In vivo estrogenic-like activities of Gouania longipetala Hemsl. (Rhamnaceae) bark extracts in a post-menopause-like model of ovariectomized Wistar rats

Désiré, Dzeufiet Djomeni Paul; Sandrine, Mengue Ngadena Yolande; Claude, Bilanda Danielle; Mireille, Kameni Poumeni; Bibi-Farouck, Aboubakar Oumarou; Dimo, Théophile; Kamtchouing, Pièrre

doi:10.1016/j.jep.2015.03.049

Cited by 14 publications

(5 citation statements)

References 27 publications

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“…[ 13 ] The in vitro antioxidant properties of G. longipetala could be a supportive mechanism of the proven biological activities of this plant such as anti-inflammatory, antidiabetic, antilipidemic, and estrogenic effects. [ 13 14 15 ] Furthermore, the plant’s antioxidant potentials could sustain its various ethnopharmacological applications. [ 8 9 10 11 12 ]…”

Section: Discussionmentioning

confidence: 99%

“…[ 8 9 10 11 12 ] Research studies have demonstrated the antibacterial, anti-inflammatory, antidiabetic, antilipidemic, and estrogenic effects of extracts from G. longipetala . [ 13 14 15 ] Such activities of G. longipetala may be due to the antioxidant activity and/or antioxidant content of this plant. In fact, a previous study showed the ameliorative activity of G. longipetala extracts on malondialdehyde levels and superoxide dismutase activities in alloxan-induced diabetic rats.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant content and activity of stem and leaf extracts of Gouania longipetala Hemsl. (Rhamnaceae)

Soh,

Choumessi,

Tsague Manfo

et al. 2022

AYU (An International Quarterly Journal of Research in Ayurveda)

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Background: Gouania longipetala Hemsl. is a scandent shrub used in traditional medicine for the management of different ailments such as gout, female infertility, heart diseases, diabetes, and malaria. Pathophysiology of the diseases is generally associated with oxidative stress. Antioxidant properties of G. longipetala have not yet been well elucidated. Aims: This study aimed to evaluate the antioxidant content and antioxidant activity of methanol extracts from the plant’s stems and leaves. Materials and methods: Methanol extracts from the plant stems and leaves were screened for phytochemical components with antioxidant properties (Vitamin C, total phenolics, and flavonoids). The antioxidant reducing power of the extracts as well as its effects on lipid peroxidation and catalase were also evaluated in vitro using biochemical assays. Results: Leaf extract was richer than stem extract in different antioxidants such as Vitamin C, phenolics, and flavonoids. Both leaf and stem extracts showed similar inhibitory effect on lipid peroxidation and stimulatory effect on catalase activity, while leaf extract had higher ferric antioxidant effect than stem extract. Conclusion: Both extracts from G. Longipetala possessed antioxidant content, though activities with leaf extract displayed higher antioxidant properties than stem extract.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antioxidant content and activity of stem and leaf extracts of Gouania longipetala Hemsl. (Rhamnaceae)

Soh,

Choumessi,

Tsague Manfo

et al. 2022

AYU (An International Quarterly Journal of Research in Ayurveda)

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“…Scopolamine-induced AD-like conditions in ovariectomized rats have long been used to demonstrate the neuroprotective effects of xenobiotic substances [ 34 , 35 ]. However, none of these studies lasted 84 days after ovariectomy, which is known to be the required time for the setup of postmenopause-like conditions in rats [ 19 – 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…30 rats were bilaterally ovariectomized using the dorsal approach, and 10 others were sham-operated. Animals were allowed for (84) days for the setup of menopausal-like conditions [ 19 – 21 ], followed by the random distribution of animals (now aged 5.5 months) into eight groups ( n = 5). Groups I (SHAM 1) and II (SHAM 2) were sham-operated rats and received distilled water.…”

Section: Methodsmentioning

confidence: 99%

Effects of Ficus umbellata (Moraceae) Aqueous Extract and 7-Methoxycoumarin on Scopolamine-Induced Spatial Memory Impairment in Ovariectomized Wistar Rats

