Roadmap

“…This CNF scaffolding also facilitated conductivity and enabled easy application to electrode surfaces. 172 The resulting sensor detected H 2 S within a linear concentration range of 12.5 nM−1.2 mM with an LOD of 1 nM, making it one of the most sensitive electrochemical devices reported recently for the detection of H 2 S. 173 In another recent example, gold nanowires were incorporated with carbon nanotubes to form an electrocatalyst on a flexible poly(dimethylsiloxane) (PDMS) substrate. This stretchable sensor was able to detect H 2 S with a broad linear range from 5 nM to 24.9 μM and successfully monitored the release of H 2 S from HeLa cells cultured on the flexible sensor.…”