In vivo engineered extracellular matrix scaffolds with instructive niches for oriented tissue regeneration

Zhu, Meifeng; Li, Wen; Dong, Xianhao; Yuan, Xingyu; Midgley, Adam C.; Chang, Hong; Wang, Yuhao; Wang, Haoyu; Wang, Kai; Peter, X.; Wang, Hongjun; Kong, Deling

doi:10.1038/s41467-019-12545-3

Cited by 239 publications

(172 citation statements)

References 63 publications

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“…Nowadays, three-dimensional (3D) scaffolds are deeply investigated for their ability to guide cell fate [1,2]. In fact, biocompatible scaffolds have been developed to mimic the physiological microenvironment of specific cells and their growth inside these scaffolds typically allows a cellular response more representative of the in vivo behavior with respect to the 2D cell culture conditions [3][4][5][6]. For this reason, the use of 3D scaffolds is important in the field of regenerative medicine in order to improve stem cells therapeutic effect [7,8].…”

Section: Introductionmentioning

confidence: 99%

Dissecting the Effect of a 3D Microscaffold on the Transcriptome of Neural Stem Cells with Computational Approaches: A Focus on Mechanotransduction

Rey

Pandini

Barzaghini

et al. 2020

IJMS

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3D cell cultures are becoming more and more important in the field of regenerative medicine due to their ability to mimic the cellular physiological microenvironment. Among the different types of 3D scaffolds, we focus on the Nichoid, a miniaturized scaffold with a structure inspired by the natural staminal niche. The Nichoid can activate cellular responses simply by subjecting the cells to mechanical stimuli. This kind of influence results in different cellular morphology and organization, but the molecular bases of these changes remain largely unknown. Through RNA-Seq approach on murine neural precursors stem cells expanded inside the Nichoid, we investigated the deregulated genes and pathways showing that the Nichoid causes alteration in genes strongly connected to mechanobiological functions. Moreover, we fully dissected this mechanism highlighting how the changes start at a membrane level, with subsequent alterations in the cytoskeleton, signaling pathways, and metabolism, all leading to a final alteration in gene expression. The results shown here demonstrate that the Nichoid influences the biological and genetic response of stem cells thorough specific alterations of cellular signaling. The characterization of these pathways elucidates the role of mechanical manipulation on stem cells, with possible implications in regenerative medicine applications.

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Section: Introductionmentioning

confidence: 99%

Dissecting the Effect of a 3D Microscaffold on the Transcriptome of Neural Stem Cells with Computational Approaches: A Focus on Mechanotransduction

Rey

Pandini

Barzaghini

et al. 2020

IJMS

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“…Even these composite materials possess relatively simple composition with two, or three major components, their roles were strictly limited to functions such as enhancing cell adhesion, promoting osteogenic differentiation, etc. during bone regeneration (Wei and Ma, 2004;Zhu et al, 2019). Thus biological materials such as ECM, which contain complex biocompatible composition may be potential candidates for novel bone repairing materials development.…”

Section: Discussionmentioning

confidence: 99%

Utility of Air Bladder-Derived Nanostructured ECM for Tissue Regeneration

Wang

Chen²,

Ran³

et al. 2020

Front. Bioeng. Biotechnol.

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Exploration for ideal bone regeneration materials still remains a hot research topic due to the unmet clinical challenge of large bone defect healing. Bone grafting materials have gradually evolved from single component to multiple-component composite, but their functions during bone healing still only regulate one or two biological processes. Therefore, there is an urgent need to develop novel materials with more complex composition, which convey multiple biological functions during bone regeneration. Here, we report an naturally nanostructured ECM based composite scaffold derived from fish air bladder and combined with dicalcium phosphate (DCP) microparticles to form a new type of bone grafting material. The DCP/acellular tissue matrix (DCP/ATM) scaffold demonstrated porous structure with porosity over 65% and great capability of absorbing water and other biologics. In vitro cell culture study showed that DCP/ATM scaffold could better support osteoblast proliferation and differentiation in comparison with DCP/ADC made from acid extracted fish collagen. Moreover, DCP/ATM also demonstrated more potent bone regenerative properties in a rat calvarial defect model, indicating incorporation of ECM based matrix in the scaffolds could better support bone formation. Taken together, this study demonstrates a new avenue toward the development of new type of bone regeneration biomaterial utilizing ECM as its key components.

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“…54 ECM scaffold derived from cultured cells lacks hierarchical porous structure to provide cells with guidance cues for directional migration and spatial organization. 57 To improve the structure of ECM scaffold, Zhu et al established ECM scaffolds with parallel microchannel in vivo. 57 Sacrificial templates, i.e.…”

Section: Autologous Hydrogels For Vascular Regenerationmentioning

confidence: 99%

Vascular Tissue Engineering: Advanced Techniques and Gene Editing in Stem Cells for Graft Generation

Chen

Ugwu

Li³

et al. 2021

Tissue Engineering Part B: Reviews

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The common occurrence of cardiovascular diseases and the lack of proper autologous tissues prompt and promote the pressing development of tissue-engineered vascular grafts. Current progress on scaffold production, genetically modified cells and use of nanotechnology-based monitoring has considerably improved the long-term patency of engineered tissue grafts. However, challenges abound in the autologous materials and manipulation of genes and cells for tissue engineering. This review overviews current development in tissue-engineered vascular grafts and discusses recent improvements in scaffolding techniques as well as the efficiency of gene-editing tools and their ability to fill the existing gaps in stem-cell and regenerative therapies. Current advances in 3D-printing approaches for fabrication of engineered tissues are also reviewed together with specific biomaterials for vascular tissues. In addition, the natural and synthetic polymers that hold increasing significance for vascular tissue engineering are highlighted. Both animal models and nanotechnology-based monitoring are proposed for pre-clinical evaluation of engineered grafts in view of their historical significance in tissue engineering. The ultimate success of tissue regeneration, which is yet to be fully realized, depends on the optimal performance of culture systems, biomaterial constructs and stem cells in a suitable artificial physiological environment.

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In vivo engineered extracellular matrix scaffolds with instructive niches for oriented tissue regeneration

Cited by 239 publications

References 63 publications

Dissecting the Effect of a 3D Microscaffold on the Transcriptome of Neural Stem Cells with Computational Approaches: A Focus on Mechanotransduction

Dissecting the Effect of a 3D Microscaffold on the Transcriptome of Neural Stem Cells with Computational Approaches: A Focus on Mechanotransduction

Utility of Air Bladder-Derived Nanostructured ECM for Tissue Regeneration

Vascular Tissue Engineering: Advanced Techniques and Gene Editing in Stem Cells for Graft Generation

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