In vivo evaluation of cerium, gallium and vanadium-doped borate-based bioactive glass scaffolds using rat subcutaneous implantation model

Deliormanlı, Aylin M.; Vatansever, H. Seda; Yeşil, Hayrunnisa; Özdal-Kurt, F.

doi:10.1016/j.ceramint.2016.04.033

Cited by 30 publications

(26 citation statements)

References 28 publications

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“…A significant increase in cell number was not anticipated because the subcutaneous implant model is not an active fracture site where there is a plethora of cell signaling molecules that are released to attract cells to heal and remodel the site (Amini et al, ; Mistry & Mikos, ; Winkler, Sass, Duda, & Schmidt‐Bleek, ). Deliormanlı et al () studied the in vivo potential of a porous CNP/Bioglass composite scaffold and reported cell infiltration through the scaffold. Their scaffolds were made using a foaming technique that resulted in an average pore size of around 500 μm pores.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo biocompatibility assessment of free radical scavenging nanocomposite scaffolds for bone tissue regeneration

Dulany

Hepburn

Goins

et al. 2019

J Biomedical Materials Res

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Bone is the second most transplanted tissue in the world, resulting in increased demand for bone grafts leading to the fabrication of synthetic scaffold grafting alternatives. Fracture sites are under increased oxidative stress after injuries, affecting osteoblast function and hindering fracture healing and remodeling. To counter oxidative stress, free radical scavenging agents, such as cerium oxide nanoparticles, have gained traction in tissue engineering. Toward the goal of developing a functional synthetic system for bone tissue engineering, we characterized the biocompatibility of a porous, bioactive, free radical scavenging nanocomposite scaffold composed of poly (1,8 octanediol-co-citrate), beta-tricalcium phosphate, and cerium oxide nanoparticles. We studied cellular and tissue compatibility utilizing in vitro and in vivo models to assess nanocomposite interactions with both human osteoblast cells and rat subcutaneous tissue. We found the scaffolds were biocompatible in both models and supported cell attachment, proliferation, mineralization, and infiltration. Using hydrogen peroxide, we simulated oxidative stress to study the protective properties of the nanocomposite scaffolds via a reduction in cytotoxicity and recovered mineralization of osteoblast cells in vitro. We also found after implantation in vivo the scaffolds exhibited biocompatible properties essential for successful scaffolds for bone tissue engineering. Cells were able to infiltrate through the scaffolds, the surrounding tissues elicited a minimal immune response, and there were signs of scaffold degradation after 30 days of implantation.After the array of biological characterization, we had confirmed the development of a nanocomposite scaffold system capable of supporting bone-remodeling processes while providing a protective free radical scavenging effect. K E Y W O R D S biocompatibility, cerium, free radical scavenging, human osteoblasts, nanocomposite scaffolds, subcutaneous implant

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Section: Discussionmentioning

confidence: 99%

In vitro and in vivo biocompatibility assessment of free radical scavenging nanocomposite scaffolds for bone tissue regeneration

Dulany

Hepburn

Goins

et al. 2019

J Biomedical Materials Res

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“…When cerium and cerium oxide were doped into semiconducting nanomaterials, they functioned as inorganic disinfectants, with a higher antibacterial activity than pure semiconducting nanomaterials alone (Bomila et al, 2018). When cerium was doped into some scaffold materials such as HA, bioactive glass (BG), and metal organic frameworks (MOFs), these materials not only showed enhanced antibacterial properties but also achieved improved mechanical properties and biological properties (Gopi et al, 2014;Deliormanlı et al, 2016;Li et al, 2019a). The antimicrobial activities of cerium-and cerium oxide-doped materials are summarized in Table 3.…”

Section: Cerium-and Cerium Oxide-doped Materialsmentioning

confidence: 99%

Cerium and Its Oxidant-Based Nanomaterials for Antibacterial Applications: A State-of-the-Art Review

Zheng

et al. 2020

Front. Mater.

