In Vivo Electrochemical Studies of Optogenetic Control of Glutamate Signaling Measured Using Enzyme-Based Ceramic Microelectrode Arrays

Burmeister, Jason J.; Pomerleau, François; Quintero, Jorge E.; Huettl, Peter; Ai, Yi; Jakobsson, Johan; Lundblad, Martin; Heuer, Andreas; Slevin, John T.; Gerhardt, Greg A.

doi:10.1007/978-1-4939-7228-9_11

Cited by 8 publications

(7 citation statements)

References 56 publications

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“…Direct assessment of neurotransmission provides for simultaneous measurement with other measurements of neuronal activity with millisecond precision. Emerging approaches involve the design of microelectrodes capable of concurrent assessment of neural electrical activity with local field potentials (LFPs) [196,197]. The integration of neurochemical information with electrophysiological approaches will greatly expand our understanding of the circuit-neurotransmitter relationship.…”

Section: Moving Into the Future: Recordings In Freely-moving Animalsmentioning

confidence: 99%

“…Specifically, direct measurement of brain chemical signaling provides valuable information when combined with other physiological methods to manipulate neuronal activity with sophisticated observational work and casual manipulation studies. Targeting neuronal systems infers causality between neurotransmitter events and behavior in conjunction with optogenetic-based [197] or chemogenetic (DREADD) approaches [200]. Optogenetics combined with in vivo electrochemical assessments has already proven to be effective in studying glutamate signaling in both hippocampus and frontal cortex in anesthetized animals [197,201].…”

Section: Moving Into the Future: Recordings In Freely-moving Animalsmentioning

confidence: 99%

“…Targeting neuronal systems infers causality between neurotransmitter events and behavior in conjunction with optogenetic-based [197] or chemogenetic (DREADD) approaches [200]. Optogenetics combined with in vivo electrochemical assessments has already proven to be effective in studying glutamate signaling in both hippocampus and frontal cortex in anesthetized animals [197,201]. This is particularly important for the study of circuit-level mechanisms of disease in vivo, when applied to existing behavioral models as output measures can provide insights for future clinical translation.…”

Section: Moving Into the Future: Recordings In Freely-moving Animalsmentioning

confidence: 99%

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Approaches to Monitor Circuit Disruption after Traumatic Brain Injury: Frontiers in Preclinical Research

Krishna

Beitchman

Bromberg

et al. 2020

IJMS

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Mild traumatic brain injury (TBI) often results in pathophysiological damage that can manifest as both acute and chronic neurological deficits. In an attempt to repair and reconnect disrupted circuits to compensate for loss of afferent and efferent connections, maladaptive circuitry is created and contributes to neurological deficits, including post-concussive symptoms. The TBI-induced pathology physically and metabolically changes the structure and function of neurons associated with behaviorally relevant circuit function. Complex neurological processing is governed, in part, by circuitry mediated by primary and modulatory neurotransmitter systems, where signaling is disrupted acutely and chronically after injury, and therefore serves as a primary target for treatment. Monitoring of neurotransmitter signaling in experimental models with technology empowered with improved temporal and spatial resolution is capable of recording in vivo extracellular neurotransmitter signaling in behaviorally relevant circuits. Here, we review preclinical evidence in TBI literature that implicates the role of neurotransmitter changes mediating circuit function that contributes to neurological deficits in the post-acute and chronic phases and methods developed for in vivo neurochemical monitoring. Coupling TBI models demonstrating chronic behavioral deficits with in vivo technologies capable of real-time monitoring of neurotransmitters provides an innovative approach to directly quantify and characterize neurotransmitter signaling as a universal consequence of TBI and the direct influence of pharmacological approaches on both behavior and signaling.

