In Vivo Evaluation of Genotoxic Effect of Caralluma acutangula (Decne.) N.E.Br. extract on Mice Bone Marrow Cells

Al-Faifi, Zarraq

doi:10.26808/rs.st.i7v6.09

Cited by 3 publications

(5 citation statements)

References 12 publications

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“…The results confirmed the significant anticancer potential (IC 50 =38 µg/ml and 34 µg/ml respectively) of Ct capped metallic nanoparticles [51]. Other species of Caralluma such as Caralluma acutangula have also been reported for their excellent cytotoxic and anticancer activity against hepatocellular carcinoma (HEPG2) and breast cancer (MCF-7) cells [52]. Another study to evaluate the anticancer potential of methotrexate loaded chitosan nanoparticles was performed in HeLa cells.…”

Section: Figure 8) Cellular Uptake and Cytotoxicity Study Of Rhodamin...supporting

confidence: 60%

In vitro Anti-Cancer Effect of Polymeric Nanoparticles Encapsulating Caralluma tuberculata in Cancer Cells

Fayyaz,

Sarwar,

Nurjis

et al. 2023

Int J Adv Nano Comput Anal

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Rapidly evolving drug delivery systems employ therapeutic agents (liposomes, polymers, and nanospheres) to achieve optimum therapeutic and targeted effects with declined side effects to cure chronic diseases like cancer. Nano-formulation of a natural product i.e., Caralluma tuberculata (Ct) extract, has been used as an effective combinational therapy with enhanced biocompatibility owing to its strong anti-oxidant, anti-inflammatory, anti-bacterial, and anti-tumor potential. Ct extract was prepared using three solvents (EtOH, MeOH, and CHCl3) amongst which methanolic Ct extract exhibited the highest percentage yield (9.6%). Qualitative phytochemical screening, thin layer chromatography (TLC), and antioxidant assays (DPPH assay and H2O2 assay) were performed. The percentage free radical scavenging values were found to be 86.25% (IC50=140.1μg/ml) and 88% (IC50=14.22μg/ml) at 1000 μg/ml concentration for both assays respectively. Methanolic Ct extract was then encapsulated in chitosan-tripolyphosphate (CS-TPP) nanoparticles using ionic gelation method with an encapsulation efficiency of 87%. Characterization showed uniform size distribution of 140nm particle size using DLS and encapsulation of Ct extract inside CS-TPP nanoparticles was confirmed by UV spectrophotometry and FTIR. Ct loaded CS-TPP nanoparticles showed less than or equal to 5% hemolytic activity at concentrations of 15.62μg/ml, 31.25μg/ml, 62.5μg/ml, and 125μg/ml, suggesting its safer usage at lower concentration. Rhodamine conjugated Ct loaded CS-TPP nanoparticles showed significant uptake efficiency in breast cancer cells compared to control. Ct extract and the nanoformulation were treated against triple negative breast cancer cell lines (Cal-51) for the evaluation of cytotoxicity exhibiting 30-40% (IC50=122.3μg/ml) and up to 75% (IC50=14.39μg/ml) cytotoxicity respectively. The study paves way for the encapsulation of medicinal plants in polymeric nanoparticles to achieve safer and highly efficient drug delivery systems.

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Section: Figure 8) Cellular Uptake and Cytotoxicity Study Of Rhodamin...supporting

confidence: 60%

In vitro Anti-Cancer Effect of Polymeric Nanoparticles Encapsulating Caralluma tuberculata in Cancer Cells

Fayyaz,

Sarwar,

Nurjis

et al. 2023

Int J Adv Nano Comput Anal

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“…Furthermore, the obtained IC 50 values with FRAP test concerning hydroethanolic, flavonoids, saponins, and mucilages fractions were 5.196 mg/ml, 4.537 mg/ml, 3.05 mg/ml, and 6.02 mg/ml, respectively. ese results can 2 3 4 5 6 7 8 9 e methanolic extract of Caralluma acutangula exhibited effects with concentrations varying between 0 and 50 μg/ml on MCF-7 (breast cancer) and HEPG2 (hepatocellular carcinoma) [44]. e methanolic extracts of Caralluma acutangula possess an interesting antiproliferative activity on HeLa (cervix cancer), MCF7 (breast cancer), and HEPG2 (hepatocellular carcinoma) cells [45].…”

Section: Discussionmentioning

confidence: 98%

Phytochemical Study on Antioxidant and Antiproliferative Activities of Moroccan Caralluma europaea Extract and Its Bioactive Compound Classes

