In Vivo Distribution of Poly(ethylene glycol) Functionalized Iron Oxide Nanoclusters: An Ultrastructural Study

Suciu, Maria; Mirescu, Claudiu; Crăciunescu, I.; Macavei, Sergiu; Leoștean, Cristian; Ştefan, R.; Olar, Loredana; Tripon, Septimiu-Cassian; Ciorîță, Alexandra; Barbu–Tudoran, Lucian

doi:10.3390/nano11092184

Cited by 8 publications

(4 citation statements)

References 93 publications

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“…The results were in accordance with other studies, where PEG-coated nanoparticles (less than 50 nm) showed saturation magnetization (Ms) of 23 emu/g with Mr values less than 2 nm [ 45 , 46 ]. Another study with a similar size distribution of PEG-coated nanoparticles (50–300 nm) also showed superparamagnetic behavior with a coercivity value of 27 Oe and an Mr of 2.2 [ 47 ]. The current study with a particle size of 190 nm showed a coercivity of 19.06 Oe and an Mr of 0.125 emu/g.…”

Section: Resultsmentioning

confidence: 98%

Novel Magnetic Elastic Phase-Change Nanodroplets as Dual Mode Contrast Agent for Ultrasound and Magnetic Resonance Imaging

Riaz

Waqar²,

Ahmad

et al. 2022

Polymers

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Recently, dual-mode imaging systems merging magnetic resonance imaging (MRI) and ultrasound (US) have been developed. Designing a dual-mode contrast agent is complex due to different mechanisms of enhancement. Herein, we describe novel phase change nanodroplets (PCNDs) with perfluoropentane encapsulated in a pre-polyglycerol sebacate (pre-PGS) shell loaded with polyethylene glycol (PEG)-coated iron oxide nanoparticles as having a dual-mode contrast agent effect. Iron oxide nanoparticles were prepared via the chemical co-precipitation method and PCNDs were prepared via the solvent displacement technique. PCNDs showed excellent enhancement in the in vitro US much more than Sonovue® microbubbles. Furthermore, they caused a susceptibility effect resulting in a reduction of signal intensity on MRI. An increase in the concentration of nanoparticles caused an increase in the MR contrast effect but a reduction in US intensity. The concentration of nanoparticles in a shell of PCNDs was optimized to obtain a dual-mode contrast effect. Biocompatibility, hemocompatibility, and immunogenicity assays showed that PCNDs were safe and non-immunogenic. Another finding was the dual-mode potential of unloaded PCNDs as T1 MR and US contrast agents. Results suggest the excellent potential of these PCNDs for use as dual-mode contrast agents for both MRI and US.

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Section: Resultsmentioning

confidence: 98%

Novel Magnetic Elastic Phase-Change Nanodroplets as Dual Mode Contrast Agent for Ultrasound and Magnetic Resonance Imaging

Riaz

Waqar²,

Ahmad

et al. 2022

Polymers

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“…Dehydrated samples were then embedded in Epon 812 resin, gradually, using Epon in acetone solutions at ratios of 1:3, 1:2; 1:1, 2:1, and 3:1 (1 h each step)m alogn with pure Epon (overnight). Embedded samples were placed in plastic capsules and left to cure at 60 °C for 2–3 days until hard (polymerized) [ 17 ]. Capsules were then cut off, the hardened samples were trimmed and sectioned using Leica UC7 Ultramicrotome, obtaining 150 nm and 50–90 nm thick sections.…”