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Section: Moving Into the Future: Recordings In Freely-moving Animalsmentioning

confidence: 99%

Section: Moving Into the Future: Recordings In Freely-moving Animalsmentioning

confidence: 99%

Section: Moving Into the Future: Recordings In Freely-moving Animalsmentioning

confidence: 99%

See 1 more Smart Citation

Approaches to Monitor Circuit Disruption after Traumatic Brain Injury: Frontiers in Preclinical Research

Krishna

Beitchman

Bromberg

et al. 2020

IJMS

View full text Add to dashboard Cite

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“…However, being able to finely tune defined circuits is still challenging but strides are being made for therapeutic applications and adjustments in behavior [ 1 – 3 ]. Recent approaches are advancing in the use of flexible and very thin optic fibers to control neurons in deep regions of the CNS in model animals by the use of light activated proteins (i.e., optogenetics) with good spatial and temporal resolution [ 4 – 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…Direct electrophysiological measures across cell membranes are feasible in model systems for drawing inference to more complex systems such as the human brain. Bridging optogenetic control of cellular activity and the direct measurement of synaptic responses provides a clear understanding of how intact circuits are functioning and if the experimental paradigms with an optogenetic drive are producing the desired outcomes [ 8 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Hyperpolarization by activation of halorhodopsin results in enhanced synaptic transmission: Neuromuscular junction and CNS circuit

et al. 2018

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Optogenetics offers a unique method to regulate the activity of select neural circuits. However, the electrophysiological consequences of targeted optogenetic manipulation upon the entire circuit remain poorly understood. Analysis of the sensory-CNS-motor circuit in Drosophila larvae expressing eHpHR and ChR2-XXL revealed unexpected patterns of excitability. Optical stimulation of motor neurons targeted to express eNpHR resulted in inhibition followed by excitation of body wall contraction with repetitive stimulation in intact larvae. In situ preparations with direct electrophysiological measures showed an increased responsiveness to excitatory synaptic activity induced by sensory stimulation within a functional neural circuit. To ensure proper function of eNpHR and ChR2-XXL they were expressed in body wall muscle and direct electrophysiological measurements were obtained. Under eNpHR induced hyperpolarization the muscle remained excitable with increased amplitude of excitatory postsynaptic synaptic potentials. Theoretical models to explain the observations are presented. This study aids in increasing the understanding of the varied possible influences with light activated proteins within intact neural circuits.

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Miniaturized Electrochemical Sensing Platforms for Quantitative Monitoring of Glutamate Dynamics in the Central Nervous System

Wang,

Yang,

Chen

et al. 2024

Angewandte Chemie

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Glutamate is one of the most important excitatory neurotransmitters within the mammalian central nervous system. The role of glutamate in regulating neural network signaling transmission through both synaptic and extra‐synaptic paths highlights the importance of the real‐time and continuous monitoring of its concentration and dynamics in living organisms. Progresses in multidisciplinary research have promoted the development of electrochemical glutamate sensors through the co‐design of materials, interfaces, electronic devices, and integrated systems. This review summarizes recent works reporting various electrochemical sensor designs and their applicability as miniaturized neural probes to in vivo sensing within biological environments. We start with an overview of the role and physiological significance of glutamate, the metabolic routes, and its presence in various bodily fluids. Next, we discuss the design principles, commonly employed validation models/protocols, and successful demonstrations of multifunctional, compact, and bio‐integrated devices in animal models. The final section provides an outlook on the development of the next generation glutamate sensors for neuroscience and neuroengineering, with the aim of offering practical guidance for future research.

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In Vivo Electrochemical Studies of Optogenetic Control of Glutamate Signaling Measured Using Enzyme-Based Ceramic Microelectrode Arrays

Cited by 8 publications

References 56 publications

Approaches to Monitor Circuit Disruption after Traumatic Brain Injury: Frontiers in Preclinical Research

Approaches to Monitor Circuit Disruption after Traumatic Brain Injury: Frontiers in Preclinical Research

Hyperpolarization by activation of halorhodopsin results in enhanced synaptic transmission: Neuromuscular junction and CNS circuit

Miniaturized Electrochemical Sensing Platforms for Quantitative Monitoring of Glutamate Dynamics in the Central Nervous System

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