In vivo determination of endogenous biogenic amines in rat brain using HPLC and push-pull cannula

Loullis, Costas C.; Hingtgen, J. N.; Shea, P.A.; Aprison, M. H.

doi:10.1016/0091-3057(80)90459-1

Cited by 60 publications

(17 citation statements)

References 10 publications

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“…without further purification as described by Loullis et al (1980) and Lyness (1982). Striatal tissues were homogenized by ultrasonication in 400 pl of 0.2 M perchloric acid containing 0.11 M asorbic acid and centrifuged.…”

Section: Methodsmentioning

confidence: 99%

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Release of endogenous dopamine from rat isolated striatum: effect of clorgyline and (−)−deprenyl

Hársing

Vizi

1984

British J Pharmacology

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1 High performance liquid chromatography with electrochemical detection was used to measure the release and content of dopamine and dihydroxyphenylacetic acid (DOPAC) from rat isolated striatum. The effects of the monoamine oxidase (MAO) inhibitors clorgyline and (-)-deprenyl on dopamine and DOPAC release and contents, and the IC50 values of these compounds for inhibition of dopamine deamination in rat striatum were determined. 2 Dopamine release was significantly increased by elevated KCl (22 mM) in a Ca2+-dependent manner, and by ouabain (20 LM), whereas the release of DOPAC remained constant. The loss in striatal dopamine content during the incubation period (67 % of initial content) was far greater than the amount of dopamine recovered in the incubation fluid (16% of initial content), suggesting that much of the DOPAC, released during incubation originated from the conversion of dopamine to DOPAC within the striatum. 3 A concentration-dependent decrease in DOPAC efflux, both during rest and stimulation periods, was observed in the presence of clorgyline (10-8M-10-7M) and (-)-deprenyl (10 5M-10 4M). 4 Higher concentrations of clorgyline (10-7M) and (-)-deprenyl (10-4M), which inhibited dopamine deamination by 85-90%, enhanced both the resting and KCl-induced release of dopamine. 5 The total amount of dopamine plus DOPAC that was released in the presence of clorgyline or (-)-deprenyl did not differ from control values, suggesting that the increase in dopamine release elicited by MAO inhibitors might result from reduced degradation of dopamine to DOPAC. 6 The IC50 values of clorgyline (5 x 10-9M) and (-)-deprenyl (5 x 10-6M) for inhibition of dopamine deamination indicate that dopamine is a substrate for type A MAO in rat striatum. IntroductionIt is generally accepted that dopamine released from axon terminal varicosities of the nigrostriatal pathway has an important role in the regulation of striatal neurotransmission (Vizi et al., 1977;Dray, 1979;Lehmann & Langer, 1983). Yet the release of endogenous dopamine as a possible index of dopaminergic neural activity is not commonly measured because of the small amount of dopamine released from brain slice preparations. Drugs affecting the release of dopamine in the striatum have been investigated mainly by indirect methods such as measurement of [3H]-dopamine synthesis from [3H]-tyrosine (Glowinski, 1976) (Starke et al., 1978; Cubeddu & Hoffman, 1982). Studies with the [3H]-dopamine method, however, have often produced conflicting data. For example, when the effects of opioid peptides were studied, it was observed in one study that Pendorphin but not Met enkephalin, inhibited striatal dopamine release (Loh etal., 1976), whereas in other studies, 1-endorphin was reported to have no effect (Arbilla & Langer, 1978) and Met enkephalin was found to be inhibitory on dopamine release (Subramanian et al., 1977). Similarly, studies of the effects of neuroleptics on striatal dopamine release have yielded contradictory data (Farnebo & Hamberger, 1971;Seeman & Lee, 1975).I...

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Section: Methodsmentioning

confidence: 99%

“…without further purification as described by Loullis et al (1980) and Lyness (1982 Mobile phase consisted of 0.1 M sodium acetate/citric acid, pH 4, containing 8% methanol and 0.4 mm sodium octyl sulphate. The mobile phase was filtered then degassed in an ultrasonic generator.…”

Section: Methodsmentioning

confidence: 99%

Release of endogenous dopamine from rat isolated striatum: effect of clorgyline and (−)−deprenyl