118

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Infectious diseases caused by bacteria remain a serious and global problem in human health. Herein, the discovery and application of antibiotics, which has attracted much attention in the past years, has helped to cure numerous infectious diseases and made huge contributions in the biomedical field. However, the widespread use of the broad-spectrum antibiotics has led to serious drug resistance for many human bacterial pathogens. In particular, bacterial drug resistance decreases therapeutic effects and leads to high mortality. Inspiringly, the introduction of nanomaterials in the biomedical field makes it possible to overcome the difficulty in bacterial drug resistance attributed to their unique antibacterial mechanism. Recently, a variety of metal-and metal oxide-based nanomaterials have been fully integrated in antibacterial applications and achieved excellent performances. Among them, cerium-and cerium oxide-based nanomaterials have received much attention worldwide. Remarkably, cerium oxide nanoparticles with lower toxicity act as effective antibacterial agents owing to their unique functional mechanism against pathogens through the reversible conversion of oxidation state between Ce(III) and Ce(IV). This article provides an overview of the state-of-the-art design and antibacterial applications of cerium-and cerium oxidebased materials. The first part discusses the underlying antibacterial mechanisms for cerium-and cerium oxide-based materials currently applied in biomedicine. The second part focuses on various antibacterial-related materials and their applications. In addition, the existing problems and future perspectives of the cerium-and cerium oxide-based materials make up the third part of this review. This paper could provide the possible mechanisms for antibacterial activities, various designs of cerium-and cerium oxide-based materials, and related antibacterial properties.

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“…Furthermore, several types of transition metal-doped soda lime silica glass have been prepared, and their bioactivity has been evaluated for biomedical applications 20 , 21 . Among the various vitreous compositions investigated, glass containing niobium oxide have received special attention, since the presence of niobium species has been associated with improvements in bioactive and mechanical properties 22 – 26 .…”

Section: Introductionmentioning

confidence: 99%

Comprehensive in vitro and in vivo studies of novel melt-derived Nb-substituted 45S5 bioglass reveal its enhanced bioactive properties for bone healing

Souza

Lopes

Encarnação

et al. 2018

Sci Rep

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The present work presents and discusses the results of a comprehensive study on the bioactive properties of Nb-substituted silicate glass derived from 45S5 bioglass. In vitro and in vivo experiments were performed. We undertook three different types of in vitro analyses: (i) investigation of the kinetics of chemical reactivity and the bioactivity of Nb-substituted glass in simulated body fluid (SBF) by 31P MASNMR spectroscopy, (ii) determination of ionic leaching profiles in buffered solution by inductively coupled plasma optical emission spectrometry (ICP-OES), and (iii) assessment of the compatibility and osteogenic differentiation of human embryonic stem cells (hESCs) treated with dissolution products of different compositions of Nb-substituted glass. The results revealed that Nb-substituted glass is not toxic to hESCs. Moreover, adding up to 1.3 mol% of Nb2O5 to 45S5 bioglass significantly enhanced its osteogenic capacity. For the in vivo experiments, trial glass rods were implanted into circular defects in rat tibia in order to evaluate their biocompatibility and bioactivity. Results showed all Nb-containing glass was biocompatible and that the addition of 1.3 mol% of Nb2O5, replacing phosphorous, increases the osteostimulation of bioglass. Therefore, these results support the assertion that Nb-substituted glass is suitable for biomedical applications.

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In vivo evaluation of cerium, gallium and vanadium-doped borate-based bioactive glass scaffolds using rat subcutaneous implantation model

Cited by 30 publications

References 28 publications

In vitro and in vivo biocompatibility assessment of free radical scavenging nanocomposite scaffolds for bone tissue regeneration

In vitro and in vivo biocompatibility assessment of free radical scavenging nanocomposite scaffolds for bone tissue regeneration

Cerium and Its Oxidant-Based Nanomaterials for Antibacterial Applications: A State-of-the-Art Review

Comprehensive in vitro and in vivo studies of novel melt-derived Nb-substituted 45S5 bioglass reveal its enhanced bioactive properties for bone healing

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