In Vivo evaluation of adipogenic induction in fibrous and honeycomb-structured atelocollagen scaffolds

Rodrı́guez, Augusto; Felice, Betiana; Sánchez, María Dolores Martín; Tsujigiwa, Hidetsugu; Felice, C.J.; Nagatsuka, Hitoshi

doi:10.1016/j.msec.2016.02.055

Cited by 2 publications

(2 citation statements)

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“…HSs with multichannel or aligned pores, providing sufficient porosity and pore interconnection and high enough mechanical resilience, attracted increasingly attention [20]. These have been fabricated by rapid prototyping, stainless steel needles/rings array templating and unidirectional freeze-drying [11][12][13][14][15][16][17][18][19]. Honeycomb structures with structural similarity to the spongy bone have been also prepared from ceramic or ceramic/polymer composites, with wood as a template and through different transformation routes [20 and references therein].…”

Section: Discussionmentioning

confidence: 99%

“…The last years, honeycomb-like architectures attracted increasingly attentions, providing high structural strength for relatively light and porous structures. Honeycomb structures made from atelocollagen were examined in vivo for their potential to induce the adipogenesys [19]. Honeycomb-like structures (HS) developed by biotemplating from poly(ε-caprolactone)/nanohydroxyapatite composites improved the attachment, proliferation and guided the growth of MG-63 osteoblast-like cells within the microchannels inside the structure [20].…”

Section: Introductionmentioning

confidence: 99%

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Laser-direct writing by two-photon polymerization of 3D honeycomb-like structures for bone regeneration

et al. 2018

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A major limitation of existing 3D implantable structures for bone tissue engineering is that most of the cells rapidly attach on the outer edges of the structure, restricting the cells penetration into the inner parts and causing the formation of a necrotic core. Furthermore, these structures generally possess a random spatial arrangement and do not preserve the isotropy on the whole volume. Here, we report on the fabrication and testing of an innovative 3D hierarchical, honeycomb-like structure (HS), with reproducible and isotropic arhitecture, that allows in 'volume' migration of osteoblasts. In particular, we demonstrate the possibility to control the 3D spatial cells growth inside these complex architectures by adjusting the free spaces inside the structures. The structures were made of vertical microtubes arranged in a mulitlayered configuration, fabricated via laser direct writing by two photons polymerization of the IP-L780 photopolymer. In vitro tests performed in MG-63 osteoblast-like cells demonstrated that the cells migration inside the 3D structures is conducted by the separation space between the microtubes layers. Specifically, for layers separation between 2 and 10 μm, the cells gradually penetrated between the microtubes. Furthermore, these structures induced the strongest cells osteogenic differentiation and mineralization, with ALP activity 1.5 times stronger, amount of calcified minerals 1.3 times higher and osteocalcin secretion increased by 2.3 times compared to the other structures. On the opposite, for layers separation less than 2 μm and above 10 μm, the cells were not able to make interconnections and exhibited poor mineralization ability.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Laser-direct writing by two-photon polymerization of 3D honeycomb-like structures for bone regeneration

et al. 2018

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Polymeric Materials, Advances and Applications in Tissue Engineering: A Review

et al. 2023

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Tissue Engineering (TE) is an interdisciplinary field that encompasses materials science in combination with biological and engineering sciences. In recent years, an increase in the demand for therapeutic strategies for improving quality of life has necessitated innovative approaches to designing intelligent biomaterials aimed at the regeneration of tissues and organs. Polymeric porous scaffolds play a critical role in TE strategies for providing a favorable environment for tissue restoration and establishing the interaction of the biomaterial with cells and inducing substances. This article reviewed the various polymeric scaffold materials and their production techniques, as well as the basic elements and principles of TE. Several interesting strategies in eight main TE application areas of epithelial, bone, uterine, vascular, nerve, cartilaginous, cardiac, and urinary tissue were included with the aim of learning about current approaches in TE. Different polymer-based medical devices approved for use in clinical trials and a wide variety of polymeric biomaterials are currently available as commercial products. However, there still are obstacles that limit the clinical translation of TE implants for use wide in humans, and much research work is still needed in the field of regenerative medicine.

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In Vivo evaluation of adipogenic induction in fibrous and honeycomb-structured atelocollagen scaffolds

Cited by 2 publications

References 38 publications

Laser-direct writing by two-photon polymerization of 3D honeycomb-like structures for bone regeneration

Laser-direct writing by two-photon polymerization of 3D honeycomb-like structures for bone regeneration

Polymeric Materials, Advances and Applications in Tissue Engineering: A Review

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