In Vivo Discography in Degenerate Porcine Spines Revealed Pressure Transfer to Adjacent Discs

Hebelka, Hanna; Nilsson, Andreas; Ekström, Lars; Hansson, Tommy

doi:10.1097/01.brs.0000435141.61593.05

Cited by 11 publications

(7 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Smaller sized animals such as the mouse and rat (recently Bailey et al, ; Holguin et al, ; Jin et al, ; Miyagi et al, ; Pelle et al, ) as well as larger animals such as the pig (recently Ivicsics et al, ; Hebelka et al, ; Yilgor et al, ), sheep (recently Reitmaier et al, ; Wade et al, ), and cow tail (recently Chan and Gantenbein‐Ritter, ; Eder et al, ; Farrell and Riches, ) have been extensively used in the literature. Substantial work has also been conducted on verifying the suitability of such animal models for the study of IVD disorders (Wilke et al, ; Beckstein et al, ; Neidlinger‐Wilke et al, ; Ohtori et al, ; comprehensive reviews by Alini et al, and Singh et al, ).…”

Section: Introductionmentioning

confidence: 99%

A comparison between porcine, ovine, and bovine intervertebral disc anatomy and single lamella annulus fibrosus tensile properties

Monaco

DeWitte‐Orr

Gregory

2015

Journal of Morphology

View full text Add to dashboard Cite

This project aimed to compare gross anatomical measures and biomechanical properties of single lamellae from the annulus fibrosus of ovine and porcine lumbar vertebrae, and bovine tail vertebrae. The morphology of the vertebrae of these species differ significantly both from each other and from human, yet how these differences alter biomechanical properties is unknown. Geometric parameters measured in this study included: 1) absolute and relative intervertebral (IVD) and vertebral body height and 2) absolute and relative intervertebral disc (IVD) anterior-posterior (AP) and medial-lateral (ML) widths. Single lamella tensile properties included toe-region stress and stretch ratio, stiffness, and tensile strength. As expected, the bovine tail IVD revealed a more circular shape compared with both the ovine and porcine lumbar IVD. The bovine tail also had the largest IVD to vertebral body height ratio (due to having the highest absolute IVD height). Bovine tail lamellae were also found to be strongest and stiffest (in tension) while ovine lumbar lamellae were weakest and most compliant. Histological analysis revealed the greatest proportion of collagen in the bovine corroborating findings of increased strength and stiffness. The observed differences in anatomical shape, connective tissue composition, and tensile properties need to be considered when choosing an appropriate model for IVD research.

show abstract

Section: Introductionmentioning

confidence: 99%

A comparison between porcine, ovine, and bovine intervertebral disc anatomy and single lamella annulus fibrosus tensile properties

Monaco

DeWitte‐Orr

Gregory

2015

Journal of Morphology

View full text Add to dashboard Cite

show abstract

“…It has also been suggested that the injected contrast agent in itself may cause accelerated degeneration by inducing cell apoptosis [44]. In addition, it was recently demonstrated that discography-induced pressure increases not only in the injected disc, but also in adjacent discs potentially inducing false-positive responses [8][9][10]. This strongly questions the validity of discography as a tool-provoking pain on a single disc level only.…”

Section: Discussionmentioning

confidence: 97%

“…Discography remains, however, questioned due to factors such as inconclusiveness regarding false-positive responses, lack of ''gold standard'' and uncertainty of what actually causes the pain in or around the disc [3][4][5][6][7]. A recent finding that discography induces a significant pressure increase not only in the injected disc, but also in adjacent discs that further raises new questions about discography's validity [8][9][10].…”

Section: Introductionmentioning

confidence: 98%

Comparison between pain at discography and morphological disc changes at axial loaded MRI in patients with low back pain

2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…To our knowledge, this has only been developed for mice and dogs . In terms of imaging, large animal models have rather similar possibilities as humans with LBP: radiography, fluoroscopy, discography, computer tomography (CT), and magnetic resonance imaging (MRI) . However, there are some drawbacks.…”

Section: Preclinical Animal Modelsmentioning

confidence: 99%

“…123 In terms of imaging, large animal models have rather similar possibilities as humans with LBP: radiography, fluoroscopy, discography, computer tomography (CT), and magnetic resonance imaging (MRI). 67,90,124,125 However, there are some drawbacks. For instance, quantitative MRI (eg, T1rho and T2 mapping) has been validated for human IVD degeneration, 126,127 but not for animals.…”

Section: Differences In Ivds Between Human and Animal Species Includementioning

confidence: 99%

Leaping the hurdles in developing regenerative treatments for the intervertebral disc from preclinical to clinical

et al. 2018

View full text Add to dashboard Cite

Chronic back and neck pain is a prevalent disability, often caused by degeneration of the intervertebral disc. Because current treatments for this condition are less than satisfactory, a great deal of effort is being applied to develop new solutions, including regenerative strategies. However, the path from initial promising idea to clinical use is fraught with many hurdles to overcome. Many of the keys to success are not necessarily linked to science or innovation. Successful translation to clinic will also rely on planning and awareness of the hurdles. It will be essential to plan your entire path to clinic from the outset and to do this with a multidisciplinary team. Take advice early on regulatory aspects and focus on generating the proof required to satisfy regulatory approval. Scientific demonstration and societal benefits are important, but translation cannot occur without involving commercial parties, which are instrumental to support expensive clinical trials. This will only be possible when intellectual property can be protected sufficiently to support a business model. In this manner, commercial, societal, medical, and scientific partners can work together to ultimately improve patient health. Based on literature surveys and experiences of the co‐authors, this opinion paper presents this pathway, highlights the most prominent issues and hopefully will aid in your own translational endeavors.

show abstract

In Vivo Discography in Degenerate Porcine Spines Revealed Pressure Transfer to Adjacent Discs

Abstract: Discography induced pressure increase in adjacent discs in a degenerate disc model, something not reported earlier. If present, also in human spine pressure transmission, may be a potential cause for false positive discography responses.

Cited by 11 publications

References 47 publications

A comparison between porcine, ovine, and bovine intervertebral disc anatomy and single lamella annulus fibrosus tensile properties

A comparison between porcine, ovine, and bovine intervertebral disc anatomy and single lamella annulus fibrosus tensile properties

Comparison between pain at discography and morphological disc changes at axial loaded MRI in patients with low back pain

Leaping the hurdles in developing regenerative treatments for the intervertebral disc from preclinical to clinical

Contact Info

Product

Resources

About