Amrati

Bourhia

Slighoua

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Caralluma europaea (C. europaea) is a medicinal plant used in Moroccan popular medicine. Objective of the Study. The present work was aimed at identifying the chemical composition and the antioxidant and antiproliferative activities of hydroethanolic and bioactive compound classes of C. europaea. Materials and Methods. The chemical composition was analyzed using HPLC. The antioxidant power was determined using both DPPH and FRAP assays. The antiproliferative activity was effectuated against cancerous cells using WST-1. Results. The chemical analysis showed the presence of bioactive constituents such as quercetin, myricetin, and hesperetin. The polyphenol and flavonoid contents were estimated at 51.42 mg GA/g and 20.06 mg EQ/g, respectively. The EC50 values of FRAP assay of hydroethanolic, flavonoid, saponin, and mucilage extracts were 5.196 mg/ml, 4.537 mg/ml, 3.05 mg/ml, and 6.02 mg/ml, respectively. The obtained IC50 values with the DPPH test were 1.628 mg/ml, 1.05 mg/ml, 1.94 mg/ml, and 9.674 mg/ml, respectively. Regarding MDA-MB-231, saponins were highly effective even with the lowest concentration (15.62 μg/ml). The flavonoids decreased the cell viability with IC50 values of 43.62 ± 0.06 μg/ml, whereas the hydroethanolic extract and mucilages were ineffective. Saponins were also effective against MCF-7 cells with IC50 values of 4.195 ± 0.025 μg/ml. Conclusion. The present results suggest that C. europaea may have considerable potential for the development of effective natural anticancer agents.

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“…The results are presented in Table 1. Crude methanolic extract has excellent free radical scavenging with IC 50 7.89 µg mLG 1 which is comparable to ascorbic acid (IC 50 , 5.63 µg mLG 1 ), a very important antioxidant compound. Table 1 showed that by increasing concentration of E. cactus Ehrenb methanolic extract, the scavenging activities also increasing (50<100<150<200<250< 500 µg mLG 1 ).…”

Section: Scavenging Effect (%) At Different Concentrations* ---------...mentioning

confidence: 98%

“…Approximately 7.5×104 cells mLG 1 was seeded in a six-well tissue culture plate and different concentrations of the extract was added after 24 h. For the determination of growth rate, smaller aliquots were collected in 0.5 mL tubes, trypan blue (0.4%) was added to the cell suspension and the number of cells (viable-unstained and non-viable-blue) was counted using a hemocytometer. The media were not changed during the induction period.…”

Section: Determination Of Anticancer Activitiesmentioning

confidence: 99%

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In vitro Anticancer, Antioxidant and Antimicrobial Activities of Crude Methanolic Extract of Euphorbia cactus Ehrenb Plant

Al-Faifi¹

2019

International J. of Pharmacology

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Background and Objective: Euphorbiaceae is a wide spread family of genus Euphorbia, from that family, belongs more than 2000 species. The plants of this genus have been used for a long time in traditional medicine. The present investigation is focused on the anticancer and antioxidant potential and antimicrobial activity of the methanolic extract of Euphorbia cactus Ehrenb plant (E. cactus Ehrenb). Materials and Methods: The study was facilitated by collecting the plant sample and subjected to crude methanol extraction.The anticancer activity was examined by MTT assay against three cancer cell lines MCF-7, PC-3 and HEPG2. The antimicrobial activity was determined using the agar diffusion method and MIC-determination. The DPPH radical method was used for the determination of antioxidant activity. Results: Interesting cytotoxic activity was observed for the extract against MCF-7 cell line compared to the known anticancer drugs. The antioxidant activity of the extract showed highly inhibitory activity compared with ascorbic acid. In addition, the methanolic extract showed the highest activity against Gram-positive bacteria, Bacillus subtilis and Streptococcus pneumonia and Gram-negative bacteria, Escherichia coli, compared with the standard drug. The extract didnʼt exert any activity against the pathogenic yeast species (Candida albicans) under these screening conditions. Conclusion: The current study demonstrated for the first time that E. cactus Ehrenb methanolic extract has anticancer effects against cancer cells from 3 types of cancerous cell lines. This may be a promising finding for future cancer treatments and in the prevention of cancer using natural products.

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In Vivo Evaluation of Genotoxic Effect of Caralluma acutangula (Decne.) N.E.Br. extract on Mice Bone Marrow Cells

Cited by 3 publications

References 12 publications

In vitro Anti-Cancer Effect of Polymeric Nanoparticles Encapsulating Caralluma tuberculata in Cancer Cells

In vitro Anti-Cancer Effect of Polymeric Nanoparticles Encapsulating Caralluma tuberculata in Cancer Cells

Phytochemical Study on Antioxidant and Antiproliferative Activities of Moroccan Caralluma europaea Extract and Its Bioactive Compound Classes

In vitro Anticancer, Antioxidant and Antimicrobial Activities of Crude Methanolic Extract of Euphorbia cactus Ehrenb Plant

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