Hársing

Vizi

1984

British J Pharmacology

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“…However, this technique also has disadvantages. Thus, until recently (Loullis et al, 1980;Nielsen & Moore, 1982), push-pull studies invariably involved anaesthetized animals so that parallel behavioural and biochemical measurements were impossible. Also the method is technically difficult; there are problems in balancing the push and pull perfusion pressures, otherwise excessive tissue damage can occur (Redgrave, 1977).…”

Section: Introductionmentioning

confidence: 99%

“…211 into transmitters and metabolites (Glowinski, 1981). Assay problems have been recently overcome by the use of high pressure liquid chromatography with electrochemical detection (Loullis et al, 1980). However, another problem remains which is intrinsic to push-pull methods, that is they may disturb relationships between transmitter release and reuptake.…”

Section: Introductionmentioning

confidence: 99%

Monitoring in vivo of transmitter metabolism by electrochemical methods

Hutson¹,

Curzon²

1983

Biochemical Journal

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IntroductionConventional methods of studying the metabolism of central neurotransmitter amines (i.e. dopamine, noradrenaline and 5-hydroxytryptamine) in rats and other small laboratory animals have two major disadvantages. Firstly, animals under investigation must be killed before determinations can be made and secondly, intra-and extra-neuronal metabolism are not differentiated. Thus, determinations can only be made at one time per animal and information is not obtained on whether transmitters or metabolites measured reflect functional activity, i.e. transmitter molecules which had been available to receptors. Also, standard methods of measuring transmitter turnover cannot be applied to individual rats but require the use of groups of animals on which changes of amine, amine precursor or metabolite concentration are measured over a period after the administration of drugs which block transmitter synthesis or catabolism or egress of amine metabolites from the central nervous system.

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“…The extracts were assayed (by Drs J. R. Simon and P. A. Shea) for 5-HT, noradrenaline, adrenaline and dopamine by high pressure liquid chromatography, as described by Loullis, Hingtgen, Shea & Aprison (1980), after precipitation of excess acid with K2 CO8. They were also assayed (by Professor W. Lorenz) for histamine, by fluorimetry following condensation of the histamine with orthophthaldialdehyde (Shore, Burkhalter & Cohn, 1959;Lorenz, Matejka, Schmal, Seidel, Reimann, Uhlig & Mann, 1973).…”

Section: Assay Of Aminesmentioning

confidence: 99%

Constrictor actions of acetylcholine, 5‐hydroxytryptamine and histamine on bovine coronary artery inner and outer muscle

Garland

Keatinge

1982

The Journal of Physiology

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SUMMARY1. In bovine coronary arteries, cholinesterase staining showed an extensive cholinergic innervation at the adventitia-media junction, and some cholinesterase in the outer but not inner smooth muscle.2. Acetylcholine or methacholine caused large, atropine-sensitive contractions of outer muscle but caused little contraction of inner muscle.3. Fluorescence microscopy for monoamines and for histamine, supported by chemical assays, showed no adrenergic innervation but showed numerous fluorescent cells in the adventitia and the outer 50 % of the media which stained as mast cells and contained large amounts of histamine and noradrenaline and some dopamine, but little 5-hydroxytryptamine (5-HT).4. 5-hydroxytryptamine (acting by D receptors) and histamine (acting by H1 receptors) in high concentrations caused large contractions, of similar size, in inner and outer muscle. In given submaximal concentrations they generally caused more contraction ofouter than inner muscle, particularly in the case of histamine, provided that imipramine or desipramine was present to inhibit uptake of the agents by mast cells which were present in the outer part of the artery wall.5. Without blockade of uptake, 5-HT applied to the arteries in submaximal concentrations caused less contraction of outer than inner muscle; histamine still caused significantly more contraction of outer than inner muscle.6. The findings indicate that the cholinergic constrictor nerves of these arteries, unlike adrenergic constrictor nerves of other systemic arteries, act almost solely on outer muscle of the vessel wall; and that mast cells give considerable protection against constriction by 5-HT, but little against histamine, reaching the vessel from its adventitial surface.

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In vivo determination of endogenous biogenic amines in rat brain using HPLC and push-pull cannula

Cited by 60 publications

References 10 publications

Release of endogenous dopamine from rat isolated striatum: effect of clorgyline and (−)−deprenyl

Release of endogenous dopamine from rat isolated striatum: effect of clorgyline and (−)−deprenyl

Monitoring in vivo of transmitter metabolism by electrochemical methods

Constrictor actions of acetylcholine, 5‐hydroxytryptamine and histamine on bovine coronary artery inner and outer muscle

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