In Vivo Evaluation of Butylene Terephthalate-ethylene Oxide-DL, Lactide Polymer as Porous Scaffolds for Tissue Engineering

Abstract

The copolymers of poly(1,4-butylene terephthalate-co-ethylene oxide-co-DL-lactide), obtained by the transesterification reactions of poly(butylenes terephthalate) with poly(ethylene glycol DL-oligo(lactic acid), were fabricated into porous scaffolds by the established solvent-casting and particulate-leaching technique with NaCl as the porogen. The morphology of the porous scaffolds were investigated by the scanning electron microscopy (SEM), and the pores within the scaffold were proven to be interconnective ranging in size from 200 to 400 μm. The human bone marrow mesenchymal stem cells (MSC) seeded on the scaffolds were confirmed to survive and proliferate within the pores of the scaffold with the observation by immunofluorescence microscope and SEM. In vivo implantation of MSC-seeded scaffolds into athymic nude mice showed significant tissue formation in the subcutaneous sites of the immunodeficient mice at 3, 4, 6, and 9 weeks. The results indicate that the scaffolds were biocompatible with MSC and the host tissue in vitro and in vivo.