Zingue

Foyet

Djiogue

et al. 2018

Behavioural Neurology

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The present work was undertaken to evaluate the ability of F. umbellata aqueous extract and its major component 7-methoxycoumarin (MC) to improve scopolamine-induced spatial memory impairment in ovariectomized Wistar rats. For this to be done, 10 sham-operated and 30 postmenopausal-like rats were randomly distributed in eight groups (n = 5) and treated with distilled water (2 mL/250 g), estradiol valerate (1 mg/kg BW), piracetam (1.5 mg/kg BW), F. umbellata aqueous extract (50 and 200 mg/kg BW), or MC (1 mg/kg BW) for 21 consecutive days. Before and after the memory impairment with scopolamine (2 mg/kg BW), animals underwent behavioral evaluations on Y- and radial mazes. As results, age and ovariectomy did not induce significant changes in the reference memory errors. While age decreased working memory errors, ovariectomy increased it. The MC as well as F. umbellata extract significantly increased (p < 0.01) the percentage of spontaneous alternation and decreased (p < 0.001) working and spatial reference memory errors and anxiety parameters (rearing and grooming) in ovariectomized rats. MC significantly reduced (p < 0.05) the MDA level, but resulted in an increase in GSH level in brain homogenates. These results suggest that MC is endowed with neuroprotective effects and could account for the neuroprotective effects of F. umbellata in rats.

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“…1b ). Eighty four (84) days after ovariectomy after the onset of postmenopausal-like conditions in rat [ 21 ], animals were randomly distributed into six groups of 5 animals each ( n = 5). Group I (SHAM) was sham operated rats and received distilled water.…”

Section: Methodsmentioning

confidence: 99%

Estrogen-like and tissue-selective effects of 7-methoxycoumarin from Ficus umbellata (Moraceae): an in vitro and in vivo study

Zingue

Michel

Nde

et al. 2017

BMC Complement Altern Med

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Background Ficus umbellata is a medicinal plant previously shown to endow estrogenic properties. Its major component was isolated and characterized as 7-methoxycoumarin (MC). Noteworthy, coumarins and the respective active metabolite 7-hydroxycoumarin analogs have shown aromatase inhibitory activity, which is of particular interest in the treatment of estrogen-dependent cancers. The present work aimed at evaluating the estrogenic/antiestrogenic effects of MC in vitro and in vivo.MethodsTo do so, in vitro assays using E-screen and reporter gene were done. In vivo, a 3-day uterotrophic assay followed by a postmenopausal-like rat model to characterize MC as well as F. umbellata aqueous extract in ovariectomized Wistar rats was performed. The investigations focused on histological (vaginal and uterine epithelial height) and morphological (uterine wet weight, vagina stratification and cornification) endpoints, bone mass, biochemical parameters and lipid profile.ResultsMC induced a significant (p < 0.05) MCF-7 cell proliferation at a concentration of 0.1 μM, but did not inhibit the effect induced by estradiol in both E-screen and reporter gene assays. In vivo, MC treatment did not show an uterotrophic effect in both rat models used. However, MC (1 mg/kg) induced a significant increase (p < 0.01) of vaginal epithelial height. No significant change was observed with MC in abdominal fat weight, serum lipid levels and bone weight.ConclusionThese results suggest that MC has a weak estrogenic activity in vitro and in vivo that accounts only in part to the estrogenicity of the whole plant extract. MC could be beneficial with regard to vagina dryness as it showed a tissue specific effect without exposing the uterus to a potential tumorigenic growth.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-017-1895-9) contains supplementary material, which is available to authorized users.

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In vivo estrogenic-like activities of Gouania longipetala Hemsl. (Rhamnaceae) bark extracts in a post-menopause-like model of ovariectomized Wistar rats

Cited by 14 publications

References 27 publications

Antioxidant content and activity of stem and leaf extracts of Gouania longipetala Hemsl. (Rhamnaceae)

Antioxidant content and activity of stem and leaf extracts of Gouania longipetala Hemsl. (Rhamnaceae)

Effects of Ficus umbellata (Moraceae) Aqueous Extract and 7-Methoxycoumarin on Scopolamine-Induced Spatial Memory Impairment in Ovariectomized Wistar Rats

Estrogen-like and tissue-selective effects of 7-methoxycoumarin from Ficus umbellata (Moraceae): an in vitro and in vivo study

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