In vivo evaluation of a metronidazole-containing gel for the adjuvant treatment of chronic periodontitis: preliminary results

Abstract

The present study developed an experimental metronidazole-based gel and evaluated its efficacy for the adjuvant treatment of chronic periodontitis. Sixteen patients were randomly allocated into two groups of eight subjects according to the following proposed treatments: (1) scaling and root planing (active control) or (2) scaling and root planing and direct periodontal intrapocket application of 15% metronidazole-based gel in two sites (≥5 mm in depth) (experimental group). Potential changes in the subgingival microbiota were assessed using a DNA Checkerboard method at three proposed times: baseline and following 7 or 30 days of drug administration. High-performance liquid chromatography (HPLC) monitored metronidazole concentrations in the crevicular fluid during treatment. The metronidazole experimental group presented lower bacterial counts than the control group at the three evaluated times (p<0.01 for baseline, p<0.001 for 7 or 30 days) when the target species were analyzed as a pool of bacteria. Samples revealed significantly lower counts 7 days after drug administration compared with baseline or after 30 days (p<0.05). HPLC analysis detected gel 1 h after application. The metronidazole-based gel significantly decreased the total bacterial count at the three evaluated times. Periodontopathogenic species were not different after gel administration.

The Monoglyceride Content Affects the Self-Assembly Behavior, Rheological Properties, Syringeability, and Mucoadhesion of In Situ–Gelling Liquid Crystalline Phase

“…[1][2][3][4] The viscosity of this type of system can be modulated by the amount of water it contains, 5 by temperature, and by pH changes 6 and can undergo a transition to gel on contact with the bodily fluid at a mucosal site. 1 The administration of the drug delivery system (DDS) into confined spaces, for example, within the vaginal 7,8 and nasal 1,9 cavities, periodontal pocket, 10,11 or conjunctival sac, 2 is a complex task and remains as a challenge for formulation scientists.…”

<i>In vitro</i> evaluation of electrospun PLGA/PLLA/PDLLA blend fibers loaded with naringin for guided bone regeneration

“…This may be due to strengthening by naringin, 6 Dent Mater J 2018; : -which acted as a crystal particle to enhance the polymer composite matrix 24) . Many resorbable drug delivery systems were developed during recent decades, such as drug loaded hydroxypropyl cellulose films 29) , which were first described by Noguchi et al, or drug carrying gels such as Elyzol ® (Dumex, Bad Vilbel, Germany) dental gel, based on melted glycerol mono-oleate 28,[30][31][32] . However, also for these systems, the periodontal milieu often poses the major problem that the required period of drug exposure (7 days) cannot be achieved 28,33) , let alone bone formation which would require several months.…”

Development and Utilization of Reversed Phase High Performance Liquid Chromatography Methods for a Series of Therapeutic Agents

“…The drug has also been shown to sensitize tumors to radiotherapy, opening its use in oncology [23]. For these conditions, the drug is often carried within a gel [24], with release from the gel being an important experimental variable. For this reason, HPLC is often employed as the drug salt is water soluble, has a strong chromophore, and is used in the described wide variety of applications.…”

“…For this reason, HPLC is often employed as the drug salt is water soluble, has a strong chromophore, and is used in the described wide variety of applications. Detection for this drug is performed over a wide range from 250 to 350 nm [22][23][24].…”