Section: Methodsmentioning

confidence: 99%

Ultrastructure of the Bovine Testis in Cattle (Bos taurus): New View

Michałek,

Grabowska,

Oberska

et al. 2024

Animals

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The purpose of this study was to analyze the ultrastructure of the testes of sexually immature calves and reproductive bulls of the Polish Holstein-Friesian Black-and-White breed. Utilizing TEM, this study identified three distinct stages of seminiferous tubule development in calves, characterized by varying shapes, distributions, and arrangements of individual cells. In immature animals, early developing spermatocytes, prespermatogonia, and pre-Sertoli cells were observed within the seminiferous tubules. In sexually mature bulls, all cells of the spermatogenic series were observed, situated on a thin, multilayered basal lamina, which forms characteristic undulations. An abundant smooth endoplasmic reticulum was observed in the cytoplasm of spermatogonia in both groups of animals, forming characteristic membranous swirls. In adult bulls, spermatogonia maintain contact with each other through numerous cytoplasmic bridges and cell connections, forming small spaces with visible microvilli between them. The ultrastructural analysis facilitated the identification of morphological changes occurring during the maturation of pre-Sertoli cells, transitioning from a large euchromatic nucleus to a nucleus in which the formation of characteristic vesicles and tubules could be observed. It should also be emphasized that two types of Sertoli cells, namely dark and light electron-dense cells, can be found in cattle. These cells differ from each other, indicating that they may perform different functions. The widespread recognition of the presence of two types of Sertoli cells in cattle will undoubtedly contribute to a better understanding of the processes occurring within the testes and provide a basis for further research in this area.

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“…Sorted by crystalline features, metal NMs could be classified into representative references, based on pure-phased crystalline such as nanoparticles (NPs) with highly crystalline properties and metal clusters formed by atoms aggregations [ 9 ]. When their properties were evaluated in physicochemical works, metal clusters drew the most interest among composite materials due to their unique characteristics [ 10 , 11 , 12 , 13 ]. Nanoclusters consisting of fewer atoms have a higher specific surface area, which might perform the promised function actively.…”

Section: Introductionmentioning

confidence: 99%

Metal Cluster Triggered-Assembling Heterogeneous Au-Ag Nanoclusters with Highly Loading Performance and Biocompatible Capability

Yang

et al. 2022

IJMS

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In this work, we firstly report the preparation of heterogeneously assembled structures Au-Ag nanoclusters (NCs) as good drug carriers with high loading performance and biocompatible capability. As glutathione-protected Au and Ag clusters self-assembled into porous Au-Ag NCs, the size value is about 1.358 (±0.05) nm. The morphology characterization revealed that the diameter of Au-Ag NCs is approximately 120 nm, as well as the corresponding potential ability in loading performance of the metal cluster triggered-assembling process. Compared with individual components, the stability and loading performance of heterogeneous Au-Ag NCs were improved and exhibit that the relative biocompatibility was enhanced. The exact information about this is that cell viability was approximately to 98% when cells were incubated with 100 µg mL−1 particle solution for 3 days. The drug release of Adriamycin from Au-Ag NCs was carried out in PBS at pH = 7.4 and 5.8, respectively. By simulating in vivo and tumor microenvironment, the release efficiency could reach over 65% at pH = 5.8 but less than 30% at pH = 7.2. Using an ultrasound field as external environment can accelerate the assembling process while metal clusters triggered assembling Au-Ag NCs. The size and morphology of the assembled Au-Ag NCs can be controlled by using different power parameters (8 W, 13 W, 18 W) under ambient atmosphere. Overall, a novel approach is exhibited, which conveys assembling work for metal clusters triggers into heterogeneous structures with porous characteristic. Its existing properties such as water-solubility, stability, low toxicity and capsulation can be considered as dependable agents in various biomedical applications and drug carriers in immunotherapies.

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In Vivo Distribution of Poly(ethylene glycol) Functionalized Iron Oxide Nanoclusters: An Ultrastructural Study

Cited by 8 publications

References 93 publications

Novel Magnetic Elastic Phase-Change Nanodroplets as Dual Mode Contrast Agent for Ultrasound and Magnetic Resonance Imaging

Novel Magnetic Elastic Phase-Change Nanodroplets as Dual Mode Contrast Agent for Ultrasound and Magnetic Resonance Imaging

Ultrastructure of the Bovine Testis in Cattle (Bos taurus): New View

Metal Cluster Triggered-Assembling Heterogeneous Au-Ag Nanoclusters with Highly Loading Performance and Biocompatible Capability

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